Monitoring Candida parapsilosis and Staphylococcus epidermidis
Biofilms by a Combination of Scanning Electron Microscopy and
Raman Spectroscopy

J 2018

Monitoring Candida parapsilosis and Staphylococcus epidermidis Biofilms by a Combination of Scanning Electron Microscopy and Raman Spectroscopy

HRUBANOVA, Kamila, Vladislav KRZYZANEK, Jana NEBESAROVA, Filip RŮŽIČKA, Zdenek PILAT et. al.

Basic information

Original name

Monitoring Candida parapsilosis and Staphylococcus epidermidis Biofilms by a Combination of Scanning Electron Microscopy and Raman Spectroscopy

Authors

HRUBANOVA, Kamila (203 Czech Republic), Vladislav KRZYZANEK (203 Czech Republic), Jana NEBESAROVA (203 Czech Republic), Filip RŮŽIČKA (203 Czech Republic, belonging to the institution), Zdenek PILAT (203 Czech Republic) and Ota SAMEK (203 Czech Republic, guarantor)

Edition

Sensors, Basel, MDPI AG, 2018, 1424-8220

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.031

RIV identification code

RIV/00216224:14110/18:00105966

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/s18124089

UT WoS

000454817100008

Keywords in English

Raman spectroscopy; biofilm; sample preparation; scanning electron microscopy; cryo-SEM

Abstract

V originále

The biofilm-forming microbial species Candida parapsilosis and Staphylococcus epidermidis have been recently linked to serious infections associated with implanted medical devices. We studied microbial biofilms by high resolution scanning electron microscopy (SEM), which allowed us to visualize the biofilm structure, including the distribution of cells inside the extracellular matrix and the areas of surface adhesion. We compared classical SEM (chemically fixed samples) with cryogenic SEM, which employs physical sample preparation based on plunging the sample into various liquid cryogens, as well as high-pressure freezing (HPF). For imaging the biofilm interior, we applied the freeze-fracture technique. In this study, we show that the different means of sample preparation have a fundamental influence on the observed biofilm structure. We complemented the SEM observations with Raman spectroscopic analysis, which allowed us to assess the time-dependent chemical composition changes of the biofilm in vivo. We identified the individual spectral peaks of the biomolecules present in the biofilm and we employed principal component analysis (PCA) to follow the temporal development of the chemical composition.

Links

LM2015062, research and development project

Name: Národní infrastruktura pro biologické a medicínské zobrazování

Investor: Ministry of Education, Youth and Sports of the CR

NV16-29916A, research and development project

Name: Využití bakteriofágů v léčbě nozokomiálních infekcí spojených s multirezistencí či tvorbou biofilmu

NV16-31593A, research and development project

Name: Polymikrobiální biofilm jako komplexní mikrobiom v rámci léčby nozokomiálních infekcí

Citovat

HRUBANOVA, Kamila, Vladislav KRZYZANEK, Jana NEBESAROVA, Filip RŮŽIČKA, Zdenek PILAT and Ota SAMEK. Monitoring Candida parapsilosis and Staphylococcus epidermidis Biofilms by a Combination of Scanning Electron Microscopy and Raman Spectroscopy. Sensors. Basel: MDPI AG, 2018, vol. 18, No 12, p. 1-19. ISSN 1424-8220. Available from: https://dx.doi.org/10.3390/s18124089.

@article{1491959,
   author = {Hrubanova, Kamila and Krzyzanek, Vladislav and Nebesarova, Jana and Růžička, Filip and Pilat, Zdenek and Samek, Ota},
   article_location = {Basel},
   article_number = {12},
   doi = {http://dx.doi.org/10.3390/s18124089},
   keywords = {Raman spectroscopy; biofilm; sample preparation; scanning electron microscopy; cryo-SEM},
   language = {eng},
   issn = {1424-8220},
   journal = {Sensors},
   title = {Monitoring Candida parapsilosis and Staphylococcus epidermidis Biofilms by a Combination of Scanning Electron Microscopy and Raman Spectroscopy},
   volume = {18},
   year = {2018}
}

TY  - JOUR
ID  - 1491959
AU  - Hrubanova, Kamila - Krzyzanek, Vladislav - Nebesarova, Jana - Růžička, Filip - Pilat, Zdenek - Samek, Ota
PY  - 2018
TI  - Monitoring Candida parapsilosis and Staphylococcus epidermidis Biofilms by a Combination of Scanning Electron Microscopy and Raman Spectroscopy
JF  - Sensors
VL  - 18
IS  - 12
SP  - 1-19
EP  - 1-19
PB  - MDPI AG
SN  - 14248220
KW  - Raman spectroscopy
KW  - biofilm
KW  - sample preparation
KW  - scanning electron microscopy
KW  - cryo-SEM
N2  - The biofilm-forming microbial species Candida parapsilosis and Staphylococcus epidermidis have been recently linked to serious infections associated with implanted medical devices. We studied microbial biofilms by high resolution scanning electron microscopy (SEM), which allowed us to visualize the biofilm structure, including the distribution of cells inside the extracellular matrix and the areas of surface adhesion. We compared classical SEM (chemically fixed samples) with cryogenic SEM, which employs physical sample preparation based on plunging the sample into various liquid cryogens, as well as high-pressure freezing (HPF). For imaging the biofilm interior, we applied the freeze-fracture technique. In this study, we show that the different means of sample preparation have a fundamental influence on the observed biofilm structure. We complemented the SEM observations with Raman spectroscopic analysis, which allowed us to assess the time-dependent chemical composition changes of the biofilm in vivo. We identified the individual spectral peaks of the biomolecules present in the biofilm and we employed principal component analysis (PCA) to follow the temporal development of the chemical composition.
ER  -

HRUBANOVA, Kamila, Vladislav KRZYZANEK, Jana NEBESAROVA, Filip RŮŽIČKA, Zdenek PILAT and Ota SAMEK. Monitoring Candida parapsilosis and Staphylococcus epidermidis Biofilms by a Combination of Scanning Electron Microscopy and Raman Spectroscopy. \textit{Sensors}. Basel: MDPI AG, 2018, vol.~18, No~12, p.~1-19. ISSN~1424-8220. Available from: https://dx.doi.org/10.3390/s18124089.

Detailed Information on Publication Record