| CALUORI, Guido, Jan PŘIBYL, Martin PEŠL, Šárka JELÍNKOVÁ, Vladimír ROTREKL, Petr SKLÁDAL a Roberto RAITERI. Non-invasive electromechanical cell-based biosensors for improved investigation of 3D cardiac models. Biosensors & bioelectronics : the international journal for the professional involved with research, technology and applications of biosensors and related devices. Elsevier Science, 2019, roč. 124, JAN 15 2019, s. 129-135. ISSN 0956-5663. Dostupné z: https://dx.doi.org/10.1016/j.bios.2018.10.021. |
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@article{1498076,
author = {Caluori, Guido and Přibyl, Jan and Pešl, Martin and Jelínková, Šárka and Rotrekl, Vladimír and Skládal, Petr and Raiteri, Roberto},
article_number = {JAN 15 2019},
doi = {http://dx.doi.org/10.1016/j.bios.2018.10.021},
keywords = {Excitation-contraction coupling; Human pluripotent stem cells; Cardiomyocytes; Atomic force microscopy; Microelectrode array; Drug testing},
language = {eng},
issn = {0956-5663},
journal = {Biosensors & bioelectronics : the international journal for the professional involved with research, technology and applications of biosensors and related devices},
title = {Non-invasive electromechanical cell-based biosensors for improved investigation of 3D cardiac models},
url = {https://www.sciencedirect.com/science/article/pii/S0956566318308315?via%3Dihub},
volume = {124},
year = {2019}
}
TY - JOUR
ID - 1498076
AU - Caluori, Guido - Přibyl, Jan - Pešl, Martin - Jelínková, Šárka - Rotrekl, Vladimír - Skládal, Petr - Raiteri, Roberto
PY - 2019
TI - Non-invasive electromechanical cell-based biosensors for improved investigation of 3D cardiac models
JF - Biosensors & bioelectronics : the international journal for the professional involved with research, technology and applications of biosensors and related devices
VL - 124
IS - JAN 15 2019
SP - 129-135
EP - 129-135
PB - Elsevier Science
SN - 09565663
KW - Excitation-contraction coupling
KW - Human pluripotent stem cells
KW - Cardiomyocytes
KW - Atomic force microscopy
KW - Microelectrode array
KW - Drug testing
UR - https://www.sciencedirect.com/science/article/pii/S0956566318308315?via%3Dihub
L2 - https://www.sciencedirect.com/science/article/pii/S0956566318308315?via%3Dihub
N2 - Cardiomyocytes (CM) placed on microelectrode array (MEA) were simultaneously probed with cantilever from atomic force microscope (AFM) system. This electric,nanomechanical combination in real time recorded beating force of the CMs cluster and the triggering electric events. Such "organ-on-a-chip" represents a tool for drug development and disease modeling. The human pluripotent stem cells included the WT embryonic line CCTL14 and the induced dystrophin deficient line reprogrammed from fibroblasts of a patient affected by Duchenne Muscular Dystrophy (DMD, complete loss of dystrophin expression). Both were differentiated to CMs and employed with the AFM/MEA platform for diseased CMs’ drug response testing and DMD characterization. The dependence of cardiac parameters on extracellular Ca2+ was studied. The differential evaluation explained the observed effects despite variability of biological samples. The beta-adrenergic stimulation (isoproterenol) and antagonist trials (verapamil) addressed ionotropic and chronotropic cell line-dependent features. For the first time, a distinctive beating-force relation for DMD CMs was measured on the 3D cardiac in vitro model.
ER -
CALUORI, Guido, Jan PŘIBYL, Martin PEŠL, Šárka JELÍNKOVÁ, Vladimír ROTREKL, Petr SKLÁDAL a Roberto RAITERI. Non-invasive electromechanical cell-based biosensors for improved investigation of 3D cardiac models. \textit{Biosensors \&{} bioelectronics : the international journal for the professional involved with research, technology and applications of biosensors and related devices}. Elsevier Science, 2019, roč.~124, JAN 15 2019, s.~129-135. ISSN~0956-5663. Dostupné z: https://dx.doi.org/10.1016/j.bios.2018.10.021.
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