Lytic and genomic properties of spontaneous host-range Kayvirus
mutants prove their suitability for upgrading phage
therapeutics against staphylococci

J 2019

Lytic and genomic properties of spontaneous host-range Kayvirus mutants prove their suitability for upgrading phage therapeutics against staphylococci

BOTKA, Tibor, Roman PANTŮČEK, Ivana MAŠLAŇOVÁ, Martin BENEŠÍK, Petr PETRÁŠ et. al.

Basic information

Original name

Lytic and genomic properties of spontaneous host-range Kayvirus mutants prove their suitability for upgrading phage therapeutics against staphylococci

Authors

BOTKA, Tibor (203 Czech Republic, belonging to the institution), Roman PANTŮČEK (203 Czech Republic, guarantor, belonging to the institution), Ivana MAŠLAŇOVÁ (203 Czech Republic, belonging to the institution), Martin BENEŠÍK (203 Czech Republic, belonging to the institution), Petr PETRÁŠ (203 Czech Republic), Vladislava RŮŽIČKOVÁ (203 Czech Republic, belonging to the institution), Pavla HAVLÍČKOVÁ (203 Czech Republic, belonging to the institution), Marian VARGA (203 Czech Republic, belonging to the institution), Helena ŽEMLIČKOVÁ (203 Czech Republic), Ivana KOLÁČKOVÁ (203 Czech Republic), Martina FLORIANOVÁ (203 Czech Republic), Vladislav JAKUBŮ (203 Czech Republic), Renata KARPÍŠKOVÁ (203 Czech Republic) and Jiří DOŠKAŘ (203 Czech Republic, belonging to the institution)

Edition

Scientific Reports, London, Nature Publishing Group, 2019, 2045-2322

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10603 Genetics and heredity

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.998

RIV identification code

RIV/00216224:14310/19:00107363

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1038/s41598-019-41868-w

UT WoS

000462990000017

Keywords in English

Kayvirus; Viral genetics; Phage Therapy; Methicillin-Resistant Staphylococcus aureus; Host Range; Genetic Polymorphisms

Abstract

V originále

Lytic bacteriophages are valuable therapeutic agents against bacterial infections. There is continual effort to obtain new phages to increase the effectivity of phage preparations against emerging phage-resistant strains. Here we described the genomic diversity of spontaneous host-range mutants of kayvirus 812. Five mutant phages were isolated as rare plaques on phage-resistant Staphylococcus aureus strains. The host range of phage 812-derived mutants was 42% higher than the wild type, determined on a set of 186 methicillin-resistant S. aureus strains representing the globally circulating human and livestock-associated clones. Comparative genomics revealed that single-nucleotide polymorphisms from the parental phage 812 population were fixed in next-step mutants, mostly in genes for tail and baseplate components, and the acquired point mutations led to diverse receptor binding proteins in the phage mutants. Numerous genome changes associated with rearrangements between direct repeat motifs or intron loss were found. Alterations occurred in host-takeover and terminal genomic regions or the endolysin gene of mutants that exhibited the highest lytic activity, which implied various mechanisms of overcoming bacterial resistance. The genomic data revealed that Kayvirus spontaneous mutants are free from undesirable genes and their lytic properties proved their suitability for rapidly updating phage therapeutics.

Links

GA18-13064S, research and development project

Name: Analýza interakcí mezi polyvalentním terapeutickým fágem druhu Twort-like a jeho hostitelem Staphylococcus aureus

Investor: Czech Science Foundation

NV16-29916A, research and development project

Name: Využití bakteriofágů v léčbě nozokomiálních infekcí spojených s multirezistencí či tvorbou biofilmu

QJ1510216, research and development project

Name: Fágová terapie infekcí vyvolaných Staphylococcus aureus v chovech hospodářských zvířat (Acronym: StafyFag)

Investor: Ministry of Agriculture of the CR

Files attached

s41598-019-41868-w.pdf

Request the author's version of the file

Citovat

BOTKA, Tibor, Roman PANTŮČEK, Ivana MAŠLAŇOVÁ, Martin BENEŠÍK, Petr PETRÁŠ, Vladislava RŮŽIČKOVÁ, Pavla HAVLÍČKOVÁ, Marian VARGA, Helena ŽEMLIČKOVÁ, Ivana KOLÁČKOVÁ, Martina FLORIANOVÁ, Vladislav JAKUBŮ, Renata KARPÍŠKOVÁ and Jiří DOŠKAŘ. Lytic and genomic properties of spontaneous host-range Kayvirus mutants prove their suitability for upgrading phage therapeutics against staphylococci. Scientific Reports. London: Nature Publishing Group, 2019, vol. 9, No 1, p. 5475-5486. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-019-41868-w.

