Genomagnetic LAMP-based electrochemical test for determination
of high-risk HPV16 and HPV18 in clinical samples

J 2018

Genomagnetic LAMP-based electrochemical test for determination of high-risk HPV16 and HPV18 in clinical samples

BARTOŠÍK, Martin, Ludmila JIRÁKOVÁ, Milan ANTON, Bořivoj VOJTĚŠEK, Roman HRSTKA et. al.

Basic information

Original name

Genomagnetic LAMP-based electrochemical test for determination of high-risk HPV16 and HPV18 in clinical samples

Authors

BARTOŠÍK, Martin (703 Slovakia, guarantor), Ludmila JIRÁKOVÁ (203 Czech Republic), Milan ANTON (203 Czech Republic, belonging to the institution), Bořivoj VOJTĚŠEK (203 Czech Republic) and Roman HRSTKA (203 Czech Republic)

Edition

Analytica Chimica Acta, Amsterdam, Elsevier Science publishers, 2018, 0003-2670

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30214 Obstetrics and gynaecology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.256

RIV identification code

RIV/00216224:14110/18:00105867

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.aca.2018.08.020

UT WoS

000448290800005

Keywords in English

HPV; Cervical cancer; Genomagnetic assay; Electrode chip; LAMP amplification

Abstract

V originále

Major cause of cervical cancer is a persistent infection with high-risk types of human papillomaviruses (HPV). For that reason, HPV testing is now becoming an important addition to standard cytological screening of cervical malignancies in women (known as Pap test). New methods are sought which could offer rapid and inexpensive detection schemes, such as those based on electrochemical (EC) readout. Here, we developed an assay for parallel detection of two most oncogenic high-risk HPV types, HPV16 and HPV18, by combining loop-mediated amplification (LAMP) of template DNA, its separation using magnetic beads and detection with amperometry at carbon-based electrode chips. Our EC-LAMP test enabled us to successfully discriminate both HPV types not only in cancer cell lines, but also using clinical material obtained from HPV-positive patient samples. (C 2018 Elsevier B.V. All rights reserved.

Citovat

BARTOŠÍK, Martin, Ludmila JIRÁKOVÁ, Milan ANTON, Bořivoj VOJTĚŠEK and Roman HRSTKA. Genomagnetic LAMP-based electrochemical test for determination of high-risk HPV16 and HPV18 in clinical samples. Analytica Chimica Acta. Amsterdam: Elsevier Science publishers, 2018, vol. 1042, DEC 26 2018, p. 37-43. ISSN 0003-2670. Available from: https://dx.doi.org/10.1016/j.aca.2018.08.020.

@article{1490403,
   author = {Bartošík, Martin and Jiráková, Ludmila and Anton, Milan and Vojtěšek, Bořivoj and Hrstka, Roman},
   article_location = {Amsterdam},
   article_number = {DEC 26 2018},
   doi = {http://dx.doi.org/10.1016/j.aca.2018.08.020},
   keywords = {HPV; Cervical cancer; Genomagnetic assay; Electrode chip; LAMP amplification},
   language = {eng},
   issn = {0003-2670},
   journal = {Analytica Chimica Acta},
   title = {Genomagnetic LAMP-based electrochemical test for determination of high-risk HPV16 and HPV18 in clinical samples},
   url = {https://www.sciencedirect.com/science/article/pii/S0003267018309723},
   volume = {1042},
   year = {2018}
}

TY  - JOUR
ID  - 1490403
AU  - Bartošík, Martin - Jiráková, Ludmila - Anton, Milan - Vojtěšek, Bořivoj - Hrstka, Roman
PY  - 2018
TI  - Genomagnetic LAMP-based electrochemical test for determination of high-risk HPV16 and HPV18 in clinical samples
JF  - Analytica Chimica Acta
VL  - 1042
IS  - DEC 26 2018
SP  - 37-43
EP  - 37-43
PB  - Elsevier Science publishers
SN  - 00032670
KW  - HPV
KW  - Cervical cancer
KW  - Genomagnetic assay
KW  - Electrode chip
KW  - LAMP amplification
UR  - https://www.sciencedirect.com/science/article/pii/S0003267018309723
N2  - Major cause of cervical cancer is a persistent infection with high-risk types of human papillomaviruses (HPV). For that reason, HPV testing is now becoming an important addition to standard cytological screening of cervical malignancies in women (known as Pap test). New methods are sought which could offer rapid and inexpensive detection schemes, such as those based on electrochemical (EC) readout. Here, we developed an assay for parallel detection of two most oncogenic high-risk HPV types, HPV16 and HPV18, by combining loop-mediated amplification (LAMP) of template DNA, its separation using magnetic beads and detection with amperometry at carbon-based electrode chips. Our EC-LAMP test enabled us to successfully discriminate both HPV types not only in cancer cell lines, but also using clinical material obtained from HPV-positive patient samples. (C 2018 Elsevier B.V. All rights reserved.
ER  -

BARTOŠÍK, Martin, Ludmila JIRÁKOVÁ, Milan ANTON, Bořivoj VOJTĚŠEK and Roman HRSTKA. Genomagnetic LAMP-based electrochemical test for determination of high-risk HPV16 and HPV18 in clinical samples. \textit{Analytica Chimica Acta}. Amsterdam: Elsevier Science publishers, 2018, vol.~1042, DEC 26 2018, p.~37-43. ISSN~0003-2670. Available from: https://dx.doi.org/10.1016/j.aca.2018.08.020.

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