The aim of the study was to explore the relationship between parent-initiated motivational climate, motivation of young elite athletes in collective sports, and their influence on symptoms associated with overtraining syndrome. Our previous studies suggested a relationship between the parent-initiated motivational climate and the degree of internal and external motivation of the young elite athletes. We assumed that motivation could be a mediator of the relationship between the parent motivational climate and overtraining. The motivation was measured using the Sport Motivation Scale, 7-dimensional questionnaire based on the theory of self-determination. The extend of overtraining was indicated by Total Mood Disturbance score resulting from the Profile of Mood States. Further, the Parent-Initiated Motivational Climate Questionnaire (PIMCQ-2) was used. The research sample consisted of 230 boys aged 15-19 years (m=16.93; sd=1.81), classified as elite athletes competing at national or international level. Our study was focused on collective sports; soccer and ice hockey are among the most popular sports in the Czech Republic and have a wide base of young athletes. For this reason, our sample consisted of young footballers (58%) and hockey players (42%), selected by the convenient sampling method. The results linear regression analysis showed the significant impact of the motivation climate created by the father on self-determination of the athlete, but also on the mood disorder symptoms related to the overtraining syndrome. The influence of the mother-initiated motivational climate was not confirmed. It seems that the mastery father-initiated motivational climate increases the degree of self-determination and consequently reduces the rate of overtraining, while the performance motivational climate reduces self-determination, which is associated with an increased extend of overtraining. The results of the present study correspond with the conclusions of past studies that confirmed the significance of the influence of the father, especially in the case of the sporting sons.