Detailed Information on Publication Record
2018
The Structural Properties in Solution of the Intrinsically Mixed Folded Protein Ataxin-3
SICORELLO, A., G. KELLY, A. OREGIONI, Jiří NOVÁČEK, Vladimír SKLENÁŘ et. al.Basic information
Original name
The Structural Properties in Solution of the Intrinsically Mixed Folded Protein Ataxin-3
Authors
SICORELLO, A. (826 United Kingdom of Great Britain and Northern Ireland), G. KELLY (826 United Kingdom of Great Britain and Northern Ireland), A. OREGIONI (826 United Kingdom of Great Britain and Northern Ireland), Jiří NOVÁČEK (203 Czech Republic, guarantor, belonging to the institution), Vladimír SKLENÁŘ (203 Czech Republic, belonging to the institution) and A. PASTORE (380 Italy)
Edition
Biophysical Journal, Bethesda, USA, Biophysical Society, 2018, 0006-3495
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10608 Biochemistry and molecular biology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 3.665
RIV identification code
RIV/00216224:14740/18:00106667
Organization unit
Central European Institute of Technology
UT WoS
000437825800008
Keywords in English
EXPANDED POLYGLUTAMINE PROTEIN; LINEAR LATTICE MODEL; EXON-1 N-TERMINUS; JOSEPHIN DOMAIN; SECONDARY STRUCTURE; NMR-SPECTROSCOPY; DISORDERED PROTEINS; UBIQUITIN-BINDING; FOURIER-TRANSFORM; DISEASE PROTEIN
Tags
International impact, Reviewed
Změněno: 19/3/2019 16:30, Mgr. Pavla Foltynová, Ph.D.
Abstract
V originále
It has increasingly become clear over the last two decades that proteins can contain both globular domains and intrinsically unfolded regions that can both contribute to function. Although equally interesting, the disordered regions are difficult to study, because they usually do not crystallize unless bound to partners and are not easily amenable to cryo-electron microscopy studies. NMR spectroscopy remains the best technique to capture the structural features of intrinsically mixed folded proteins and describe their dynamics. These studies rely on the successful assignment of the spectrum, a task not easy per se given the limited spread of the resonances of the disordered residues. Here, we describe the structural properties of ataxin-3, the protein responsible for the neurodegenerative Machado-Joseph disease. Ataxin-3 is a 42-kDa protein containing a globular N-terminal Josephin domain and a C-terminal tail that comprises 13 polyglutamine repeats within a low complexity region. We developed a strategy that allowed us to achieve 87% assignment of the NMR spectrum using a mixed protocol based on high-dimensionality, high-resolution experiments and different labeling schemes. Thanks to the almost complete spectral assignment, we proved that the C-terminal tail is flexible, with extended helical regions, and interacts only marginally with the rest of the protein. We could also, for the first time to our knowledge, observe the structural propensity of the polyglutamine repeats within the context of the full-length protein and show that its structure is stabilized by the preceding region.
Links
| LM2015043, research and development project |
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