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@inbook{1498119, author = {Přibyl, Jan and Pešl, Martin and Caluori, Guido and Aćimović, Ivana and Jelínková, Šárka and Dvořák, Petr and Skládal, Petr and Rotrekl, Vladimír}, address = {New York, NY}, booktitle = {Atomic Force Microscopy}, doi = {http://dx.doi.org/10.1007/978-1-4939-8894-5_20}, editor = {Nuno C. Santos; Filomena A. Carvalho}, keywords = {Atomic force microscopy; Biomechanical characterization; Human stem cell; Cardiomyocyte contraction; Drug testing}, howpublished = {tištěná verze "print"}, language = {eng}, location = {New York, NY}, isbn = {978-1-4939-8893-8}, pages = {343-353}, publisher = {Springer}, title = {Biomechanical Characterization of Human Pluripotent Stem Cell-Derived Cardiomyocytes by Use of Atomic Force Microscopy}, url = {https://link.springer.com/protocol/10.1007%2F978-1-4939-8894-5_20}, year = {2019} }
TY - CHAP ID - 1498119 AU - Přibyl, Jan - Pešl, Martin - Caluori, Guido - Aćimović, Ivana - Jelínková, Šárka - Dvořák, Petr - Skládal, Petr - Rotrekl, Vladimír PY - 2019 TI - Biomechanical Characterization of Human Pluripotent Stem Cell-Derived Cardiomyocytes by Use of Atomic Force Microscopy VL - Methods in Molecular Biology, volume 1886 PB - Springer CY - New York, NY SN - 9781493988938 KW - Atomic force microscopy KW - Biomechanical characterization KW - Human stem cell KW - Cardiomyocyte contraction KW - Drug testing UR - https://link.springer.com/protocol/10.1007%2F978-1-4939-8894-5_20 L2 - https://link.springer.com/protocol/10.1007%2F978-1-4939-8894-5_20 N2 - Atomic force microscopy (AFM) is not only a high-resolution imaging technique but also a sensitive tool able to study biomechanical properties of bio-samples (biomolecules, cells) in native conditions—i.e., in buffered solutions (culturing media) and stable temperature (mostly 37 °C). Micromechanical transducers (cantilevers) are often used to map surface stiffness distribution, adhesion forces, and viscoelastic parameters of living cells; however, they can also be used to monitor time course of cardiomyocytes contraction dynamics (e.g. beating rate, relaxation time), together with other biomechanical properties. Here we describe the construction of an AFM-based biosensor setup designed to study the biomechanical properties of cardiomyocyte clusters, through the use of standard uncoated silicon nitride cantilevers. Force-time curves (mechanocardiograms, MCG) are recorded continuously in real time and in the presence of cardiomyocyte-contraction affecting drugs (e.g., isoproterenol, metoprolol) in the medium, under physiological conditions. The average value of contraction force and the beat rate, as basic biomechanical parameters, represent pharmacological indicators of different phenotype features. Robustness, low computational requirements, and optimal spatial sensitivity (detection limit 200 pN, respectively 20 nm displacement) are the main advantages of the presented method. ER -
PŘIBYL, Jan, Martin PEŠL, Guido CALUORI, Ivana A$\backslash$'CIMOVI$\backslash$'C, Šárka JELÍNKOVÁ, Petr DVOŘÁK, Petr SKLÁDAL and Vladimír ROTREKL. Biomechanical Characterization of Human Pluripotent Stem Cell-Derived Cardiomyocytes by Use of Atomic Force Microscopy. In Nuno C. Santos; Filomena A. Carvalho. \textit{Atomic Force Microscopy}. New York, NY: Springer, 2019, p.~343-353. Methods in Molecular Biology, volume 1886. ISBN~978-1-4939-8893-8. Available from: https://dx.doi.org/10.1007/978-1-4939-8894-5\_{}20.
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