Antimicrobial effect of novel hydrogel matrix based on natural
polysaccharide Sterculia urens

J 2018

Antimicrobial effect of novel hydrogel matrix based on natural polysaccharide Sterculia urens

LIPOVÝ, Břetislav, Jakub HOLOUBEK, Lukáš VACEK, Filip RŮŽIČKA, E. NEDOMOVA et. al.

Basic information

Original name

Antimicrobial effect of novel hydrogel matrix based on natural polysaccharide Sterculia urens

Authors

LIPOVÝ, Břetislav (203 Czech Republic, guarantor, belonging to the institution), Jakub HOLOUBEK (203 Czech Republic, belonging to the institution), Lukáš VACEK (203 Czech Republic, belonging to the institution), Filip RŮŽIČKA (203 Czech Republic, belonging to the institution), E. NEDOMOVA (203 Czech Republic), H. POŠTULKOVÁ (203 Czech Republic) and L. VOJTOVÁ (203 Czech Republic)

Edition

Čs. epidemiologie, mikrobiologie, imunologie, Praha, ČSL JEP, 2018, 1210-7913

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.397

RIV identification code

RIV/00216224:14110/18:00105964

Organization unit

Faculty of Medicine

UT WoS

000455791100003

Keywords in English

Gum Karaya; hydrogel; antimicrobial activity; wound healing; burn wounds

Abstract

V originále

Introduction: Materials for modern wound-management are a very broad and heterogeneous group. One of the most important representatives is natural materials, or more precisely polysaccharides isolated from various plants and animals. With the increasing resistance of pathogens to established antimicrobial agents, there is also an attempt to discover new mechanisms of the effects of these materials. Gum karaya (GK) is a very promising representative of the natural polysaccharides group and, since it is obtained from Sterculla urens as resin, it is also possible to assume its certain antimicrobial activity. Material and methodology: The antimicrobial potential of GK and chitosan (Ch) has been tested on several preselected strains to match the real epidemiological situation of the agents of infectious complications in the field of burned wounds. Tested strains included representatives of gram-positive and gram-negative bacteria as well as selected yeasts. Methicillin susceptible Staphylococcus aureus CCM 4223 (ATCC 29213), methicillin resistant Staphylococcus aureus CCM 4750 (ATCC 43300), Klebsiella pneumoniae CCM 4985 (ATCC 700603). Candida albicans CCM 8261 (ATCC 90028), Pseudomonas aeruginosa CCM 3955 (ATCC 27853) were obtained from the Czech Collection of Microorganisms. Pseudomonas aeruginosa FF 1, Pseudomonas aeruginosa FF 2 and Pseudomonas aeruginosa FF 3 (all multi-resistant clinical strains), Staphylococcus epidermidis A 013, Staphylococcus epidermidis A 117, and Candida parapsilosis BC 11 were obtained from the Collection of Microorganisms at the St. Anne's University Hospital, Brno. Antimicrobial tests were performed using the disk diffusion test methodology. Another set of antimicrobial tests was obtained by measuring the growth curves. Results: Bacteriostatic activity testing showed 1% GK concentration and both 1% and 0.5% chitosan concentration effective against all pathogens tested. The combination of GK s iCh, in concentrations of 1% and 0.5% had similar or better effect. Lower concentrations of the combined material are poorly effective against tested strains. Bactericidal activity testing has not produced positive results, except for Candida spp., where only a partial effect of GK(50)/Ch(50) was observed at 1% concentration. In the growth curve test, the efficiency of both GK alone and chitosan was found to be significantly higher in gram-positive bacteria compared to gram-negative ones. In the case of this experiment, only a one-tenth concentration was used compared to the disk diffusion test concentration. This results correspond with the data from the bacteriostatic activity testing. Conclusion: This is the first publication that attempts to comprehensively define the potential for GK antimicrobial activity and also the possible potentiation of this activity with the use of chitosan. Further experiments are needed to extend the antimicrobial efficiency to gram-negative bacteria.

Links

NV16-29916A, research and development project

Name: Využití bakteriofágů v léčbě nozokomiálních infekcí spojených s multirezistencí či tvorbou biofilmu

ROZV/25/LF/2017, interní kód MU

Name: LF - Příspěvek na IP 2017

Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects

Citovat

LIPOVÝ, Břetislav, Jakub HOLOUBEK, Lukáš VACEK, Filip RŮŽIČKA, E. NEDOMOVA, H. POŠTULKOVÁ and L. VOJTOVÁ. Antimicrobial effect of novel hydrogel matrix based on natural polysaccharide Sterculia urens. Čs. epidemiologie, mikrobiologie, imunologie. Praha: ČSL JEP, 2018, vol. 67, No 4, p. 166-174. ISSN 1210-7913.

