Other formats:
BibTeX
LaTeX
RIS
@article{1505317,
author = {Fan, L.G. and Zhao, L. and Hu, W. and Li, W.N. and Novak, O. and Strnad, M. and Simon, Sibu and Friml, J. and Shen, J.B. and Jiang, L.W. and Qiu, Q.S.},
article_location = {Oxford},
article_number = {4},
doi = {http://dx.doi.org/10.1111/pce.13153},
keywords = {Arabidopsis; endosomal Na+K+/H+ antiporters; AtNHX5; AtNHX6; PIN5; pH},
language = {eng},
issn = {0140-7791},
journal = {Plant, cell and environment},
title = {Na+, K+/H+ antiporters regulate the pH of endoplasmic reticulum and auxin-mediated development},
volume = {41},
year = {2018}
}
TY - JOUR
ID - 1505317
AU - Fan, L.G. - Zhao, L. - Hu, W. - Li, W.N. - Novak, O. - Strnad, M. - Simon, Sibu - Friml, J. - Shen, J.B. - Jiang, L.W. - Qiu, Q.S.
PY - 2018
TI - Na+, K+/H+ antiporters regulate the pH of endoplasmic reticulum and auxin-mediated development
JF - Plant, cell and environment
VL - 41
IS - 4
SP - 850-864
EP - 850-864
PB - Blackwell Science
SN - 01407791
KW - Arabidopsis
KW - endosomal Na+K+/H+ antiporters
KW - AtNHX5
KW - AtNHX6
KW - PIN5
KW - pH
N2 - AtNHX5 and AtNHX6 are endosomal Na+,K+/H+ antiporters that are critical for growth and development in Arabidopsis, but the mechanism behind their action remains unknown. Here, we report that AtNHX5 and AtNHX6, functioning as H+ leak, control auxin homeostasis and auxinmediated development. We found that nhx5 nhx6 exhibited growth variations of auxin-related defects. We further showed that nhx5 nhx6 was affected in auxin homeostasis. Genetic analysis showed that AtNHX5 and AtNHX6 were required for the function of the endoplasmic reticulum (ER)-localized auxin transporter PIN5. Although AtNHX5 and AtNHX6 were colocalized with PIN5 at ER, they did not interact directly. Instead, the conserved acidic residues in AtNHX5 and AtNHX6, which are essential for exchange activity, were required for PIN5 function. AtNHX5 and AtNHX6 regulated the pH in ER. Overall, AtNHX5 and AtNHX6 may regulate auxin transport across the ER via the pH gradient created by their transport activity. H+-leak pathway provides a fine-tuning mechanism that controls cellular auxin fluxes.
ER -
FAN, L.G., L. ZHAO, W. HU, W.N. LI, O. NOVAK, M. STRNAD, Sibu SIMON, J. FRIML, J.B. SHEN, L.W. JIANG and Q.S. QIU. Na+, K+/H+ antiporters regulate the pH of endoplasmic reticulum and auxin-mediated development. \textit{Plant, cell and environment}. Oxford: Blackwell Science, 2018, vol.~41, No~4, p.~850-864. ISSN~0140-7791. Available from: https://dx.doi.org/10.1111/pce.13153.
|
