Letter to the Editor concerning

J 2018

Letter to the Editor concerning "Ribosomal PCR assay of excised intervertebral discs from patients undergoing single-level primary lumbar microdiscectomy.'' by Alamin TF, Munoz M, Zagel A, et al.: Eur Spine J 2017

CAPOOR, Manu, P. LAMBERT and Ondřej SLABÝ

Basic information

Original name

Letter to the Editor concerning "Ribosomal PCR assay of excised intervertebral discs from patients undergoing single-level primary lumbar microdiscectomy.'' by Alamin TF, Munoz M, Zagel A, et al.: Eur Spine J 2017

Authors

CAPOOR, Manu (840 United States of America, belonging to the institution), P. LAMBERT (826 United Kingdom of Great Britain and Northern Ireland) and Ondřej SLABÝ (203 Czech Republic, guarantor, belonging to the institution)

Edition

European Spine Journal, NEW YORK, Springer, 2018, 0940-6719

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30210 Clinical neurology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.513

RIV identification code

RIV/00216224:14740/18:00106681

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1007/s00586-017-5379-x

UT WoS

000425292500037

Keywords in English

Diskectomy; Humans; Intervertebral Disc; Lumbar Vertebrae; Polymerase Chain Reaction

Abstract

V originále

Based on epidemiological evidence (Urquhart et al. 2017), direct microscopic evidence of Propionibacterium acnes (P. acnes) biofilm in degenerated disc tissue (Capoor et al. 2017), and experimental induction of degenerative disc disease in an animal model using P. acnes (Shan et al. 2017), among other evidence, P. acnes infection is emerging as an etiological factor in degenerative disc disease. Therefore, a recent article published by Alamin et al. in European Spine Journal has raised some concern (Alamin et al. 2017). Alamin et al. failed to find evidence of any bacterial DNA in disc tissue obtained from 44 patients with radiculopathy and MRI findings of lumbar herniated nucleus pulposus who underwent lumbar microdiscectomy. They employed a PCR/amplicon sequencing assay used for the routine diagnosis of invasive infections and declared this method to detect 97.7% of infected tissues and fluid samples, using culture as the reference method (unpublished data).

Citovat

CAPOOR, Manu, P. LAMBERT and Ondřej SLABÝ. Letter to the Editor concerning "Ribosomal PCR assay of excised intervertebral discs from patients undergoing single-level primary lumbar microdiscectomy.'' by Alamin TF, Munoz M, Zagel A, et al.: Eur Spine J 2017. European Spine Journal. NEW YORK: Springer, 2018, vol. 27, No 2, p. 516-517. ISSN 0940-6719. Available from: https://dx.doi.org/10.1007/s00586-017-5379-x.

@article{1508678,
   author = {Capoor, Manu and Lambert, P. and Slabý, Ondřej},
   article_location = {NEW YORK},
   article_number = {2},
   doi = {http://dx.doi.org/10.1007/s00586-017-5379-x},
   keywords = {Diskectomy; Humans; Intervertebral Disc; Lumbar Vertebrae; Polymerase Chain Reaction},
   language = {eng},
   issn = {0940-6719},
   journal = {European Spine Journal},
   title = {Letter to the Editor concerning "Ribosomal PCR assay of excised intervertebral discs from patients undergoing single-level primary lumbar microdiscectomy.'' by Alamin TF, Munoz M, Zagel A, et al.: Eur Spine J 2017},
   volume = {27},
   year = {2018}
}

TY  - JOUR
ID  - 1508678
AU  - Capoor, Manu - Lambert, P. - Slabý, Ondřej
PY  - 2018
TI  - Letter to the Editor concerning "Ribosomal PCR assay of excised intervertebral discs from patients undergoing single-level primary lumbar microdiscectomy.'' by Alamin TF, Munoz M, Zagel A, et al.: Eur Spine J 2017
JF  - European Spine Journal
VL  - 27
IS  - 2
SP  - 516-517
EP  - 516-517
PB  - Springer
SN  - 09406719
KW  - Diskectomy
KW  - Humans
KW  - Intervertebral Disc
KW  - Lumbar Vertebrae
KW  - Polymerase Chain Reaction
N2  - Based on epidemiological evidence (Urquhart et al. 2017), direct microscopic evidence of Propionibacterium acnes (P. acnes) biofilm in degenerated disc tissue (Capoor et al. 2017), and experimental induction of degenerative disc disease in an animal model using P. acnes (Shan et al. 2017), among other evidence, P. acnes infection is emerging as an etiological factor in degenerative disc disease. Therefore, a recent article published by Alamin et al. in European Spine Journal has raised some concern (Alamin et al. 2017). Alamin et al. failed to find evidence of any bacterial DNA in disc tissue obtained from 44 patients with radiculopathy and MRI findings of lumbar herniated nucleus pulposus who underwent lumbar microdiscectomy. They employed a PCR/amplicon sequencing assay used for the routine diagnosis of invasive infections and declared this method to detect 97.7% of infected tissues and fluid samples, using culture as the reference method (unpublished data).
ER  -

CAPOOR, Manu, P. LAMBERT and Ondřej SLABÝ. Letter to the Editor concerning ''Ribosomal PCR assay of excised intervertebral discs from patients undergoing single-level primary lumbar microdiscectomy.'' by Alamin TF, Munoz M, Zagel A, et al.: Eur Spine J 2017. \textit{European Spine Journal}. NEW YORK: Springer, 2018, vol.~27, No~2, p.~516-517. ISSN~0940-6719. Available from: https://dx.doi.org/10.1007/s00586-017-5379-x.

Detailed Information on Publication Record