Antibacterial biocompatible PCL nanofibers modified by
COOH-anhydride plasma polymers and gentamicin immobilization

J 2018

Antibacterial biocompatible PCL nanofibers modified by COOH-anhydride plasma polymers and gentamicin immobilization

PERMYAKOVA, E.S., J. POLCAK, P.V. SLUKIN, S.G. IGNATOV, N.A. GLOUSHANKOVA et. al.

Basic information

Original name

Antibacterial biocompatible PCL nanofibers modified by COOH-anhydride plasma polymers and gentamicin immobilization

Authors

PERMYAKOVA, E.S. (643 Russian Federation), J. POLCAK (643 Russian Federation), P.V. SLUKIN (643 Russian Federation), S.G. IGNATOV (643 Russian Federation), N.A. GLOUSHANKOVA (643 Russian Federation), Lenka ZAJÍČKOVÁ (203 Czech Republic, guarantor, belonging to the institution), D.V. SHTANSKY (643 Russian Federation) and A. MANAKHOV (643 Russian Federation)

Edition

MATERIALS & DESIGN, OXFORD, ELSEVIER SCI LTD, 2018, 0264-1275

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10403 Physical chemistry

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 5.770

RIV identification code

RIV/00216224:14740/18:00106734

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.matdes.2018.05.002

UT WoS

000436433600007

Keywords in English

Nanofibers; Biomaterials; Cell adhesion; Antibacterial effect; Gentamicin; Plasma polymer

Abstract

V originále

Herein COOH/anhydride functionalized biodegradable nanofibers were prepared using atmospheric pressure plasma copolymerization of maleic anhydride (MA) and C2H2. The XPS and ATR-FTIR analyses revealed a high concentration of carboxyl and anhydride groups that were used for grafting of gentamycin (GM) onto the surface of PCL nanofibers. It was shown that GM can be immobilized either with or without dicyclohexyl carbodiimde (DCC). The immobilization without DCC led to ionic bonding (PCL-MA-GMi), whereas the immobilization using DCC activation resulted in covalent bonding (PCL-MA-GMc). The investigation of IAR2 epithelial cell adhesion and proliferation revealed that the GM-loaded nanofibers were biocompatible. The nanofibers without immobilized GM did not show any noticeable antibacterial activity against E. coli bacteria with different resistance to gentamicin. In contrast, the size of inhibition zone around the PCL-MA-GMi and PCL-MA-GMc samples reached 27 mm, hereby indicating a strong antibacterial effect against all types of E. coli bacteria. The GM-loaded nanofibers also demonstrated a pronounced antibacterial effect after immersion in phosphate buffered saline at 37 degrees C for 24 h. Thus the results demonstrated that the proposed strategy for the preparation of antibacterial biocompatible nanofibers with relatively long-termantibacterial protection has a great potential for future application for wound healing. (C) 2018 Elsevier Ltd. All rights reserved.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

PERMYAKOVA, E.S., J. POLCAK, P.V. SLUKIN, S.G. IGNATOV, N.A. GLOUSHANKOVA, Lenka ZAJÍČKOVÁ, D.V. SHTANSKY and A. MANAKHOV. Antibacterial biocompatible PCL nanofibers modified by COOH-anhydride plasma polymers and gentamicin immobilization. MATERIALS & DESIGN. OXFORD: ELSEVIER SCI LTD, 2018, vol. 153, SEP, p. 60-70. ISSN 0264-1275. Available from: https://dx.doi.org/10.1016/j.matdes.2018.05.002.

@article{1512757,
   author = {Permyakova, E.S. and Polcak, J. and Slukin, P.V. and Ignatov, S.G. and Gloushankova, N.A. and Zajíčková, Lenka and Shtansky, D.V. and Manakhov, A.},
   article_location = {OXFORD},
   article_number = {SEP},
   doi = {http://dx.doi.org/10.1016/j.matdes.2018.05.002},
   keywords = {Nanofibers; Biomaterials; Cell adhesion; Antibacterial effect; Gentamicin; Plasma polymer},
   language = {eng},
   issn = {0264-1275},
   journal = {MATERIALS & DESIGN},
   title = {Antibacterial biocompatible PCL nanofibers modified by COOH-anhydride plasma polymers and gentamicin immobilization},
   volume = {153},
   year = {2018}
}

TY  - JOUR
ID  - 1512757
AU  - Permyakova, E.S. - Polcak, J. - Slukin, P.V. - Ignatov, S.G. - Gloushankova, N.A. - Zajíčková, Lenka - Shtansky, D.V. - Manakhov, A.
PY  - 2018
TI  - Antibacterial biocompatible PCL nanofibers modified by COOH-anhydride plasma polymers and gentamicin immobilization
JF  - MATERIALS & DESIGN
VL  - 153
IS  - SEP
SP  - 60-70
EP  - 60-70
PB  - ELSEVIER SCI LTD
SN  - 02641275
KW  - Nanofibers
KW  - Biomaterials
KW  - Cell adhesion
KW  - Antibacterial effect
KW  - Gentamicin
KW  - Plasma polymer
N2  - Herein COOH/anhydride functionalized biodegradable nanofibers were prepared using atmospheric pressure plasma copolymerization of maleic anhydride (MA) and C2H2. The XPS and ATR-FTIR analyses revealed a high concentration of carboxyl and anhydride groups that were used for grafting of gentamycin (GM) onto the surface of PCL nanofibers. It was shown that GM can be immobilized either with or without dicyclohexyl carbodiimde (DCC). The immobilization without DCC led to ionic bonding (PCL-MA-GMi), whereas the immobilization using DCC activation resulted in covalent bonding (PCL-MA-GMc). The investigation of IAR2 epithelial cell adhesion and proliferation revealed that the GM-loaded nanofibers were biocompatible. The nanofibers without immobilized GM did not show any noticeable antibacterial activity against E. coli bacteria with different resistance to gentamicin. In contrast, the size of inhibition zone around the PCL-MA-GMi and PCL-MA-GMc samples reached 27 mm, hereby indicating a strong antibacterial effect against all types of E. coli bacteria. The GM-loaded nanofibers also demonstrated a pronounced antibacterial effect after immersion in phosphate buffered saline at 37 degrees C for 24 h. Thus the results demonstrated that the proposed strategy for the preparation of antibacterial biocompatible nanofibers with relatively long-termantibacterial protection has a great potential for future application for wound healing. (C) 2018 Elsevier Ltd. All rights reserved.
ER  -

PERMYAKOVA, E.S., J. POLCAK, P.V. SLUKIN, S.G. IGNATOV, N.A. GLOUSHANKOVA, Lenka ZAJÍČKOVÁ, D.V. SHTANSKY and A. MANAKHOV. Antibacterial biocompatible PCL nanofibers modified by COOH-anhydride plasma polymers and gentamicin immobilization. \textit{MATERIALS \&{}amp; DESIGN}. OXFORD: ELSEVIER SCI LTD, 2018, vol.~153, SEP, p.~60-70. ISSN~0264-1275. Available from: https://dx.doi.org/10.1016/j.matdes.2018.05.002.

Detailed Information on Publication Record