J 2018

Advances in the cellular structural biology of nucleic acids

GIASSA, Ilektra-Chara, Jan RYNEŠ, T. FESSL, Silvie TRANTÍRKOVÁ, Lukáš TRANTÍREK et. al.

Basic information

Original name

Advances in the cellular structural biology of nucleic acids

Authors

GIASSA, Ilektra-Chara (300 Greece, belonging to the institution), Jan RYNEŠ (203 Czech Republic, belonging to the institution), T. FESSL (203 Czech Republic), Silvie TRANTÍRKOVÁ (203 Czech Republic, belonging to the institution) and Lukáš TRANTÍREK (203 Czech Republic, guarantor, belonging to the institution)

Edition

FEBS LETTERS, HOBOKEN, WILEY, 2018, 1873-3468

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 2.675

RIV identification code

RIV/00216224:14740/18:00101748

Organization unit

Central European Institute of Technology

DOI

UT WoS

000436109600009

Keywords in English

DNA; in-cell chemical probing; in-cell EPR; in-cell NMR; in-cell single-molecule FRET; RNA

Tags

Tags

International impact, Reviewed
Změněno: 15/3/2019 13:58, Mgr. Pavla Foltynová, Ph.D.

Abstract

V originále

Conventional biophysical and chemical biology approaches for delineating relationships between the structure and biological function of nucleic acids (NAs) abstract NAs from their native biological context. However, cumulative experimental observations have revealed that the structure, dynamics and interactions of NAs might be strongly influenced by a broad spectrum of specific and nonspecific physical-chemical environmental factors. This consideration has recently sparked interest in the development of novel tools for structural characterization of NAs in the native cellular context. Here, we review the individual methods currently being employed for structural characterization of NA structure in a native cellular environment with a focus on recent advances and developments in the emerging fields of in-cell NMR and electron paramagnetic resonance spectroscopy and in-cell single-molecule FRET of NAs.

Links

GA16-10504S, research and development project
Name: Charakterizace struktury nukleových kyselin v komplexním prostředí živých buněk pomocí vysoce rozlišené NMR spektroskopie
Investor: Czech Science Foundation
NV15-33232A, research and development project
Name: Identifikace nových možností léčby achondroplásie prostřednictvím analýzy interakce FGFR3 a adaptérového proteinu Frs2
NV15-34405A, research and development project
Name: Identifikace nových možností léčby chronické myeloidní leukémie pomocí systematické analýzy interaktomu proteinu BCR-ABL
653706, interní kód MU
Name: iNEXT - Infrastructure for NMR, EM and X-ray crystallography for translational research (Acronym: iNEXT)
Investor: European Union, iNEXT - Infrastructure for NMR, EM and X-ray crystallography for translational research, RI Research Infrastructures (Excellent Science)