A Conditioning Sciatic Nerve Lesion Triggers a Pro-regenerative
State in Primary Sensory Neurons Also of Dorsal Root Ganglia
Non-associated With the Damaged Nerve

J 2019

A Conditioning Sciatic Nerve Lesion Triggers a Pro-regenerative State in Primary Sensory Neurons Also of Dorsal Root Ganglia Non-associated With the Damaged Nerve

DUBOVÝ, Petr, Ilona KLUSÁKOVÁ, Ivana HRADILOVÁ SVÍŽENSKÁ, Václav BRÁZDA, Marcela KOHOUTKOVÁ et. al.

Basic information

Original name

A Conditioning Sciatic Nerve Lesion Triggers a Pro-regenerative State in Primary Sensory Neurons Also of Dorsal Root Ganglia Non-associated With the Damaged Nerve

Authors

DUBOVÝ, Petr (203 Czech Republic, guarantor, belonging to the institution), Ilona KLUSÁKOVÁ (203 Czech Republic, belonging to the institution), Ivana HRADILOVÁ SVÍŽENSKÁ (203 Czech Republic, belonging to the institution), Václav BRÁZDA (203 Czech Republic, belonging to the institution), Marcela KOHOUTKOVÁ (203 Czech Republic, belonging to the institution) and Marek JOUKAL (203 Czech Republic, belonging to the institution)

Edition

Frontiers in Cellular Neuroscience, Lausanne, Frontiers, 2019, 1662-5102

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30106 Anatomy and morphology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.921

RIV identification code

RIV/00216224:14110/19:00107358

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3389/fncel.2019.00011

UT WoS

000457649800001

Keywords in English

unilateral nerve injury; primary sensory neurons; pro-regenerative state; GAP-43; SCG-10; IL-6; ulnar nerve crush; neurite outgrowth assay

Abstract

V originále

The primary sensory neurons of dorsal root ganglia (DRG) are a very useful model to study the neuronal regenerative program that is a prerequisite for successful axon regeneration after peripheral nerve injury. Seven days after a unilateral sciatic nerve injury by compression or transection, we detected a bilateral increase in growth-associated protein-43 (GAP-43) and superior cervical ganglion-10 (SCG-10) mRNA and protein levels not only in DRG neurons of lumbar spinal cord segments (L4-L5) associated with injured nerve, but also in remote cervical segments (C6-C8). The increase in regeneration-associated proteins in the cervical DRG neurons was associated with the greater length of regenerated axons 1 day after ulnar nerve crush following prior sciatic nerve injury as compared to controls with only ulnar nerve crush. The increased axonal regeneration capacity of cervical DRG neurons after a prior conditioning sciatic nerve lesion was confirmed by neurite outgrowth assay of in vitro cultivated DRG neurons. Intrathecal injection of IL-6 or a JAK2 inhibitor (AG490) revealed a role for the IL-6 signaling pathway in activating the pro-regenerative state in remote DRG neurons. Our results suggest that the pro-regenerative state induced in the DRG neurons non-associated with the injured nerve reflects a systemic reaction of these neurons to unilateral sciatic nerve injury.

Links

GA16-08508S, research and development project

Name: Zvýšení endogenního regeneračního programu a jeho aktivace zprostředkovaná cytokiny/chemokiny v neuronech ganglií bez spojení s poškozeným nervem (Acronym: ERP)

Investor: Czech Science Foundation

Citovat

DUBOVÝ, Petr, Ilona KLUSÁKOVÁ, Ivana HRADILOVÁ SVÍŽENSKÁ, Václav BRÁZDA, Marcela KOHOUTKOVÁ and Marek JOUKAL. A Conditioning Sciatic Nerve Lesion Triggers a Pro-regenerative State in Primary Sensory Neurons Also of Dorsal Root Ganglia Non-associated With the Damaged Nerve. Frontiers in Cellular Neuroscience. Lausanne: Frontiers, 2019, vol. 13, No 11, p. 1-16. ISSN 1662-5102. Available from: https://dx.doi.org/10.3389/fncel.2019.00011.

