Proteomic characterization and antifungal activity of potato
tuber proteins isolated from starch production waste under
different temperature regimes

J 2018

Proteomic characterization and antifungal activity of potato tuber proteins isolated from starch production waste under different temperature regimes

BÁRTOVÁ, Veronika, Jan BÁRTA, Andrea VLAČIHOVÁ, Ondrej ŠEDO, Zbyněk ZDRÁHAL et. al.

Basic information

Original name

Proteomic characterization and antifungal activity of potato tuber proteins isolated from starch production waste under different temperature regimes

Authors

BÁRTOVÁ, Veronika (203 Czech Republic), Jan BÁRTA (203 Czech Republic), Andrea VLAČIHOVÁ (203 Czech Republic), Ondrej ŠEDO (203 Czech Republic, belonging to the institution), Zbyněk ZDRÁHAL (203 Czech Republic, guarantor, belonging to the institution), Hana KONEČNÁ (203 Czech Republic, belonging to the institution), Adéla STUPKOVÁ (203 Czech Republic) and Josef ŠVAJNER (203 Czech Republic)

Edition

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2018, 0175-7598

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.670

RIV identification code

RIV/00216224:14740/18:00106168

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1007/s00253-018-9373-y

UT WoS

000451523800017

Keywords in English

Potato; Solanum tuberosum L; Thermally stable proteins; Protease inhibitors; Antifungal activity

Abstract

V originále

Proteins were obtained from effluent of a starch manufacture by using different isolation temperatures (40, 60, 80, and 100 degrees C). The proteins, remaining in effluent after treatment of potato juice at 80 and 100 degrees C differed significantly in composition and in structural stability as well as in trypsin inhibitory and antifungal activities in comparison with the variants of 40 and 60 degrees C. The protein samples of 80 degrees C exhibited the highest antifungal activity and its average value of IC50 against five strains of two Fusarium species was determined in average at 0.18 mg ml(-1). The 80 degrees C protein samples consisted predominantly of low-molecular proteins (7-17 kDa) identified as potato tuber protease inhibitors I and II. Predominantly, protease inhibitors II were identified for the protein samples obtained by 100 degrees C and here we identified 7 spots in comparison with 12 identified for the 80 degrees C samples. Samples of 40 and 60 degrees C with low antifungal activities represent high variability of detected and identified proteins. We identified various representatives of aspartic, cysteine, and serine protease inhibitors in both types of samples. These samples also contained Kunitz-type protease inhibitors that were not found in the 80 and 100 degrees C samples which documented thermal unstableness of Kunitz-type protease inhibitors. Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures. At the same time, utilization of the stable protein fraction of waste deproteinized potato water promotes exploitation of potato starch production resources.

Links

LM2015043, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

BÁRTOVÁ, Veronika, Jan BÁRTA, Andrea VLAČIHOVÁ, Ondrej ŠEDO, Zbyněk ZDRÁHAL, Hana KONEČNÁ, Adéla STUPKOVÁ and Josef ŠVAJNER. Proteomic characterization and antifungal activity of potato tuber proteins isolated from starch production waste under different temperature regimes. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2018, vol. 102, No 24, p. 10551-10560. ISSN 0175-7598. Available from: https://dx.doi.org/10.1007/s00253-018-9373-y.

@article{1496258,
   author = {Bártová, Veronika and Bárta, Jan and Vlačihová, Andrea and Šedo, Ondrej and Zdráhal, Zbyněk and Konečná, Hana and Stupková, Adéla and Švajner, Josef},
   article_number = {24},
   doi = {http://dx.doi.org/10.1007/s00253-018-9373-y},
   keywords = {Potato; Solanum tuberosum L; Thermally stable proteins; Protease inhibitors; Antifungal activity},
   language = {eng},
   issn = {0175-7598},
   journal = {APPLIED MICROBIOLOGY AND BIOTECHNOLOGY},
   title = {Proteomic characterization and antifungal activity of potato tuber proteins isolated from starch production waste under different temperature regimes},
   volume = {102},
   year = {2018}
}

TY  - JOUR
ID  - 1496258
AU  - Bártová, Veronika - Bárta, Jan - Vlačihová, Andrea - Šedo, Ondrej - Zdráhal, Zbyněk - Konečná, Hana - Stupková, Adéla - Švajner, Josef
PY  - 2018
TI  - Proteomic characterization and antifungal activity of potato tuber proteins isolated from starch production waste under different temperature regimes
JF  - APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
VL  - 102
IS  - 24
SP  - 10551-10560
EP  - 10551-10560
SN  - 01757598
KW  - Potato
KW  - Solanum tuberosum L
KW  - Thermally stable proteins
KW  - Protease inhibitors
KW  - Antifungal activity
N2  - Proteins were obtained from effluent of a starch manufacture by using different isolation temperatures (40, 60, 80, and 100 degrees C). The proteins, remaining in effluent after treatment of potato juice at 80 and 100 degrees C differed significantly in composition and in structural stability as well as in trypsin inhibitory and antifungal activities in comparison with the variants of 40 and 60 degrees C. The protein samples of 80 degrees C exhibited the highest antifungal activity and its average value of IC50 against five strains of two Fusarium species was determined in average at 0.18 mg ml(-1). The 80 degrees C protein samples consisted predominantly of low-molecular proteins (7-17 kDa) identified as potato tuber protease inhibitors I and II. Predominantly, protease inhibitors II were identified for the protein samples obtained by 100 degrees C and here we identified 7 spots in comparison with 12 identified for the 80 degrees C samples. Samples of 40 and 60 degrees C with low antifungal activities represent high variability of detected and identified proteins. We identified various representatives of aspartic, cysteine, and serine protease inhibitors in both types of samples. These samples also contained Kunitz-type protease inhibitors that were not found in the 80 and 100 degrees C samples which documented thermal unstableness of Kunitz-type protease inhibitors. Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures. At the same time, utilization of the stable protein fraction of waste deproteinized potato water promotes exploitation of potato starch production resources.
ER  -

BÁRTOVÁ, Veronika, Jan BÁRTA, Andrea VLAČIHOVÁ, Ondrej ŠEDO, Zbyněk ZDRÁHAL, Hana KONEČNÁ, Adéla STUPKOVÁ and Josef ŠVAJNER. Proteomic characterization and antifungal activity of potato tuber proteins isolated from starch production waste under different temperature regimes. \textit{APPLIED MICROBIOLOGY AND BIOTECHNOLOGY}. 2018, vol.~102, No~24, p.~10551-10560. ISSN~0175-7598. Available from: https://dx.doi.org/10.1007/s00253-018-9373-y.

Detailed Information on Publication Record