CALUORI, Guido, Jan PŘIBYL, Martin PEŠL, Šárka JELÍNKOVÁ, Vladimír ROTREKL, Petr SKLÁDAL and Roberto RAITERI. Non-invasive electromechanical cell-based biosensors for improved investigation of 3D cardiac models. Biosensors & bioelectronics : the international journal for the professional involved with research, technology and applications of biosensors and related devices. Elsevier Science, 2019, vol. 124, JAN 15 2019, p. 129-135. ISSN 0956-5663. Available from: https://dx.doi.org/10.1016/j.bios.2018.10.021. |
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@article{1498076, author = {Caluori, Guido and Přibyl, Jan and Pešl, Martin and Jelínková, Šárka and Rotrekl, Vladimír and Skládal, Petr and Raiteri, Roberto}, article_number = {JAN 15 2019}, doi = {http://dx.doi.org/10.1016/j.bios.2018.10.021}, keywords = {Excitation-contraction coupling; Human pluripotent stem cells; Cardiomyocytes; Atomic force microscopy; Microelectrode array; Drug testing}, language = {eng}, issn = {0956-5663}, journal = {Biosensors & bioelectronics : the international journal for the professional involved with research, technology and applications of biosensors and related devices}, title = {Non-invasive electromechanical cell-based biosensors for improved investigation of 3D cardiac models}, url = {https://www.sciencedirect.com/science/article/pii/S0956566318308315?via%3Dihub}, volume = {124}, year = {2019} }
TY - JOUR ID - 1498076 AU - Caluori, Guido - Přibyl, Jan - Pešl, Martin - Jelínková, Šárka - Rotrekl, Vladimír - Skládal, Petr - Raiteri, Roberto PY - 2019 TI - Non-invasive electromechanical cell-based biosensors for improved investigation of 3D cardiac models JF - Biosensors & bioelectronics : the international journal for the professional involved with research, technology and applications of biosensors and related devices VL - 124 IS - JAN 15 2019 SP - 129-135 EP - 129-135 PB - Elsevier Science SN - 09565663 KW - Excitation-contraction coupling KW - Human pluripotent stem cells KW - Cardiomyocytes KW - Atomic force microscopy KW - Microelectrode array KW - Drug testing UR - https://www.sciencedirect.com/science/article/pii/S0956566318308315?via%3Dihub L2 - https://www.sciencedirect.com/science/article/pii/S0956566318308315?via%3Dihub N2 - Cardiomyocytes (CM) placed on microelectrode array (MEA) were simultaneously probed with cantilever from atomic force microscope (AFM) system. This electric,nanomechanical combination in real time recorded beating force of the CMs cluster and the triggering electric events. Such "organ-on-a-chip" represents a tool for drug development and disease modeling. The human pluripotent stem cells included the WT embryonic line CCTL14 and the induced dystrophin deficient line reprogrammed from fibroblasts of a patient affected by Duchenne Muscular Dystrophy (DMD, complete loss of dystrophin expression). Both were differentiated to CMs and employed with the AFM/MEA platform for diseased CMs’ drug response testing and DMD characterization. The dependence of cardiac parameters on extracellular Ca2+ was studied. The differential evaluation explained the observed effects despite variability of biological samples. The beta-adrenergic stimulation (isoproterenol) and antagonist trials (verapamil) addressed ionotropic and chronotropic cell line-dependent features. For the first time, a distinctive beating-force relation for DMD CMs was measured on the 3D cardiac in vitro model. ER -
CALUORI, Guido, Jan PŘIBYL, Martin PEŠL, Šárka JELÍNKOVÁ, Vladimír ROTREKL, Petr SKLÁDAL and Roberto RAITERI. Non-invasive electromechanical cell-based biosensors for improved investigation of 3D cardiac models. \textit{Biosensors \&{} bioelectronics : the international journal for the professional involved with research, technology and applications of biosensors and related devices}. Elsevier Science, 2019, vol.~124, JAN 15 2019, p.~129-135. ISSN~0956-5663. Available from: https://dx.doi.org/10.1016/j.bios.2018.10.021.
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