@article{1521116,
   author = {Dubový, Petr and Klusáková, Ilona and Hradilová Svíženská, Ivana and Brázda, Václav and Kohoutková, Marcela and Joukal, Marek},
   article_location = {Lausanne},
   article_number = {11},
   doi = {http://dx.doi.org/10.3389/fncel.2019.00011},
   keywords = {unilateral nerve injury; primary sensory neurons; pro-regenerative state; GAP-43; SCG-10; IL-6; ulnar nerve crush; neurite outgrowth assay},
   language = {eng},
   issn = {1662-5102},
   journal = {Frontiers in Cellular Neuroscience},
   title = {A Conditioning Sciatic Nerve Lesion Triggers a Pro-regenerative State in Primary Sensory Neurons Also of Dorsal Root Ganglia Non-associated With the Damaged Nerve},
   url = {http://dx.doi.org/10.3389/fncel.2019.00011},
   volume = {13},
   year = {2019}
}

TY  - JOUR
ID  - 1521116
AU  - Dubový, Petr - Klusáková, Ilona - Hradilová Svíženská, Ivana - Brázda, Václav - Kohoutková, Marcela - Joukal, Marek
PY  - 2019
TI  - A Conditioning Sciatic Nerve Lesion Triggers a Pro-regenerative State in Primary Sensory Neurons Also of Dorsal Root Ganglia Non-associated With the Damaged Nerve
JF  - Frontiers in Cellular Neuroscience
VL  - 13
IS  - 11
SP  - 1-16
EP  - 1-16
PB  - Frontiers
SN  - 16625102
KW  - unilateral nerve injury
KW  - primary sensory neurons
KW  - pro-regenerative state
KW  - GAP-43
KW  - SCG-10
KW  - IL-6
KW  - ulnar nerve crush
KW  - neurite outgrowth assay
UR  - http://dx.doi.org/10.3389/fncel.2019.00011
L2  - http://dx.doi.org/10.3389/fncel.2019.00011
N2  - The primary sensory neurons of dorsal root ganglia (DRG) are a very useful model to study the neuronal regenerative program that is a prerequisite for successful axon regeneration after peripheral nerve injury. Seven days after a unilateral sciatic nerve injury by compression or transection, we detected a bilateral increase in growth-associated protein-43 (GAP-43) and superior cervical ganglion-10 (SCG-10) mRNA and protein levels not only in DRG neurons of lumbar spinal cord segments (L4-L5) associated with injured nerve, but also in remote cervical segments (C6-C8). The increase in regeneration-associated proteins in the cervical DRG neurons was associated with the greater length of regenerated axons 1 day after ulnar nerve crush following prior sciatic nerve injury as compared to controls with only ulnar nerve crush. The increased axonal regeneration capacity of cervical DRG neurons after a prior conditioning sciatic nerve lesion was confirmed by neurite outgrowth assay of in vitro cultivated DRG neurons. Intrathecal injection of IL-6 or a JAK2 inhibitor (AG490) revealed a role for the IL-6 signaling pathway in activating the pro-regenerative state in remote DRG neurons. Our results suggest that the pro-regenerative state induced in the DRG neurons non-associated with the injured nerve reflects a systemic reaction of these neurons to unilateral sciatic nerve injury.
ER  -

DUBOVÝ, Petr, Ilona KLUSÁKOVÁ, Ivana HRADILOVÁ SVÍŽENSKÁ, Václav BRÁZDA, Marcela KOHOUTKOVÁ and Marek JOUKAL. A Conditioning Sciatic Nerve Lesion Triggers a Pro-regenerative State in Primary Sensory Neurons Also of Dorsal Root Ganglia Non-associated With the Damaged Nerve. \textit{Frontiers in Cellular Neuroscience}. Lausanne: Frontiers, 2019, vol.~13, No~11, p.~1-16. ISSN~1662-5102. Available from: https://dx.doi.org/10.3389/fncel.2019.00011.

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