WNT5A is transported via lipoprotein particles in the
cerebrospinal fluid to regulate hindbrain morphogenesis

J 2019

WNT5A is transported via lipoprotein particles in the cerebrospinal fluid to regulate hindbrain morphogenesis

KAISER, Karol, D. GYLLBORG, J. PROCHAZKA, Alena SALAŠOVÁ, Petra KOMPANÍKOVÁ et. al.

Basic information

Original name

WNT5A is transported via lipoprotein particles in the cerebrospinal fluid to regulate hindbrain morphogenesis

Authors

KAISER, Karol (703 Slovakia, belonging to the institution), D. GYLLBORG (752 Sweden), J. PROCHAZKA (203 Czech Republic), Alena SALAŠOVÁ (203 Czech Republic), Petra KOMPANÍKOVÁ (703 Slovakia, belonging to the institution), F.L. MOLINA (724 Spain), R. LAGUNA-GOYA (826 United Kingdom of Great Britain and Northern Ireland), Tomasz Witold RADASZKIEWICZ (616 Poland, belonging to the institution), Jakub HARNOŠ (203 Czech Republic, belonging to the institution), M. PROCHAZKOVA (203 Czech Republic), David POTĚŠIL (203 Czech Republic, belonging to the institution), R.A. BARKER (826 United Kingdom of Great Britain and Northern Ireland), A.G. CASADO (724 Spain), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution), R. SEDLACEK (203 Czech Republic), E. ARENAS (752 Sweden), Juan Carlos VILLAESCUSA (724 Spain, belonging to the institution) and Vítězslav BRYJA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Nature Communications, London, Nature Publishing Group, 2019, 2041-1723

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10605 Developmental biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 12.121

RIV identification code

RIV/00216224:14310/19:00107349

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1038/s41467-019-09298-4

UT WoS

000462986000002

Keywords in English

LOW-DENSITY LIPOPROTEINS; CELLS; PROTEINS; RECEPTOR; EXPRESSION; MUTATIONS; SECRETION; PROTEOME; HEDGEHOG; SYSTEM

Abstract

V originále

WNTs are lipid-modified proteins that control multiple functions in development and disease via short- and long-range signaling. However, it is unclear how these hydrophobic molecules spread over long distances in the mammalian brain. Here we show that WNT5A is produced by the choroid plexus (ChP) of the developing hindbrain, but not the telencephalon, in both mouse and human. Since the ChP produces and secretes the cerebrospinal fluid (CSF), we examine the presence of WNT5A in the CSF and find that it is associated with lipoprotein particles rather than exosomes. Moreover, since the CSF flows along the apical surface of hindbrain progenitors not expressing Wnt5a, we examined whether deletion of Wnt5a in the ChP controls their function and find that cerebellar morphogenesis is impaired. Our study thus identifies the CSF as a route and lipoprotein particles as a vehicle for long-range transport of biologically active WNT in the central nervous system.

Links

EE2.3.20.0180, research and development project

Name: Spolupráce mezi Masarykovou univerzitou a Karolinska Institutet, Stockholm na poli biomedicíny

GA17-16680S, research and development project

Name: Nové postupy pro určení aktivity dráhy planární buněčné polarity (PCP)

Investor: Czech Science Foundation

LM2015043, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/E/0965/2016, interní kód MU

Name: Popis nově identifikovaného mechanizmu sekrece a parakrinní signalizace Wnt ligandů v průběhů embryogeneze centrální nervové soustavy savců (Acronym: Sekrece a signalizace Wnt ligandů ve vývoji CNS)

Investor: Masaryk University, Promoting quality excellence

23/2016, interní kód MU

Name: Jsou morfogeny z rodiny Wnt hormony?

Investor: Other - National

90043, large research infrastructures

Name: CIISB

90062, large research infrastructures

Name: Czech-BioImaging

Citovat

KAISER, Karol, D. GYLLBORG, J. PROCHAZKA, Alena SALAŠOVÁ, Petra KOMPANÍKOVÁ, F.L. MOLINA, R. LAGUNA-GOYA, Tomasz Witold RADASZKIEWICZ, Jakub HARNOŠ, M. PROCHAZKOVA, David POTĚŠIL, R.A. BARKER, A.G. CASADO, Zbyněk ZDRÁHAL, R. SEDLACEK, E. ARENAS, Juan Carlos VILLAESCUSA and Vítězslav BRYJA. WNT5A is transported via lipoprotein particles in the cerebrospinal fluid to regulate hindbrain morphogenesis. Nature Communications. London: Nature Publishing Group, 2019, vol. 10, No 1, p. 1-15. ISSN 2041-1723. Available from: https://dx.doi.org/10.1038/s41467-019-09298-4.