@article{1491940,
   author = {Lipový, Břetislav and Holoubek, Jakub and Vacek, Lukáš and Růžička, Filip and Nedomova, E. and Poštulková, H. and Vojtová, L.},
   article_location = {Praha},
   article_number = {4},
   keywords = {Gum Karaya; hydrogel; antimicrobial activity; wound healing; burn wounds},
   language = {eng},
   issn = {1210-7913},
   journal = {Čs. epidemiologie, mikrobiologie, imunologie},
   title = {Antimicrobial effect of novel hydrogel matrix based on natural polysaccharide Sterculia urens},
   volume = {67},
   year = {2018}
}

TY  - JOUR
ID  - 1491940
AU  - Lipový, Břetislav - Holoubek, Jakub - Vacek, Lukáš - Růžička, Filip - Nedomova, E. - Poštulková, H. - Vojtová, L.
PY  - 2018
TI  - Antimicrobial effect of novel hydrogel matrix based on natural polysaccharide Sterculia urens
JF  - Čs. epidemiologie, mikrobiologie, imunologie
VL  - 67
IS  - 4
SP  - 166-174
EP  - 166-174
PB  - ČSL JEP
SN  - 12107913
KW  - Gum Karaya
KW  - hydrogel
KW  - antimicrobial activity
KW  - wound healing
KW  - burn wounds
N2  - Introduction: Materials for modern wound-management are a very broad and heterogeneous group. One of the most important representatives is natural materials, or more precisely polysaccharides isolated from various plants and animals. With the increasing resistance of pathogens to established antimicrobial agents, there is also an attempt to discover new mechanisms of the effects of these materials. Gum karaya (GK) is a very promising representative of the natural polysaccharides group and, since it is obtained from Sterculla urens as resin, it is also possible to assume its certain antimicrobial activity. Material and methodology: The antimicrobial potential of GK and chitosan (Ch) has been tested on several preselected strains to match the real epidemiological situation of the agents of infectious complications in the field of burned wounds. Tested strains included representatives of gram-positive and gram-negative bacteria as well as selected yeasts. Methicillin susceptible Staphylococcus aureus CCM 4223 (ATCC 29213), methicillin resistant Staphylococcus aureus CCM 4750 (ATCC 43300), Klebsiella pneumoniae CCM 4985 (ATCC 700603). Candida albicans CCM 8261 (ATCC 90028), Pseudomonas aeruginosa CCM 3955 (ATCC 27853) were obtained from the Czech Collection of Microorganisms. Pseudomonas aeruginosa FF 1, Pseudomonas aeruginosa FF 2 and Pseudomonas aeruginosa FF 3 (all multi-resistant clinical strains), Staphylococcus epidermidis A 013, Staphylococcus epidermidis A 117, and Candida parapsilosis BC 11 were obtained from the Collection of Microorganisms at the St. Anne's University Hospital, Brno. Antimicrobial tests were performed using the disk diffusion test methodology. Another set of antimicrobial tests was obtained by measuring the growth curves. Results: Bacteriostatic activity testing showed 1% GK concentration and both 1% and 0.5% chitosan concentration effective against all pathogens tested. The combination of GK s iCh, in concentrations of 1% and 0.5% had similar or better effect. Lower concentrations of the combined material are poorly effective against tested strains. Bactericidal activity testing has not produced positive results, except for Candida spp., where only a partial effect of GK(50)/Ch(50) was observed at 1% concentration. In the growth curve test, the efficiency of both GK alone and chitosan was found to be significantly higher in gram-positive bacteria compared to gram-negative ones. In the case of this experiment, only a one-tenth concentration was used compared to the disk diffusion test concentration. This results correspond with the data from the bacteriostatic activity testing. Conclusion: This is the first publication that attempts to comprehensively define the potential for GK antimicrobial activity and also the possible potentiation of this activity with the use of chitosan. Further experiments are needed to extend the antimicrobial efficiency to gram-negative bacteria.
ER  -

LIPOVÝ, Břetislav, Jakub HOLOUBEK, Lukáš VACEK, Filip RŮŽIČKA, E. NEDOMOVA, H. POŠTULKOVÁ and L. VOJTOVÁ. Antimicrobial effect of novel hydrogel matrix based on natural polysaccharide Sterculia urens. \textit{Čs. epidemiologie, mikrobiologie, imunologie}. Praha: ČSL JEP, 2018, vol.~67, No~4, p.~166-174. ISSN~1210-7913.

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