| BEDNAŘÍK, Antonín, Markéta MACHÁLKOVÁ, Eugene MOSKOVETS, Kateřina COUFALÍKOVÁ, Pavel KRÁSENSKÝ, Pavel HOUŠKA, Jiří KROUPA, Jarmila NAVRÁTILOVÁ, Jan ŠMARDA and Jan PREISLER. MALDI MS Imaging at Acquisition Rates Exceeding 100 Pixels per Second. Journal of the American Society for Mass Spectrometry. New York: Springer, 2019, vol. 30, No 2, p. 289-298. ISSN 1044-0305. Available from: https://dx.doi.org/10.1007/s13361-018-2078-8. |
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@article{1504977,
author = {Bednařík, Antonín and Machálková, Markéta and Moskovets, Eugene and Coufalíková, Kateřina and Krásenský, Pavel and Houška, Pavel and Kroupa, Jiří and Navrátilová, Jarmila and Šmarda, Jan and Preisler, Jan},
article_location = {New York},
article_number = {2},
doi = {http://dx.doi.org/10.1007/s13361-018-2078-8},
keywords = {Mass spectrometry imaging; Laser beam scanning; MALDI; TOF; MSI; High throughput; Grid ion source; 3D cell aggregates; Spheroids; Colorectal adenocarcinoma},
language = {eng},
issn = {1044-0305},
journal = {Journal of the American Society for Mass Spectrometry},
title = {MALDI MS Imaging at Acquisition Rates Exceeding 100 Pixels per Second},
url = {https://link.springer.com/article/10.1007/s13361-018-2078-8},
volume = {30},
year = {2019}
}
TY - JOUR
ID - 1504977
AU - Bednařík, Antonín - Machálková, Markéta - Moskovets, Eugene - Coufalíková, Kateřina - Krásenský, Pavel - Houška, Pavel - Kroupa, Jiří - Navrátilová, Jarmila - Šmarda, Jan - Preisler, Jan
PY - 2019
TI - MALDI MS Imaging at Acquisition Rates Exceeding 100 Pixels per Second
JF - Journal of the American Society for Mass Spectrometry
VL - 30
IS - 2
SP - 289-298
EP - 289-298
PB - Springer
SN - 10440305
KW - Mass spectrometry imaging
KW - Laser beam scanning
KW - MALDI
KW - TOF
KW - MSI
KW - High throughput
KW - Grid ion source
KW - 3D cell aggregates
KW - Spheroids
KW - Colorectal adenocarcinoma
UR - https://link.springer.com/article/10.1007/s13361-018-2078-8
L2 - https://link.springer.com/article/10.1007/s13361-018-2078-8
N2 - The practicality of matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) applied to molecular imaging of biological tissues is limited by the analysis speed. Typically, a relatively low speed of stop-and-go micromotion of XY stages is considered as a factor substantially reducing the rate with which fresh sample material can be supplied to the laser spot. The sample scan rate in our laboratory-built high-throughput imaging TOF mass spectrometer was significantly improved through the use of a galvanometer-based optical scanner performing fast laser spot repositioning on a target plate. The optical system incorporated into the ion source of our MALDI TOF mass spectrometer allowed focusing the laser beam via a modified grid into a 10-m round spot. This permitted the acquisition of high-resolution MS images with a well-defined pixel size at acquisition rates exceeding 100pixel/s. The influence of selected parameters on the total MS imaging time is discussed. The new scanning technique was employed to display the distribution of an antitumor agent in 3D colorectal adenocarcinoma cell aggregates; a single MS image comprising 100x100pixels with 10-m lateral resolution was recorded in approximately 70s.
ER -
BEDNAŘÍK, Antonín, Markéta MACHÁLKOVÁ, Eugene MOSKOVETS, Kateřina COUFALÍKOVÁ, Pavel KRÁSENSKÝ, Pavel HOUŠKA, Jiří KROUPA, Jarmila NAVRÁTILOVÁ, Jan ŠMARDA and Jan PREISLER. MALDI MS Imaging at Acquisition Rates Exceeding 100 Pixels per Second. \textit{Journal of the American Society for Mass Spectrometry}. New York: Springer, 2019, vol.~30, No~2, p.~289-298. ISSN~1044-0305. Available from: https://dx.doi.org/10.1007/s13361-018-2078-8.
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