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@proceedings{1494843, author = {Žákovská, Alena and Kučerová, Hana Lya and Marková, Jiřina and Bártová, Eva}, booktitle = {21th Conference on Laboratory Animals Scienceheld in Lednice (Czech Republic)}, keywords = {Detection antibodies Borrelia burgdorferi s.l. wild-type small mammals}, language = {eng}, title = {Detection of antibodies against Borrelia burgdorferi s.l. in wild-type small mammals and comparison of susceptibility of direct methods (PCR and cultivation)}, url = {https://www.biomed.cas.cz/physiolres/2018/5_18.htm}, year = {2018} }
TY - CONF ID - 1494843 AU - Žákovská, Alena - Kučerová, Hana Lya - Marková, Jiřina - Bártová, Eva PY - 2018 TI - Detection of antibodies against Borrelia burgdorferi s.l. in wild-type small mammals and comparison of susceptibility of direct methods (PCR and cultivation) KW - Detection antibodies Borrelia burgdorferi s.l. wild-type small mammals UR - https://www.biomed.cas.cz/physiolres/2018/5_18.htm L2 - https://www.biomed.cas.cz/physiolres/2018/5_18.htm N2 - The aim of this study was to determine the prevalence of antibodies against Borrelia burgdorferi s.l. in wild-type small mammals as well as to compare the sensitivity of direct detection methods (cultivation and PCR). Between 2010 and 2014, 691 wild small mammals were cought in three locations in the Czech Republic. Serum (n = 351) or cardiac rinse (n = 340) were examined with a modified indirect enzyme immunoassay (ELISA). Antibodies against B. burgdorferii s.l. were detected in 12% (484/691) animals. To compare the sensitivity of two direct methods, seventy random samples were used in 2012. B. burgdoferi s.l. was cultured in one sample (1.4%), which was also negative in PCR and ELISA. Using PCR, B. burgdoferi s.l. detected in seven samples (10%), two of them were dubious in ELISA. A total of 23 samples (11 positive and 12 dubious) had antibodies to B. burgdorferi s.l., but were also negative in PCR and cultivation. ER -
ŽÁKOVSKÁ, Alena, Hana Lya KUČEROVÁ, Jiřina MARKOVÁ a Eva BÁRTOVÁ. Detection of antibodies against Borrelia burgdorferi s.l. in wild-type small mammals and comparison of susceptibility of direct methods (PCR and cultivation). In \textit{21th Conference on Laboratory Animals Scienceheld in Lednice (Czech Republic)}. 2018. ISSN~0862-8408.
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