| TVRDOŇOVÁ, Michaela, Marcela VLČNOVSKÁ, Lucie VANICKOVA, Viktor KANICKÝ, Vojtěch ADAM, Lena ASCHER, Norbert JAKUBOWSKI, Markéta VACULOVIČOVÁ and Tomáš VACULOVIČ. Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry. Analytical and Bioanalytical chemistry. HEIDELBERG: SPRINGER HEIDELBERG, 2019, vol. 411, No 3, p. 559-564. ISSN 1618-2642. Available from: https://dx.doi.org/10.1007/s00216-018-1300-7. |
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@article{1512417,
author = {Tvrdoňová, Michaela and Vlčnovská, Marcela and Vanickova, Lucie and Kanický, Viktor and Adam, Vojtěch and Ascher, Lena and Jakubowski, Norbert and Vaculovičová, Markéta and Vaculovič, Tomáš},
article_location = {HEIDELBERG},
article_number = {3},
doi = {http://dx.doi.org/10.1007/s00216-018-1300-7},
keywords = {Dot blot; Antibody labelling; Gold nanoparticles; LA-ICP-MS; Immunochemistry},
language = {eng},
issn = {1618-2642},
journal = {Analytical and Bioanalytical chemistry},
title = {Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry},
url = {http://dx.doi.org/10.1007/s00216-018-1300-7},
volume = {411},
year = {2019}
}
TY - JOUR
ID - 1512417
AU - Tvrdoňová, Michaela - Vlčnovská, Marcela - Vanickova, Lucie - Kanický, Viktor - Adam, Vojtěch - Ascher, Lena - Jakubowski, Norbert - Vaculovičová, Markéta - Vaculovič, Tomáš
PY - 2019
TI - Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry
JF - Analytical and Bioanalytical chemistry
VL - 411
IS - 3
SP - 559-564
EP - 559-564
PB - SPRINGER HEIDELBERG
SN - 16182642
KW - Dot blot
KW - Antibody labelling
KW - Gold nanoparticles
KW - LA-ICP-MS
KW - Immunochemistry
UR - http://dx.doi.org/10.1007/s00216-018-1300-7
L2 - http://dx.doi.org/10.1007/s00216-018-1300-7
N2 - In this paper, we describe the labelling of antibodies by gold nanoparticles (AuNPs) with diameters of 10 and 60nm with detection by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Additionally, the AuNPs labelling strategy is compared with commercially available labelling reagents based on MeCAT (metal coded affinity tagging). Proof of principle experiments based on dot blot experiments were performed. The two labelling methods investigated were compared by sensitivity and limit of detection (LOD). The absolute LODs achieved were in the range of tens of picograms for AuNP labelling compared to a few hundred picograms by the MeCAT labelling.
ER -
TVRDOŇOVÁ, Michaela, Marcela VLČNOVSKÁ, Lucie VANICKOVA, Viktor KANICKÝ, Vojtěch ADAM, Lena ASCHER, Norbert JAKUBOWSKI, Markéta VACULOVIČOVÁ and Tomáš VACULOVIČ. Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry. \textit{Analytical and Bioanalytical chemistry}. HEIDELBERG: SPRINGER HEIDELBERG, 2019, vol.~411, No~3, p.~559-564. ISSN~1618-2642. Available from: https://dx.doi.org/10.1007/s00216-018-1300-7.
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