| DUBOVÝ, Petr, Ilona KLUSÁKOVÁ, Ivana HRADILOVÁ SVÍŽENSKÁ, Václav BRÁZDA, Marcela KOHOUTKOVÁ and Marek JOUKAL. A Conditioning Sciatic Nerve Lesion Triggers a Pro-regenerative State in Primary Sensory Neurons Also of Dorsal Root Ganglia Non-associated With the Damaged Nerve. Frontiers in Cellular Neuroscience. Lausanne: Frontiers, 2019, vol. 13, No 11, p. 1-16. ISSN 1662-5102. Available from: https://dx.doi.org/10.3389/fncel.2019.00011. |
Other formats:
BibTeX
LaTeX
RIS
@article{1521116,
author = {Dubový, Petr and Klusáková, Ilona and Hradilová Svíženská, Ivana and Brázda, Václav and Kohoutková, Marcela and Joukal, Marek},
article_location = {Lausanne},
article_number = {11},
doi = {http://dx.doi.org/10.3389/fncel.2019.00011},
keywords = {unilateral nerve injury; primary sensory neurons; pro-regenerative state; GAP-43; SCG-10; IL-6; ulnar nerve crush; neurite outgrowth assay},
language = {eng},
issn = {1662-5102},
journal = {Frontiers in Cellular Neuroscience},
title = {A Conditioning Sciatic Nerve Lesion Triggers a Pro-regenerative State in Primary Sensory Neurons Also of Dorsal Root Ganglia Non-associated With the Damaged Nerve},
url = {http://dx.doi.org/10.3389/fncel.2019.00011},
volume = {13},
year = {2019}
}
TY - JOUR
ID - 1521116
AU - Dubový, Petr - Klusáková, Ilona - Hradilová Svíženská, Ivana - Brázda, Václav - Kohoutková, Marcela - Joukal, Marek
PY - 2019
TI - A Conditioning Sciatic Nerve Lesion Triggers a Pro-regenerative State in Primary Sensory Neurons Also of Dorsal Root Ganglia Non-associated With the Damaged Nerve
JF - Frontiers in Cellular Neuroscience
VL - 13
IS - 11
SP - 1-16
EP - 1-16
PB - Frontiers
SN - 16625102
KW - unilateral nerve injury
KW - primary sensory neurons
KW - pro-regenerative state
KW - GAP-43
KW - SCG-10
KW - IL-6
KW - ulnar nerve crush
KW - neurite outgrowth assay
UR - http://dx.doi.org/10.3389/fncel.2019.00011
L2 - http://dx.doi.org/10.3389/fncel.2019.00011
N2 - The primary sensory neurons of dorsal root ganglia (DRG) are a very useful model to study the neuronal regenerative program that is a prerequisite for successful axon regeneration after peripheral nerve injury. Seven days after a unilateral sciatic nerve injury by compression or transection, we detected a bilateral increase in growth-associated protein-43 (GAP-43) and superior cervical ganglion-10 (SCG-10) mRNA and protein levels not only in DRG neurons of lumbar spinal cord segments (L4-L5) associated with injured nerve, but also in remote cervical segments (C6-C8). The increase in regeneration-associated proteins in the cervical DRG neurons was associated with the greater length of regenerated axons 1 day after ulnar nerve crush following prior sciatic nerve injury as compared to controls with only ulnar nerve crush. The increased axonal regeneration capacity of cervical DRG neurons after a prior conditioning sciatic nerve lesion was confirmed by neurite outgrowth assay of in vitro cultivated DRG neurons. Intrathecal injection of IL-6 or a JAK2 inhibitor (AG490) revealed a role for the IL-6 signaling pathway in activating the pro-regenerative state in remote DRG neurons. Our results suggest that the pro-regenerative state induced in the DRG neurons non-associated with the injured nerve reflects a systemic reaction of these neurons to unilateral sciatic nerve injury.
ER -
DUBOVÝ, Petr, Ilona KLUSÁKOVÁ, Ivana HRADILOVÁ SVÍŽENSKÁ, Václav BRÁZDA, Marcela KOHOUTKOVÁ and Marek JOUKAL. A Conditioning Sciatic Nerve Lesion Triggers a Pro-regenerative State in Primary Sensory Neurons Also of Dorsal Root Ganglia Non-associated With the Damaged Nerve. \textit{Frontiers in Cellular Neuroscience}. Lausanne: Frontiers, 2019, vol.~13, No~11, p.~1-16. ISSN~1662-5102. Available from: https://dx.doi.org/10.3389/fncel.2019.00011.
|
