SUPPRESION OF PROTEIN SAMPLE LOSSES IN AUTOSAMPLER VIALS

J 2013

SUPPRESION OF PROTEIN SAMPLE LOSSES IN AUTOSAMPLER VIALS

STEJSKAL, Karel, David POTĚŠIL and Zbyněk ZDRÁHAL

Basic information

Original name

SUPPRESION OF PROTEIN SAMPLE LOSSES IN AUTOSAMPLER VIALS

Authors

STEJSKAL, Karel (203 Czech Republic, belonging to the institution), David POTĚŠIL (203 Czech Republic, belonging to the institution) and Zbyněk ZDRÁHAL (203 Czech Republic, guarantor, belonging to the institution)

Edition

CHEMICKÉ LISTY, Praha, Česká společnost chemická, 2013, 0009-2770

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.196

RIV identification code

RIV/00216224:14740/13:00107015

Organization unit

Central European Institute of Technology

UT WoS

000328730800017

Keywords in English

RECOVERY; PEPTIDES

Abstract

V originále

In the present study, we assessed peptide losses during sample storage in autosampler vials. We evaluated the possibility to suppress the adsorption effects by changing the composition of the sample solution injected into the liquid chromatography coupled with tandem mass spectrometry system (LC-MS/MS) and by use of different material of autosampler vials. As a model sample, a tryptic digest of six bovine proteins (B6E) in the amount of 1 fmol per protein was used for comparative experiments. The combination of a polypropylene vial and solution of poly(ethylene glycol) (PEG) (0.001%) or a mixture of high concentrated urea and thiourea (5M and 1M) as injection solutions provided the best results in terms of number of significantly identified peptides (P < 0.05). These conclusions were confirmed by analyses of a real sample with intermediate complexity. Addition of PEG into the real sample solution proved to prevent higher losses, concerning mainly hydrophobic peptides, during up to 48 h storage in the autosampler in comparison with a formic acid solution and even with a solution of highly concentrated urea and thiourea(1).

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

GBP206/12/G151, research and development project

Name: Centrum nových přístupů k bioanalýze a molekulární diagnostice

Citovat

STEJSKAL, Karel, David POTĚŠIL and Zbyněk ZDRÁHAL. SUPPRESION OF PROTEIN SAMPLE LOSSES IN AUTOSAMPLER VIALS. CHEMICKÉ LISTY. Praha: Česká společnost chemická, 2013, vol. 107, No 2013, p. "S324"-"S326", 3 pp. ISSN 0009-2770.

@article{1512558,
   author = {Stejskal, Karel and Potěšil, David and Zdráhal, Zbyněk},
   article_location = {Praha},
   article_number = {2013},
   keywords = {RECOVERY; PEPTIDES},
   language = {eng},
   issn = {0009-2770},
   journal = {CHEMICKÉ LISTY},
   title = {SUPPRESION OF PROTEIN SAMPLE LOSSES IN AUTOSAMPLER VIALS},
   volume = {107},
   year = {2013}
}

TY  - JOUR
ID  - 1512558
AU  - Stejskal, Karel - Potěšil, David - Zdráhal, Zbyněk
PY  - 2013
TI  - SUPPRESION OF PROTEIN SAMPLE LOSSES IN AUTOSAMPLER VIALS
JF  - CHEMICKÉ LISTY
VL  - 107
IS  - 2013
SP  - "S324"-"S326"
EP  - "S324"-"S326"
PB  - Česká společnost chemická
SN  - 00092770
KW  - RECOVERY
KW  - PEPTIDES
N2  - In the present study, we assessed peptide losses during sample storage in autosampler vials. We evaluated the possibility to suppress the adsorption effects by changing the composition of the sample solution injected into the liquid chromatography coupled with tandem mass spectrometry system (LC-MS/MS) and by use of different material of autosampler vials. As a model sample, a tryptic digest of six bovine proteins (B6E) in the amount of 1 fmol per protein was used for comparative experiments. The combination of a polypropylene vial and solution of poly(ethylene glycol) (PEG) (0.001%) or a mixture of high concentrated urea and thiourea (5M and 1M) as injection solutions provided the best results in terms of number of significantly identified peptides (P < 0.05). These conclusions were confirmed by analyses of a real sample with intermediate complexity. Addition of PEG into the real sample solution proved to prevent higher losses, concerning mainly hydrophobic peptides, during up to 48 h storage in the autosampler in comparison with a formic acid solution and even with a solution of highly concentrated urea and thiourea(1).
ER  -

STEJSKAL, Karel, David POTĚŠIL and Zbyněk ZDRÁHAL. SUPPRESION OF PROTEIN SAMPLE LOSSES IN AUTOSAMPLER VIALS. \textit{CHEMICKÉ LISTY}. Praha: Česká společnost chemická, 2013, vol.~107, No~2013, p.~''S324''-''S326'', 3 pp. ISSN~0009-2770.

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