@article{1519376,
   author = {Kaiser, Karol and Gyllborg, D. and Prochazka, J. and Salašová, Alena and Kompaníková, Petra and Molina, F.L. and LagunaandGoya, R. and Radaszkiewicz, Tomasz Witold and Harnoš, Jakub and Prochazkova, M. and Potěšil, David and Barker, R.A. and Casado, A.G. and Zdráhal, Zbyněk and Sedlacek, R. and Arenas, E. and Villaescusa, Juan Carlos and Bryja, Vítězslav},
   article_location = {London},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41467-019-09298-4},
   keywords = {LOW-DENSITY LIPOPROTEINS; CELLS; PROTEINS; RECEPTOR; EXPRESSION; MUTATIONS; SECRETION; PROTEOME; HEDGEHOG; SYSTEM},
   language = {eng},
   issn = {2041-1723},
   journal = {Nature Communications},
   title = {WNT5A is transported via lipoprotein particles in the cerebrospinal fluid to regulate hindbrain morphogenesis},
   url = {https://www.nature.com/articles/s41467-019-09298-4},
   volume = {10},
   year = {2019}
}

TY  - JOUR
ID  - 1519376
AU  - Kaiser, Karol - Gyllborg, D. - Prochazka, J. - Salašová, Alena - Kompaníková, Petra - Molina, F.L. - Laguna-Goya, R. - Radaszkiewicz, Tomasz Witold - Harnoš, Jakub - Prochazkova, M. - Potěšil, David - Barker, R.A. - Casado, A.G. - Zdráhal, Zbyněk - Sedlacek, R. - Arenas, E. - Villaescusa, Juan Carlos - Bryja, Vítězslav
PY  - 2019
TI  - WNT5A is transported via lipoprotein particles in the cerebrospinal fluid to regulate hindbrain morphogenesis
JF  - Nature Communications
VL  - 10
IS  - 1
SP  - 1-15
EP  - 1-15
PB  - Nature Publishing Group
SN  - 20411723
KW  - LOW-DENSITY LIPOPROTEINS
KW  - CELLS
KW  - PROTEINS
KW  - RECEPTOR
KW  - EXPRESSION
KW  - MUTATIONS
KW  - SECRETION
KW  - PROTEOME
KW  - HEDGEHOG
KW  - SYSTEM
UR  - https://www.nature.com/articles/s41467-019-09298-4
L2  - https://www.nature.com/articles/s41467-019-09298-4
N2  - WNTs are lipid-modified proteins that control multiple functions in development and disease via short- and long-range signaling. However, it is unclear how these hydrophobic molecules spread over long distances in the mammalian brain. Here we show that WNT5A is produced by the choroid plexus (ChP) of the developing hindbrain, but not the telencephalon, in both mouse and human. Since the ChP produces and secretes the cerebrospinal fluid (CSF), we examine the presence of WNT5A in the CSF and find that it is associated with lipoprotein particles rather than exosomes. Moreover, since the CSF flows along the apical surface of hindbrain progenitors not expressing Wnt5a, we examined whether deletion of Wnt5a in the ChP controls their function and find that cerebellar morphogenesis is impaired. Our study thus identifies the CSF as a route and lipoprotein particles as a vehicle for long-range transport of biologically active WNT in the central nervous system.
ER  -

KAISER, Karol, D. GYLLBORG, J. PROCHAZKA, Alena SALAŠOVÁ, Petra KOMPANÍKOVÁ, F.L. MOLINA, R. LAGUNA-GOYA, Tomasz Witold RADASZKIEWICZ, Jakub HARNOŠ, M. PROCHAZKOVA, David POTĚŠIL, R.A. BARKER, A.G. CASADO, Zbyněk ZDRÁHAL, R. SEDLACEK, E. ARENAS, Juan Carlos VILLAESCUSA and Vítězslav BRYJA. WNT5A is transported via lipoprotein particles in the cerebrospinal fluid to regulate hindbrain morphogenesis. \textit{Nature Communications}. London: Nature Publishing Group, 2019, vol.~10, No~1, p.~1-15. ISSN~2041-1723. Available from: https://dx.doi.org/10.1038/s41467-019-09298-4.

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