| RESLOVÁ, Nikol, Veronika HUVAROVÁ, Jakub HRDÝ, Martin KAŠNÝ a Petr KRÁLÍK. A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay. Scientific reports. London: Nature Publishing Group, 2019, roč. 9, č. 2719, s. 1-13. ISSN 2045-2322. doi:10.1038/s41598-019-40035-5. |
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@article{1501505,
author = {Reslová, Nikol and Huvarová, Veronika and Hrdý, Jakub and Kašný, Martin and Králík, Petr},
article_location = {London},
article_number = {2719},
doi = {http://dx.doi.org/10.1038/s41598-019-40035-5},
keywords = {xMAP; multiplex detection; foodborne pathogens; optimization; MAGPIX},
language = {eng},
issn = {2045-2322},
journal = {Scientific reports},
title = {A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay},
url = {https://rdcu.be/boqEh},
volume = {9},
year = {2019}
}
TY - JOUR
ID - 1501505
AU - Reslová, Nikol - Huvarová, Veronika - Hrdý, Jakub - Kašný, Martin - Králík, Petr
PY - 2019
TI - A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay
JF - Scientific reports
VL - 9
IS - 2719
SP - 1-13
EP - 1-13
PB - Nature Publishing Group
SN - 20452322
KW - xMAP
KW - multiplex detection
KW - foodborne pathogens
KW - optimization
KW - MAGPIX
UR - https://rdcu.be/boqEh
L2 - https://rdcu.be/boqEh
N2 - Multiplex oligonucleotide ligation-PCR (MOL-PCR) is a rapid method for simultaneous detection of multiple molecular markers within a single reaction. MOL-PCR is increasingly employed in microbial detection assays, where its ability to facilitate identification and further characterization via simple analysis is of great benefit and significantly simplifies routine diagnostics. When adapted to microsphere suspension arrays on a MAGPIX reader, MOL-PCR has the potential to outperform standard nucleic acid-based diagnostic assays. This study represents the guideline towards in-house MOL-PCR assay optimization using the example of foodborne pathogens (bacteria and parasites) with an emphasis on the appropriate choice of crucial parameters. The optimized protocol focused on specific sequence detection utilizes the fluorescent reporter BODIPY-TMRX and self-coupled magnetic microspheres and allows for a smooth and brisk workflow which should serve as a guide for the development of MOL-PCR assays intended for pathogen detection.
ER -
RESLOVÁ, Nikol, Veronika HUVAROVÁ, Jakub HRDÝ, Martin KAŠNÝ a Petr KRÁLÍK. A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay. \textit{Scientific reports}. London: Nature Publishing Group, 2019, roč.~9, č.~2719, s.~1-13. ISSN~2045-2322. doi:10.1038/s41598-019-40035-5.
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