J 2019

Cisplatin enhances cell stiffness and decreases invasiveness rate in prostate cancer cells by actin accumulation

RAUDENSKÁ, Martina, Monika KRATOCHVÍLOVÁ, Tomáš VIČAR, Jaromír GUMULEC, Jan BALVAN et. al.

Basic information

Original name

Cisplatin enhances cell stiffness and decreases invasiveness rate in prostate cancer cells by actin accumulation

Authors

RAUDENSKÁ, Martina (203 Czech Republic, belonging to the institution), Monika KRATOCHVÍLOVÁ (203 Czech Republic, belonging to the institution), Tomáš VIČAR (203 Czech Republic, belonging to the institution), Jaromír GUMULEC (203 Czech Republic, belonging to the institution), Jan BALVAN (203 Czech Republic, belonging to the institution), Hana POLANSKÁ (203 Czech Republic, belonging to the institution), Jan PŘIBYL (203 Czech Republic, belonging to the institution) and Michal MASAŘÍK (203 Czech Republic, guarantor, belonging to the institution)

Edition

Scientific reports, London, NATURE PUBLISHING GROUP, 2019, 2045-2322

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 3.998

RIV identification code

RIV/00216224:14110/19:00107357

Organization unit

Faculty of Medicine

DOI

UT WoS

000458017800128

Keywords in English

prostate cancer cells

Tags

Tags

International impact, Reviewed
Změněno: 10/10/2024 09:38, Ing. Monika Szurmanová, Ph.D.

Abstract

V originále

We focused on the biomechanical and morphological characteristics of prostate cancer cells and their changes resulting from the effect of docetaxel, cisplatin, and long-term zinc supplementation. Cell population surviving the treatment was characterized as follows: cell stiffness was assessed by atomic force microscopy, cell motility and invasion capacity were determined by colony forming assay, wound healing assay, coherence-controlled holographic microscopy, and real-time cell analysis. Cells of metastatic origin exhibited lower height than cells derived from the primary tumour. Cell dry mass and CAV1 gene expression followed similar trends as cell stiffness. Docetaxel-and cisplatin-surviving cells had higher stiffness, and decreased motility and invasive potential as compared to non-treated cells. This effect was not observed in zinc(II)-treated cells. We presume that cell stiffness changes may represent an important overlooked effect of cisplatin-based anti-cancer drugs. Atomic force microscopy and confocal microscopy data images used in our study are available for download in the Zenodo repository.

Links

GA18-24089S, research and development project
Name: Kvantitativní fázová mikroskopie pro 3D kvalitativní charakterizaci nádorových buněk
Investor: Czech Science Foundation
LM2015043, research and development project
Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)
Investor: Ministry of Education, Youth and Sports of the CR
MUNI/A/1553/2018, interní kód MU
Name: Genetické, environmentální a tkáňové charakteristiky vybraných patologických stavů a nemocí (Acronym: genetika; stres; biomateriály; tkáňové kultury)
Investor: Masaryk University, Category A
ROZV/24/LF/2018, interní kód MU
Name: LF - Příspěvek na IP 2108
Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects
90043, large research infrastructures
Name: CIISB