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@inbook{1498119,
author = {Přibyl, Jan and Pešl, Martin and Caluori, Guido and Aćimović, Ivana and Jelínková, Šárka and Dvořák, Petr and Skládal, Petr and Rotrekl, Vladimír},
address = {New York, NY},
booktitle = {Atomic Force Microscopy},
doi = {http://dx.doi.org/10.1007/978-1-4939-8894-5_20},
editor = {Nuno C. Santos; Filomena A. Carvalho},
keywords = {Atomic force microscopy; Biomechanical characterization; Human stem cell; Cardiomyocyte contraction; Drug testing},
howpublished = {tištěná verze "print"},
language = {eng},
location = {New York, NY},
isbn = {978-1-4939-8893-8},
pages = {343-353},
publisher = {Springer},
title = {Biomechanical Characterization of Human Pluripotent Stem Cell-Derived Cardiomyocytes by Use of Atomic Force Microscopy},
url = {https://link.springer.com/protocol/10.1007%2F978-1-4939-8894-5_20},
year = {2019}
}
TY - CHAP
ID - 1498119
AU - Přibyl, Jan - Pešl, Martin - Caluori, Guido - Aćimović, Ivana - Jelínková, Šárka - Dvořák, Petr - Skládal, Petr - Rotrekl, Vladimír
PY - 2019
TI - Biomechanical Characterization of Human Pluripotent Stem Cell-Derived Cardiomyocytes by Use of Atomic Force Microscopy
VL - Methods in Molecular Biology, volume 1886
PB - Springer
CY - New York, NY
SN - 9781493988938
KW - Atomic force microscopy
KW - Biomechanical characterization
KW - Human stem cell
KW - Cardiomyocyte contraction
KW - Drug testing
UR - https://link.springer.com/protocol/10.1007%2F978-1-4939-8894-5_20
L2 - https://link.springer.com/protocol/10.1007%2F978-1-4939-8894-5_20
N2 - Atomic force microscopy (AFM) is not only a high-resolution imaging technique but also a sensitive tool able to study biomechanical properties of bio-samples (biomolecules, cells) in native conditions—i.e., in buffered solutions (culturing media) and stable temperature (mostly 37 °C). Micromechanical transducers (cantilevers) are often used to map surface stiffness distribution, adhesion forces, and viscoelastic parameters of living cells; however, they can also be used to monitor time course of cardiomyocytes contraction dynamics (e.g. beating rate, relaxation time), together with other biomechanical properties. Here we describe the construction of an AFM-based biosensor setup designed to study the biomechanical properties of cardiomyocyte clusters, through the use of standard uncoated silicon nitride cantilevers. Force-time curves (mechanocardiograms, MCG) are recorded continuously in real time and in the presence of cardiomyocyte-contraction affecting drugs (e.g., isoproterenol, metoprolol) in the medium, under physiological conditions. The average value of contraction force and the beat rate, as basic biomechanical parameters, represent pharmacological indicators of different phenotype features. Robustness, low computational requirements, and optimal spatial sensitivity (detection limit 200 pN, respectively 20 nm displacement) are the main advantages of the presented method.
ER -
PŘIBYL, Jan, Martin PEŠL, Guido CALUORI, Ivana A$\backslash$'CIMOVI$\backslash$'C, Šárka JELÍNKOVÁ, Petr DVOŘÁK, Petr SKLÁDAL and Vladimír ROTREKL. Biomechanical Characterization of Human Pluripotent Stem Cell-Derived Cardiomyocytes by Use of Atomic Force Microscopy. In Nuno C. Santos; Filomena A. Carvalho. \textit{Atomic Force Microscopy}. New York, NY: Springer, 2019, p.~343-353. Methods in Molecular Biology, volume 1886. ISBN~978-1-4939-8893-8. Available from: https://dx.doi.org/10.1007/978-1-4939-8894-5\_{}20.
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