Nanodiamonds as

J 2018

Nanodiamonds as "artificial proteins": Regulation of a cell signalling system using low nanomolar solutions of inorganic nanocrystals

BÁLEK, Lukáš, Marcela BUCHTOVÁ, Michaela BOSÁKOVÁ, Miroslav VAŘECHA, Silvie TRANTÍRKOVÁ et. al.

Basic information

Original name

Nanodiamonds as "artificial proteins": Regulation of a cell signalling system using low nanomolar solutions of inorganic nanocrystals

Authors

BÁLEK, Lukáš (203 Czech Republic, belonging to the institution), Marcela BUCHTOVÁ (203 Czech Republic, belonging to the institution), Michaela BOSÁKOVÁ (203 Czech Republic, belonging to the institution), Miroslav VAŘECHA (203 Czech Republic, belonging to the institution), Silvie TRANTÍRKOVÁ (203 Czech Republic, belonging to the institution), Iva GUDERNOVÁ (203 Czech Republic, belonging to the institution), Iva VESELÁ (203 Czech Republic, belonging to the institution), Jan HAVLIK (203 Czech Republic), Jitka NEBURKOVA (203 Czech Republic), Stuart TURNER (56 Belgium), Mateusz Adam KRZYSCIK (616 Poland), Malgorzata ZAKRZEWSKA (56 Belgium), Lars KLIMASCHEWSKI (40 Austria), Peter CLAUS (276 Germany), Lukáš TRANTÍREK (203 Czech Republic, belonging to the institution), Petr CIGLER (203 Czech Republic) and Pavel KREJČÍ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Biomaterials, OXFORD, ELSEVIER SCI LTD, 2018, 0142-9612

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30404 Biomaterials

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 10.273

RIV identification code

RIV/00216224:14110/18:00101655

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.biomaterials.2018.05.030

UT WoS

000437038400010

Keywords in English

Nanodiamonds; Cell signalling; FGF; Fibroblast growth factor; Nanotherapeutics

Abstract

V originále

The blocking of specific protein-protein interactions using nanoparticles is an emerging alternative to small molecule-based therapeutic interventions. However, the nanoparticles designed as "artificial proteins" generally require modification of their surface with (bio)organic molecules and/or polymers to ensure their selectivity and specificity of action. Here, we show that nanosized diamond crystals (nanodiamonds, NDs) without any synthetically installed (bio)organic interface enable the specific and efficient targeting of the family of extracellular signalling molecules known as fibroblast growth factors (FGFs). We found that low nanomolar solutions of detonation NDs with positive-potential strongly associate with multiple FGF ligands present at sub-nanomolar concentrations and effectively neutralize the effects of FGF signalling in cells without interfering with other growth factor systems and serum proteins unrelated to FGFs. We identified an evolutionarily conserved FGF recognition motif, 17 amino acids long, that contributes to the selectivity of the ND-FGF interaction. In addition, we inserted this motif into a de novo constructed chimeric protein, which significantly improved its interaction with NDs. We demonstrated that the interaction of NDs, as purely inorganic nanoparticles, with proteins can mitigate pathological FGF signalling and promote the restoration of cartilage growth in a mouse limb explant model. Based on our observations, we foresee that NDs may potentially be applied as nano therapeutics to neutralize disease-related activities of FGFs in vivo. (C) 2018 The Authors. Published by Elsevier Ltd.

Links

GA17-09525S, research and development project

Name: Neobvyklé signální dráhy lidských receptorových tyrozinových kináz

Investor: Czech Science Foundation

GA17-12075S, research and development project

Name: Polymorfní G-quadruplexy v promotorových oblastech genů

Investor: Czech Science Foundation

LH15231, research and development project

Name: Nové mechanismy vzniku fatálních kostních ciliopatií u člověka

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

NV15-33232A, research and development project

Name: Identifikace nových možností léčby achondroplásie prostřednictvím analýzy interakce FGFR3 a adaptérového proteinu Frs2

NV15-34405A, research and development project

Name: Identifikace nových možností léčby chronické myeloidní leukémie pomocí systematické analýzy interaktomu proteinu BCR-ABL

Citovat

BÁLEK, Lukáš, Marcela BUCHTOVÁ, Michaela BOSÁKOVÁ, Miroslav VAŘECHA, Silvie TRANTÍRKOVÁ, Iva GUDERNOVÁ, Iva VESELÁ, Jan HAVLIK, Jitka NEBURKOVA, Stuart TURNER, Mateusz Adam KRZYSCIK, Malgorzata ZAKRZEWSKA, Lars KLIMASCHEWSKI, Peter CLAUS, Lukáš TRANTÍREK, Petr CIGLER and Pavel KREJČÍ. Nanodiamonds as "artificial proteins": Regulation of a cell signalling system using low nanomolar solutions of inorganic nanocrystals. Biomaterials. OXFORD: ELSEVIER SCI LTD, 2018, vol. 176, SEP 2018, p. 106-121. ISSN 0142-9612. Available from: https://dx.doi.org/10.1016/j.biomaterials.2018.05.030.

