Gold nanoparticles as labels for immunochemical analysis using
laser ablation inductively coupled plasma mass spectrometry

J 2019

Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry

TVRDOŇOVÁ, Michaela, Marcela VLČNOVSKÁ, Lucie VANICKOVA, Viktor KANICKÝ, Vojtěch ADAM et. al.

Basic information

Original name

Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry

Authors

TVRDOŇOVÁ, Michaela (203 Czech Republic, belonging to the institution), Marcela VLČNOVSKÁ (203 Czech Republic), Lucie VANICKOVA (203 Czech Republic), Viktor KANICKÝ (203 Czech Republic, belonging to the institution), Vojtěch ADAM (203 Czech Republic), Lena ASCHER (276 Germany), Norbert JAKUBOWSKI (276 Germany), Markéta VACULOVIČOVÁ (203 Czech Republic) and Tomáš VACULOVIČ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Analytical and Bioanalytical chemistry, HEIDELBERG, SPRINGER HEIDELBERG, 2019, 1618-2642

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.637

RIV identification code

RIV/00216224:14310/19:00107331

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1007/s00216-018-1300-7

UT WoS

000456132700003

Keywords in English

Dot blot; Antibody labelling; Gold nanoparticles; LA-ICP-MS; Immunochemistry

Abstract

V originále

In this paper, we describe the labelling of antibodies by gold nanoparticles (AuNPs) with diameters of 10 and 60nm with detection by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Additionally, the AuNPs labelling strategy is compared with commercially available labelling reagents based on MeCAT (metal coded affinity tagging). Proof of principle experiments based on dot blot experiments were performed. The two labelling methods investigated were compared by sensitivity and limit of detection (LOD). The absolute LODs achieved were in the range of tens of picograms for AuNP labelling compared to a few hundred picograms by the MeCAT labelling.

Links

GA17-12774S, research and development project

Name: Využití vícenásobného značení pomocí kovových nanočástic pro bio-zobrazování

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

TVRDOŇOVÁ, Michaela, Marcela VLČNOVSKÁ, Lucie VANICKOVA, Viktor KANICKÝ, Vojtěch ADAM, Lena ASCHER, Norbert JAKUBOWSKI, Markéta VACULOVIČOVÁ and Tomáš VACULOVIČ. Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry. Analytical and Bioanalytical chemistry. HEIDELBERG: SPRINGER HEIDELBERG, 2019, vol. 411, No 3, p. 559-564. ISSN 1618-2642. Available from: https://dx.doi.org/10.1007/s00216-018-1300-7.

@article{1512417,
   author = {Tvrdoňová, Michaela and Vlčnovská, Marcela and Vanickova, Lucie and Kanický, Viktor and Adam, Vojtěch and Ascher, Lena and Jakubowski, Norbert and Vaculovičová, Markéta and Vaculovič, Tomáš},
   article_location = {HEIDELBERG},
   article_number = {3},
   doi = {http://dx.doi.org/10.1007/s00216-018-1300-7},
   keywords = {Dot blot; Antibody labelling; Gold nanoparticles; LA-ICP-MS; Immunochemistry},
   language = {eng},
   issn = {1618-2642},
   journal = {Analytical and Bioanalytical chemistry},
   title = {Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry},
   url = {http://dx.doi.org/10.1007/s00216-018-1300-7},
   volume = {411},
   year = {2019}
}

TY  - JOUR
ID  - 1512417
AU  - Tvrdoňová, Michaela - Vlčnovská, Marcela - Vanickova, Lucie - Kanický, Viktor - Adam, Vojtěch - Ascher, Lena - Jakubowski, Norbert - Vaculovičová, Markéta - Vaculovič, Tomáš
PY  - 2019
TI  - Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry
JF  - Analytical and Bioanalytical chemistry
VL  - 411
IS  - 3
SP  - 559-564
EP  - 559-564
PB  - SPRINGER HEIDELBERG
SN  - 16182642
KW  - Dot blot
KW  - Antibody labelling
KW  - Gold nanoparticles
KW  - LA-ICP-MS
KW  - Immunochemistry
UR  - http://dx.doi.org/10.1007/s00216-018-1300-7
L2  - http://dx.doi.org/10.1007/s00216-018-1300-7
N2  - In this paper, we describe the labelling of antibodies by gold nanoparticles (AuNPs) with diameters of 10 and 60nm with detection by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Additionally, the AuNPs labelling strategy is compared with commercially available labelling reagents based on MeCAT (metal coded affinity tagging). Proof of principle experiments based on dot blot experiments were performed. The two labelling methods investigated were compared by sensitivity and limit of detection (LOD). The absolute LODs achieved were in the range of tens of picograms for AuNP labelling compared to a few hundred picograms by the MeCAT labelling.
ER  -

TVRDOŇOVÁ, Michaela, Marcela VLČNOVSKÁ, Lucie VANICKOVA, Viktor KANICKÝ, Vojtěch ADAM, Lena ASCHER, Norbert JAKUBOWSKI, Markéta VACULOVIČOVÁ and Tomáš VACULOVIČ. Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry. \textit{Analytical and Bioanalytical chemistry}. HEIDELBERG: SPRINGER HEIDELBERG, 2019, vol.~411, No~3, p.~559-564. ISSN~1618-2642. Available from: https://dx.doi.org/10.1007/s00216-018-1300-7.

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