Detailed Information on Publication Record
2019
Diclofenac as an environmental threat: Impact on the photosynthetic processes of Lemna minor chloroplasts
HÁJKOVÁ, Markéta, Marie KUMMEROVÁ, Štěpán ZEZULKA, Petr BABULA, Peter VÁCZI et. al.Basic information
Original name
Diclofenac as an environmental threat: Impact on the photosynthetic processes of Lemna minor chloroplasts
Name in Czech
Diklofenak jako environmentální hrozba: Dopad na fotosyntetické procesy chloroplastů Lemna minor
Name (in English)
Diclofenac as an environmental threat: Impact on the photosynthetic processes of Lemna minor chloroplasts
Authors
HÁJKOVÁ, Markéta (203 Czech Republic, guarantor, belonging to the institution), Marie KUMMEROVÁ (203 Czech Republic, belonging to the institution), Štěpán ZEZULKA (203 Czech Republic, belonging to the institution), Petr BABULA (203 Czech Republic, belonging to the institution) and Peter VÁCZI (703 Slovakia, belonging to the institution)
Edition
Chemosphere, OXFORD, PERGAMON-ELSEVIER SCIENCE LTD, 2019, 0045-6535
Other information
Language
Czech
Type of outcome
Článek v odborném periodiku
Field of Study
10611 Plant sciences, botany
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 5.778
RIV identification code
RIV/00216224:14310/19:00107335
Organization unit
Faculty of Science
UT WoS
000466249600100
Keywords (in Czech)
Diklofenak; chloroplasty okřehku; fotosystémy II a I; RuBisCO; oxidativní stres;
Keywords in English
Diclofenac; Duckweed chloroplasts; Photosystems II and I; RuBisCO; Oxidative stress;
Tags
International impact, Reviewed
Změněno: 17/4/2020 16:27, Mgr. Marie Šípková, DiS.
V originále
Mechanisms of pharmaceuticals action on biochemical and physiological processes in plants that determine plant growth and development are still mostly unknown. This study deals with the effects of non-steroidal anti-inflammatory drug diclofenac (DCF) on photosynthesis as an essential anabolic process. Changes in primary and secondary photosynthetic processes were assessed in chloroplasts isolated from Lemna minor exposed to 1, 10, 100, and 1000 uM DCF. Decreases in the potential and effective quantum yields of photosystem II (FV/FM by 21%, PhiII by 44% compared to control), changes in non-photochemical fluorescence quenching (NPQ), and a substantial drop in Hill reaction activity (by 73%), especially under 1000 uM DCF, were found. Limitation of electron transport through photosystem II was confirmed by increased fluorescence signals in steps J and I (by 50% and 23%, respectively, under 1000 uM DCF) in OJIP fluorescence transient. Photosystem I exhibited changes only in the redox state of P700 reaction centres (decrease in Pm by 10%, increase in reduced P700 by 5% under 1000 uM DCF). Similarly, RuBisCO activity was only lowered by 30% under 1000 uM DCF. In contrast, a significant increase in reactive oxygen and nitrogen species (by 116% and 157%, respectively) was observed under 10 uM DCF, and lipid peroxidation increased even at 1 uM DCF (by nearly seven times compared to the control). Results demonstrate the ability of environmentally relevant DCF concentrations to induce oxidative stress in isolated duckweed chloroplasts; however, photosynthetic processes were affected considerably only by the highest DCF treatments.
In English
Mechanisms of pharmaceuticals action on biochemical and physiological processes in plants that determine plant growth and development are still mostly unknown. This study deals with the effects of non-steroidal anti-inflammatory drug diclofenac (DCF) on photosynthesis as an essential anabolic process. Changes in primary and secondary photosynthetic processes were assessed in chloroplasts isolated from Lemna minor exposed to 1, 10, 100, and 1000 uM DCF. Decreases in the potential and effective quantum yields of photosystem II (FV/FM by 21%, PhiII by 44% compared to control), changes in non-photochemical fluorescence quenching (NPQ), and a substantial drop in Hill reaction activity (by 73%), especially under 1000 uM DCF, were found. Limitation of electron transport through photosystem II was confirmed by increased fluorescence signals in steps J and I (by 50% and 23%, respectively, under 1000 uM DCF) in OJIP fluorescence transient. Photosystem I exhibited changes only in the redox state of P700 reaction centres (decrease in Pm by 10%, increase in reduced P700 by 5% under 1000 uM DCF). Similarly, RuBisCO activity was only lowered by 30% under 1000 uM DCF. In contrast, a significant increase in reactive oxygen and nitrogen species (by 116% and 157%, respectively) was observed under 10 uM DCF, and lipid peroxidation increased even at 1 uM DCF (by nearly seven times compared to the control). Results demonstrate the ability of environmentally relevant DCF concentrations to induce oxidative stress in isolated duckweed chloroplasts; however, photosynthetic processes were affected considerably only by the highest DCF treatments.
Links
| GF17-33746L, research and development project |
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