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@article{1493186,
author = {Bardůnek Valigurová, Andrea and Pecková, Radka and Doležal, Karel and Sak, Bohumil and Květoňová, Dana and Kváč, Martin and Nurcahyo, Wisnu and Foitová, Ivona},
article_number = {010},
doi = {http://dx.doi.org/10.14411/fp.2018.010},
keywords = {Cryptosporidium gastric oocyst pathology treatment},
language = {eng},
issn = {0015-5683},
journal = {Folia Parasitologica},
title = {Limitations in the screening of potentially anti-cryptosporidial agents using laboratory rodents with gastric cryptosporidiosis},
url = {https://folia.paru.cas.cz/pdfs/fol/2018/01/10.pdf},
volume = {65},
year = {2018}
}
TY - JOUR
ID - 1493186
AU - Bardůnek Valigurová, Andrea - Pecková, Radka - Doležal, Karel - Sak, Bohumil - Květoňová, Dana - Kváč, Martin - Nurcahyo, Wisnu - Foitová, Ivona
PY - 2018
TI - Limitations in the screening of potentially anti-cryptosporidial agents using laboratory rodents with gastric cryptosporidiosis
JF - Folia Parasitologica
VL - 65
IS - 010
SP - 1-17
EP - 1-17
PB - AV ČR, Institute of Parasitology, Biology Centrum
SN - 00155683
KW - Cryptosporidium gastric oocyst pathology treatment
UR - https://folia.paru.cas.cz/pdfs/fol/2018/01/10.pdf
L2 - https://folia.paru.cas.cz/pdfs/fol/2018/01/10.pdf
N2 - Extracts of Indonesian plants, Piper betle and Diospyros sumatrana, were tested for potential anti-cryptosporidial activity using Mastomys coucha, experimentally inoculated with Cryptosporidium proliferans. None of the plant extracts tested showed significant activity against cryptosporidia; however, the results indicate that the following issues should be addressed in similar experimental studies. The monitoring of oocyst shedding during the entire experimental trial, supplemented with histological examination of affected gastric tissue at the time of treatment termination, revealed that similar studies are generally unreliable if evaluations of drug efficacy are based exclusively on oocyst shedding. Moreover, the reduction of oocyst shedding did not guarantee the eradication of cryptosporidia in treated individuals. For treatment trials performed on experimentally inoculated laboratory rodents, only animals in the advanced phase of cryptosporidiosis should be used for the correct interpretation of pathological alterations observed in affected tissue. All the solvents used (methanol, methanol-tetrahydrofuran and dimethylsulfoxid) were shown to be suitable for these studies, i.e. they did not exhibit negative effects on the subjects. The halofuginone lactate, routinely administered in intestinal cryptosporidiosis in calves, was shown to be ineffective against gastric cryptosporidiosis in mice caused by C. proliferans. In contrast, the control application of extract Arabidopsis thaliana, from which we had expected a neutral effect, turned out to have some positive impact on affected gastric tissue.
ER -
BARDŮNEK VALIGUROVÁ, Andrea, Radka PECKOVÁ, Karel DOLEŽAL, Bohumil SAK, Dana KVĚTOŇOVÁ, Martin KVÁČ, Wisnu NURCAHYO a Ivona FOITOVÁ. Limitations in the screening of potentially anti-cryptosporidial agents using laboratory rodents with gastric cryptosporidiosis. \textit{Folia Parasitologica}. AV ČR, Institute of Parasitology, Biology Centrum, 2018, roč.~65, č.~010, s.~1-17. ISSN~0015-5683. Dostupné z: https://dx.doi.org/10.14411/fp.2018.010.
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