Detailed Information on Publication Record
2018
Nanodiamonds as "artificial proteins": Regulation of a cell signalling system using low nanomolar solutions of inorganic nanocrystals
BÁLEK, Lukáš, Marcela BUCHTOVÁ, Michaela BOSÁKOVÁ, Miroslav VAŘECHA, Silvie TRANTÍRKOVÁ et. al.Basic information
Original name
Nanodiamonds as "artificial proteins": Regulation of a cell signalling system using low nanomolar solutions of inorganic nanocrystals
Authors
BÁLEK, Lukáš (203 Czech Republic, belonging to the institution), Marcela BUCHTOVÁ (203 Czech Republic, belonging to the institution), Michaela BOSÁKOVÁ (203 Czech Republic, belonging to the institution), Miroslav VAŘECHA (203 Czech Republic, belonging to the institution), Silvie TRANTÍRKOVÁ (203 Czech Republic, belonging to the institution), Iva GUDERNOVÁ (203 Czech Republic, belonging to the institution), Iva VESELÁ (203 Czech Republic, belonging to the institution), Jan HAVLIK (203 Czech Republic), Jitka NEBURKOVA (203 Czech Republic), Stuart TURNER (56 Belgium), Mateusz Adam KRZYSCIK (616 Poland), Malgorzata ZAKRZEWSKA (56 Belgium), Lars KLIMASCHEWSKI (40 Austria), Peter CLAUS (276 Germany), Lukáš TRANTÍREK (203 Czech Republic, belonging to the institution), Petr CIGLER (203 Czech Republic) and Pavel KREJČÍ (203 Czech Republic, guarantor, belonging to the institution)
Edition
Biomaterials, OXFORD, ELSEVIER SCI LTD, 2018, 0142-9612
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30404 Biomaterials
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 10.273
RIV identification code
RIV/00216224:14110/18:00101655
Organization unit
Faculty of Medicine
UT WoS
000437038400010
Keywords in English
Nanodiamonds; Cell signalling; FGF; Fibroblast growth factor; Nanotherapeutics
Tags
International impact, Reviewed
Změněno: 20/4/2022 12:40, Mgr. Marie Šípková, DiS.
Abstract
V originále
The blocking of specific protein-protein interactions using nanoparticles is an emerging alternative to small molecule-based therapeutic interventions. However, the nanoparticles designed as "artificial proteins" generally require modification of their surface with (bio)organic molecules and/or polymers to ensure their selectivity and specificity of action. Here, we show that nanosized diamond crystals (nanodiamonds, NDs) without any synthetically installed (bio)organic interface enable the specific and efficient targeting of the family of extracellular signalling molecules known as fibroblast growth factors (FGFs). We found that low nanomolar solutions of detonation NDs with positive-potential strongly associate with multiple FGF ligands present at sub-nanomolar concentrations and effectively neutralize the effects of FGF signalling in cells without interfering with other growth factor systems and serum proteins unrelated to FGFs. We identified an evolutionarily conserved FGF recognition motif, 17 amino acids long, that contributes to the selectivity of the ND-FGF interaction. In addition, we inserted this motif into a de novo constructed chimeric protein, which significantly improved its interaction with NDs. We demonstrated that the interaction of NDs, as purely inorganic nanoparticles, with proteins can mitigate pathological FGF signalling and promote the restoration of cartilage growth in a mouse limb explant model. Based on our observations, we foresee that NDs may potentially be applied as nano therapeutics to neutralize disease-related activities of FGFs in vivo. (C) 2018 The Authors. Published by Elsevier Ltd.
Links
| GA17-09525S, research and development project |
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| GA17-12075S, research and development project |
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| LH15231, research and development project |
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| LQ1601, research and development project |
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| NV15-33232A, research and development project |
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| NV15-34405A, research and development project |
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