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@article{1503356,
author = {Kunka, Antonín and Damborský, Jiří and Prokop, Zbyněk},
article_location = {SAN DIEGO},
article_number = {1},
doi = {http://dx.doi.org/10.1016/bs.mie.2018.03.005},
keywords = {BIOCHEMICAL-CHARACTERIZATION; GAMMA-HEXACHLOROCYCLOHEXANE; BRADYRHIZOBIUM-JAPONICUM; DEGRADING BACTERIUM; EXPERIMENTAL-DESIGN; COLORIMETRIC ASSAY; PROTEIN STABILITY; ACCESS TUNNELS; STEADY-STATE; GEN. NOV.},
language = {eng},
issn = {0076-6879},
journal = {MARINE ENZYMES AND SPECIALIZED METABOLISM, PT B},
title = {Haloalkane Dehalogenases From Marine Organisms},
url = {http://dx.doi.org/10.1016/bs.mie.2018.03.005},
volume = {605},
year = {2018}
}
TY - JOUR
ID - 1503356
AU - Kunka, Antonín - Damborský, Jiří - Prokop, Zbyněk
PY - 2018
TI - Haloalkane Dehalogenases From Marine Organisms
JF - MARINE ENZYMES AND SPECIALIZED METABOLISM, PT B
VL - 605
IS - 1
SP - 203-251
EP - 203-251
PB - ELSEVIER ACADEMIC PRESS INC
SN - 00766879
KW - BIOCHEMICAL-CHARACTERIZATION
KW - GAMMA-HEXACHLOROCYCLOHEXANE
KW - BRADYRHIZOBIUM-JAPONICUM
KW - DEGRADING BACTERIUM
KW - EXPERIMENTAL-DESIGN
KW - COLORIMETRIC ASSAY
KW - PROTEIN STABILITY
KW - ACCESS TUNNELS
KW - STEADY-STATE
KW - GEN. NOV.
UR - http://dx.doi.org/10.1016/bs.mie.2018.03.005
N2 - Haloalkane dehalogenases degrade halogenated compounds to corresponding alcohols by a hydrolytic mechanism. These enzymes are being intensively investigated as model systems in experimental and in silico studies of enzyme mechanism and evolution, but also hold importance as useful biocatalysts for a number of biotechnological applications. Haloalkane dehalogenases originate from various organisms including bacteria (degraders, symbionts, or pathogens), eukaryotes, and archaea. Several members of this enzyme family have been found in marine organisms. The marine environment represents a good source of enzymes with novel properties, because of its diverse living conditions. A number of novel dehalogenases isolated from marine environments show interesting characteristics such as high activity, unusually broad substrate specificity, stability, or selectivity. In this chapter, the overview of haloalkane dehalogenases from marine organisms is presented and their characteristics are summarized together with an overview of the methods for their identification and biochemical characterization.
ER -
KUNKA, Antonín, Jiří DAMBORSKÝ and Zbyněk PROKOP. Haloalkane Dehalogenases From Marine Organisms. \textit{MARINE ENZYMES AND SPECIALIZED METABOLISM, PT B}. SAN DIEGO: ELSEVIER ACADEMIC PRESS INC, 2018, vol.~605, No~1, p.~203-251. ISSN~0076-6879. Available from: https://dx.doi.org/10.1016/bs.mie.2018.03.005.
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