Detailed Information on Publication Record
2019
Study of lectins from opportunistic pathogens and their interaction with normal human cells.
NOTOVÁ, Simona, Veronika SEDLÁKOVÁ, Michaela KLOUČKOVÁ, Marek KORSÁK, Martin KOLLÁR et. al.Basic information
Original name
Study of lectins from opportunistic pathogens and their interaction with normal human cells.
Authors
NOTOVÁ, Simona (703 Slovakia, belonging to the institution), Veronika SEDLÁKOVÁ (203 Czech Republic, belonging to the institution), Michaela KLOUČKOVÁ (203 Czech Republic, belonging to the institution), Marek KORSÁK (703 Slovakia, belonging to the institution), Martin KOLLÁR (703 Slovakia, belonging to the institution), Aleš HAMPL (203 Czech Republic, belonging to the institution) and Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution)
Edition
GlycoBioTec2019, 2019
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10600 1.6 Biological sciences
Country of publisher
Germany
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14740/19:00107308
Organization unit
Central European Institute of Technology
Keywords (in Czech)
lektiny; lidské buňky; fluorescenční mikroskopie
Keywords in English
lectins; human cells; fluorescence microscopy
Tags
Tags
International impact
Změněno: 11/3/2020 18:09, Mgr. Pavla Foltynová, Ph.D.
Abstract
V originále
Lectins are carbohydrate-binding proteins, highly specific for sugar moieties. They can be involved in the attachment of bacteria and viruses on their host epithelial surfaces, which is one of the most critical step leading to infection. Since the antibiotic strategy is not fully efficient anymore, new ways to cure bacterial infections are under development. One of the possibilities is anti-adhesion/anti-lectin therapy where the lectin can be inhibited by its ligand. For bringing this therapy to the medicine, first we need to identify a binding partner of the lectin and then to provide inhibition tests which approve specificity of lectins. In this work we studied interactions between lectins from Burkholderia cenocepacia and Photorhabdus luminescens, with several different types of normal non-cancerous human cells. Both lectins are fucose specific and calcium independent. The interaction was detected by fluorescence microscopy and for each observed interaction we provided inhibition tests. The lectin from B. cenocepacia interacted with surface glycoproteins of embryonal stem cells and early lung epithelial progenitors but did not bind any ligand on the surface of neural stem cells. In contrary, the lectin from P. luminescens interacted only with mesenchymal stromal cells. The obtained results may bring new promising possibilities to the research and medicine.
Links
| GA18-18964S, research and development project |
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| LQ1601, research and development project |
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