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@proceedings{1512379,
author = {Tvrdoňová, Michaela and Ascher, Lena and Jakubowski, Norbert and Vlčnovská, Marcela and Vaculovičová, Markéta and Kanický, Viktor and Vaculovič, Tomáš},
booktitle = {European Symposium on Atomic Spectrometry},
keywords = {antibody labelling; LA-ICP-MS; Au nanoparticles; imaging},
language = {eng},
title = {A new approach of antibodies labeling (NPs) by immunoassay with LA-ICP-MS detection},
year = {2018}
}
TY - CONF
ID - 1512379
AU - Tvrdoňová, Michaela - Ascher, Lena - Jakubowski, Norbert - Vlčnovská, Marcela - Vaculovičová, Markéta - Kanický, Viktor - Vaculovič, Tomáš
PY - 2018
TI - A new approach of antibodies labeling (NPs) by immunoassay with LA-ICP-MS detection
KW - antibody labelling
KW - LA-ICP-MS
KW - Au nanoparticles
KW - imaging
N2 - Laser ablation with inductively coupled plasma is still more used in life science as biology and biomedicine and the utilization of metals and proteins determination simultaneously is also growing up. 1,2 We have developed a new strategy of antibody labeling (it can specific binds to proteins) by Au nanoparticles which are composed of thousands of atoms and thus increase the sensitivity enormously and decrease the limit of detection in comparison to lanthanoids labeling. The ability of successfully labelled antibodies bounded to antigen (protein) was proved by dot blot on the membrane and the Au was imaged by LA-ICP-MS. One of the many possible applications is binding of labelled antibodies to the tumour tissues containing metallothionein (known as a transporter of Cu, Zn and heavy metals) and image their distribution in different tumour stage.
ER -
TVRDOŇOVÁ, Michaela, Lena ASCHER, Norbert JAKUBOWSKI, Marcela VLČNOVSKÁ, Markéta VACULOVIČOVÁ, Viktor KANICKÝ a Tomáš VACULOVIČ. A new approach of antibodies labeling (NPs) by immunoassay with LA-ICP-MS detection. In \textit{European Symposium on Atomic Spectrometry}. 2018.
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