@article{1522076,
   author = {Botka, Tibor and Pantůček, Roman and Mašlaňová, Ivana and Benešík, Martin and Petráš, Petr and Růžičková, Vladislava and Havlíčková, Pavla and Varga, Marian and Žemličková, Helena and Koláčková, Ivana and Florianová, Martina and Jakubů, Vladislav and Karpíšková, Renata and Doškař, Jiří},
   article_location = {London},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-019-41868-w},
   keywords = {Kayvirus; Viral genetics; Phage Therapy; Methicillin-Resistant Staphylococcus aureus; Host Range; Genetic Polymorphisms},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific Reports},
   title = {Lytic and genomic properties of spontaneous host-range Kayvirus mutants prove their suitability for upgrading phage therapeutics against staphylococci},
   url = {https://www.nature.com/articles/s41598-019-41868-w},
   volume = {9},
   year = {2019}
}

TY  - JOUR
ID  - 1522076
AU  - Botka, Tibor - Pantůček, Roman - Mašlaňová, Ivana - Benešík, Martin - Petráš, Petr - Růžičková, Vladislava - Havlíčková, Pavla - Varga, Marian - Žemličková, Helena - Koláčková, Ivana - Florianová, Martina - Jakubů, Vladislav - Karpíšková, Renata - Doškař, Jiří
PY  - 2019
TI  - Lytic and genomic properties of spontaneous host-range Kayvirus mutants prove their suitability for upgrading phage therapeutics against staphylococci
JF  - Scientific Reports
VL  - 9
IS  - 1
SP  - 5475
EP  - 5475
PB  - Nature Publishing Group
SN  - 20452322
KW  - Kayvirus
KW  - Viral genetics
KW  - Phage Therapy
KW  - Methicillin-Resistant Staphylococcus aureus
KW  - Host Range
KW  - Genetic Polymorphisms
UR  - https://www.nature.com/articles/s41598-019-41868-w
L2  - https://www.nature.com/articles/s41598-019-41868-w
N2  - Lytic bacteriophages are valuable therapeutic agents against bacterial infections. There is continual effort to obtain new phages to increase the effectivity of phage preparations against emerging phage-resistant strains. Here we described the genomic diversity of spontaneous host-range mutants of kayvirus 812. Five mutant phages were isolated as rare plaques on phage-resistant Staphylococcus aureus strains. The host range of phage 812-derived mutants was 42% higher than the wild type, determined on a set of 186 methicillin-resistant S. aureus strains representing the globally circulating human and livestock-associated clones. Comparative genomics revealed that single-nucleotide polymorphisms from the parental phage 812 population were fixed in next-step mutants, mostly in genes for tail and baseplate components, and the acquired point mutations led to diverse receptor binding proteins in the phage mutants. Numerous genome changes associated with rearrangements between direct repeat motifs or intron loss were found. Alterations occurred in host-takeover and terminal genomic regions or the endolysin gene of mutants that exhibited the highest lytic activity, which implied various mechanisms of overcoming bacterial resistance. The genomic data revealed that Kayvirus spontaneous mutants are free from undesirable genes and their lytic properties proved their suitability for rapidly updating phage therapeutics.
ER  -

BOTKA, Tibor, Roman PANTŮČEK, Ivana MAŠLAŇOVÁ, Martin BENEŠÍK, Petr PETRÁŠ, Vladislava RŮŽIČKOVÁ, Pavla HAVLÍČKOVÁ, Marian VARGA, Helena ŽEMLIČKOVÁ, Ivana KOLÁČKOVÁ, Martina FLORIANOVÁ, Vladislav JAKUBŮ, Renata KARPÍŠKOVÁ and Jiří DOŠKAŘ. Lytic and genomic properties of spontaneous host-range Kayvirus mutants prove their suitability for upgrading phage therapeutics against staphylococci. \textit{Scientific Reports}. London: Nature Publishing Group, 2019, vol.~9, No~1, p.~5475-5486. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-019-41868-w.

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