MALDI MS Imaging at Acquisition Rates Exceeding 100 Pixels per
Second

J 2019

MALDI MS Imaging at Acquisition Rates Exceeding 100 Pixels per Second

BEDNAŘÍK, Antonín, Markéta MACHÁLKOVÁ, Eugene MOSKOVETS, Kateřina COUFALÍKOVÁ, Pavel KRÁSENSKÝ et. al.

Basic information

Original name

MALDI MS Imaging at Acquisition Rates Exceeding 100 Pixels per Second

Authors

BEDNAŘÍK, Antonín (203 Czech Republic, belonging to the institution), Markéta MACHÁLKOVÁ (203 Czech Republic, belonging to the institution), Eugene MOSKOVETS (840 United States of America), Kateřina COUFALÍKOVÁ (203 Czech Republic, belonging to the institution), Pavel KRÁSENSKÝ (203 Czech Republic, belonging to the institution), Pavel HOUŠKA (203 Czech Republic), Jiří KROUPA (203 Czech Republic), Jarmila NAVRÁTILOVÁ (203 Czech Republic, belonging to the institution), Jan ŠMARDA (203 Czech Republic, belonging to the institution) and Jan PREISLER (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of the American Society for Mass Spectrometry, New York, Springer, 2019, 1044-0305

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Full Text

Impact factor

Impact factor: 3.255

RIV identification code

RIV/00216224:14310/19:00107314

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1007/s13361-018-2078-8

UT WoS

000457210600011

Keywords in English

Mass spectrometry imaging; Laser beam scanning; MALDI; TOF; MSI; High throughput; Grid ion source; 3D cell aggregates; Spheroids; Colorectal adenocarcinoma

Abstract

V originále

The practicality of matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) applied to molecular imaging of biological tissues is limited by the analysis speed. Typically, a relatively low speed of stop-and-go micromotion of XY stages is considered as a factor substantially reducing the rate with which fresh sample material can be supplied to the laser spot. The sample scan rate in our laboratory-built high-throughput imaging TOF mass spectrometer was significantly improved through the use of a galvanometer-based optical scanner performing fast laser spot repositioning on a target plate. The optical system incorporated into the ion source of our MALDI TOF mass spectrometer allowed focusing the laser beam via a modified grid into a 10-m round spot. This permitted the acquisition of high-resolution MS images with a well-defined pixel size at acquisition rates exceeding 100pixel/s. The influence of selected parameters on the total MS imaging time is discussed. The new scanning technique was employed to display the distribution of an antitumor agent in 3D colorectal adenocarcinoma cell aggregates; a single MS image comprising 100x100pixels with 10-m lateral resolution was recorded in approximately 70s.

Links

GA15-05387S, research and development project

Name: Laserově desorpční hmotnostní spektrometrie pro analýzu molekul a nanočástic

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/G/0974/2016, interní kód MU

Name: Optimalizace cílené terapie kolorektálního karcinomu ovlivňováním účinnosti penetrace a cytotoxicity regulátorů buněčných signalizací

Investor: Masaryk University, INTERDISCIPLINARY - Interdisciplinary research projects

Citovat

BEDNAŘÍK, Antonín, Markéta MACHÁLKOVÁ, Eugene MOSKOVETS, Kateřina COUFALÍKOVÁ, Pavel KRÁSENSKÝ, Pavel HOUŠKA, Jiří KROUPA, Jarmila NAVRÁTILOVÁ, Jan ŠMARDA and Jan PREISLER. MALDI MS Imaging at Acquisition Rates Exceeding 100 Pixels per Second. Journal of the American Society for Mass Spectrometry. New York: Springer, 2019, vol. 30, No 2, p. 289-298. ISSN 1044-0305. Available from: https://dx.doi.org/10.1007/s13361-018-2078-8.

@article{1504977,
   author = {Bednařík, Antonín and Machálková, Markéta and Moskovets, Eugene and Coufalíková, Kateřina and Krásenský, Pavel and Houška, Pavel and Kroupa, Jiří and Navrátilová, Jarmila and Šmarda, Jan and Preisler, Jan},
   article_location = {New York},
   article_number = {2},
   doi = {http://dx.doi.org/10.1007/s13361-018-2078-8},
   keywords = {Mass spectrometry imaging; Laser beam scanning; MALDI; TOF; MSI; High throughput; Grid ion source; 3D cell aggregates; Spheroids; Colorectal adenocarcinoma},
   language = {eng},
   issn = {1044-0305},
   journal = {Journal of the American Society for Mass Spectrometry},
   title = {MALDI MS Imaging at Acquisition Rates Exceeding 100 Pixels per Second},
   url = {https://link.springer.com/article/10.1007/s13361-018-2078-8},
   volume = {30},
   year = {2019}
}

TY  - JOUR
ID  - 1504977
AU  - Bednařík, Antonín - Machálková, Markéta - Moskovets, Eugene - Coufalíková, Kateřina - Krásenský, Pavel - Houška, Pavel - Kroupa, Jiří - Navrátilová, Jarmila - Šmarda, Jan - Preisler, Jan
PY  - 2019
TI  - MALDI MS Imaging at Acquisition Rates Exceeding 100 Pixels per Second
JF  - Journal of the American Society for Mass Spectrometry
VL  - 30
IS  - 2
SP  - 289-298
EP  - 289-298
PB  - Springer
SN  - 10440305
KW  - Mass spectrometry imaging
KW  - Laser beam scanning
KW  - MALDI
KW  - TOF
KW  - MSI
KW  - High throughput
KW  - Grid ion source
KW  - 3D cell aggregates
KW  - Spheroids
KW  - Colorectal adenocarcinoma
UR  - https://link.springer.com/article/10.1007/s13361-018-2078-8
L2  - https://link.springer.com/article/10.1007/s13361-018-2078-8
N2  - The practicality of matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) applied to molecular imaging of biological tissues is limited by the analysis speed. Typically, a relatively low speed of stop-and-go micromotion of XY stages is considered as a factor substantially reducing the rate with which fresh sample material can be supplied to the laser spot. The sample scan rate in our laboratory-built high-throughput imaging TOF mass spectrometer was significantly improved through the use of a galvanometer-based optical scanner performing fast laser spot repositioning on a target plate. The optical system incorporated into the ion source of our MALDI TOF mass spectrometer allowed focusing the laser beam via a modified grid into a 10-m round spot. This permitted the acquisition of high-resolution MS images with a well-defined pixel size at acquisition rates exceeding 100pixel/s. The influence of selected parameters on the total MS imaging time is discussed. The new scanning technique was employed to display the distribution of an antitumor agent in 3D colorectal adenocarcinoma cell aggregates; a single MS image comprising 100x100pixels with 10-m lateral resolution was recorded in approximately 70s.
ER  -

BEDNAŘÍK, Antonín, Markéta MACHÁLKOVÁ, Eugene MOSKOVETS, Kateřina COUFALÍKOVÁ, Pavel KRÁSENSKÝ, Pavel HOUŠKA, Jiří KROUPA, Jarmila NAVRÁTILOVÁ, Jan ŠMARDA and Jan PREISLER. MALDI MS Imaging at Acquisition Rates Exceeding 100 Pixels per Second. \textit{Journal of the American Society for Mass Spectrometry}. New York: Springer, 2019, vol.~30, No~2, p.~289-298. ISSN~1044-0305. Available from: https://dx.doi.org/10.1007/s13361-018-2078-8.

Detailed Information on Publication Record