@article{1490507,
   author = {Bálek, Lukáš and Buchtová, Marcela and Bosáková, Michaela and Vařecha, Miroslav and Trantírková, Silvie and Gudernová, Iva and Veselá, Iva and Havlik, Jan and Neburkova, Jitka and Turner, Stuart and Krzyscik, Mateusz Adam and Zakrzewska, Malgorzata and Klimaschewski, Lars and Claus, Peter and Trantírek, Lukáš and Cigler, Petr and Krejčí, Pavel},
   article_location = {OXFORD},
   article_number = {SEP 2018},
   doi = {http://dx.doi.org/10.1016/j.biomaterials.2018.05.030},
   keywords = {Nanodiamonds; Cell signalling; FGF; Fibroblast growth factor; Nanotherapeutics},
   language = {eng},
   issn = {0142-9612},
   journal = {Biomaterials},
   title = {Nanodiamonds as "artificial proteins": Regulation of a cell signalling system using low nanomolar solutions of inorganic nanocrystals},
   url = {https://www.sciencedirect.com/science/article/pii/S0142961218303739?via%3Dihub},
   volume = {176},
   year = {2018}
}

TY  - JOUR
ID  - 1490507
AU  - Bálek, Lukáš - Buchtová, Marcela - Bosáková, Michaela - Vařecha, Miroslav - Trantírková, Silvie - Gudernová, Iva - Veselá, Iva - Havlik, Jan - Neburkova, Jitka - Turner, Stuart - Krzyscik, Mateusz Adam - Zakrzewska, Malgorzata - Klimaschewski, Lars - Claus, Peter - Trantírek, Lukáš - Cigler, Petr - Krejčí, Pavel
PY  - 2018
TI  - Nanodiamonds as "artificial proteins": Regulation of a cell signalling system using low nanomolar solutions of inorganic nanocrystals
JF  - Biomaterials
VL  - 176
IS  - SEP 2018
SP  - 106-121
EP  - 106-121
PB  - ELSEVIER SCI LTD
SN  - 01429612
KW  - Nanodiamonds
KW  - Cell signalling
KW  - FGF
KW  - Fibroblast growth factor
KW  - Nanotherapeutics
UR  - https://www.sciencedirect.com/science/article/pii/S0142961218303739?via%3Dihub
N2  - The blocking of specific protein-protein interactions using nanoparticles is an emerging alternative to small molecule-based therapeutic interventions. However, the nanoparticles designed as "artificial proteins" generally require modification of their surface with (bio)organic molecules and/or polymers to ensure their selectivity and specificity of action. Here, we show that nanosized diamond crystals (nanodiamonds, NDs) without any synthetically installed (bio)organic interface enable the specific and efficient targeting of the family of extracellular signalling molecules known as fibroblast growth factors (FGFs). We found that low nanomolar solutions of detonation NDs with positive-potential strongly associate with multiple FGF ligands present at sub-nanomolar concentrations and effectively neutralize the effects of FGF signalling in cells without interfering with other growth factor systems and serum proteins unrelated to FGFs. We identified an evolutionarily conserved FGF recognition motif, 17 amino acids long, that contributes to the selectivity of the ND-FGF interaction. In addition, we inserted this motif into a de novo constructed chimeric protein, which significantly improved its interaction with NDs. We demonstrated that the interaction of NDs, as purely inorganic nanoparticles, with proteins can mitigate pathological FGF signalling and promote the restoration of cartilage growth in a mouse limb explant model. Based on our observations, we foresee that NDs may potentially be applied as nano therapeutics to neutralize disease-related activities of FGFs in vivo. (C) 2018 The Authors. Published by Elsevier Ltd.
ER  -

BÁLEK, Lukáš, Marcela BUCHTOVÁ, Michaela BOSÁKOVÁ, Miroslav VAŘECHA, Silvie TRANTÍRKOVÁ, Iva GUDERNOVÁ, Iva VESELÁ, Jan HAVLIK, Jitka NEBURKOVA, Stuart TURNER, Mateusz Adam KRZYSCIK, Malgorzata ZAKRZEWSKA, Lars KLIMASCHEWSKI, Peter CLAUS, Lukáš TRANTÍREK, Petr CIGLER and Pavel KREJČÍ. Nanodiamonds as ''artificial proteins'': Regulation of a cell signalling system using low nanomolar solutions of inorganic nanocrystals. \textit{Biomaterials}. OXFORD: ELSEVIER SCI LTD, 2018, vol.~176, SEP 2018, p.~106-121. ISSN~0142-9612. Available from: https://dx.doi.org/10.1016/j.biomaterials.2018.05.030.

Detailed Information on Publication Record

Nanodiamonds as "artificial proteins": Regulation of a cell signalling system using low nanomolar solutions of inorganic nanocrystals

Basic information

Original name

Authors

Edition

Other information

Language

Type of outcome

Field of Study

Country of publisher

Confidentiality degree

References:

Impact factor

RIV identification code

Organization unit

DOI

UT WoS

Keywords in English

Tags

Tags

Abstract

V originále

Links