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@article{1522296,
author = {Lipková, Jolana and Šplíchal, Zbyněk and Jurajda, Michal and Madarászová, Tereza and Vašků, Anna and Smrčka, Martin and Ďuriš, Kamil},
article_location = {Praha},
article_number = {1},
doi = {http://dx.doi.org/10.14735/amcsnn201953},
keywords = {subarachnoid haemorrhage; early brain injury; inflammation; apoptosis; microRNA; perforation model},
language = {eng},
issn = {1210-7859},
journal = {Česká a slovenská neurologie a neurochirurgie},
title = {TNF alpha and microRNA-15b expression changes in experimental model of subarachnoid haemorrhage},
url = {http://dx.doi.org/10.14735/amcsnn201953},
volume = {82},
year = {2019}
}
TY - JOUR
ID - 1522296
AU - Lipková, Jolana - Šplíchal, Zbyněk - Jurajda, Michal - Madarászová, Tereza - Vašků, Anna - Smrčka, Martin - Ďuriš, Kamil
PY - 2019
TI - TNF alpha and microRNA-15b expression changes in experimental model of subarachnoid haemorrhage
JF - Česká a slovenská neurologie a neurochirurgie
VL - 82
IS - 1
SP - 53-59
EP - 53-59
PB - Česká lékařská společnost J.E. Purkyně
SN - 12107859
KW - subarachnoid haemorrhage
KW - early brain injury
KW - inflammation
KW - apoptosis
KW - microRNA
KW - perforation model
UR - http://dx.doi.org/10.14735/amcsnn201953
L2 - http://dx.doi.org/10.14735/amcsnn201953
N2 - Aim: The aim of the study was to investigate expression changes of pro-inflammatory and proapoptotic cytokine tumor necrosis factor alpha (TNF alpha) and microRNAs (miRNAs) involved in its regulation in early pathophysiological changes after subarachnoid haemorrhage (SAH). Materials and methods: MiRNAs (miR-125b, miR-146a, miR-346, miR-155, miR-15b) and mRNA (TNF alpha) expression were determined by quantitative real-time polymerase chain reaction in brain tissue samples. A total of 88 animals were divided to Sham (control surgery without induction of SAH), Mild SAH, Severe SAH groups in following time-points: 2, 4, 6 and 8 h (n = 7 per group); including 4 animals used as an absolute control. Results: We have found a statistically significant difference in TNF alpha expression between Sham and Severe SAH groups at all the time-points (p < 0.05), between Sham and Mild SAH groups 4 h after induction of SAH (p < 0.05) and between Mild and Severe SAH groups at 2 and 6 h time-points (p < 0.05). Furthermore, a significant difference in miR-15b expression between Sham and Severe SAH groups was observed 8 h after SAH (p < 0.05). All the other microRNAs have not been significantly changed. Conclusions: SAH was associated with an early increase in TNF alpha and miR-15b expression especially in Severe SAH group. Despite complex cross-regulation between cytokines and miRNA, any information about the activation of inflammation/apoptotic mechanisms within a few hours after SAH may improve our knowledge of SAH pathophysiology. Furthermore, it can lead to therapeutic improvement using a combination of both pro-apoptotic markers TNF alpha and miR-15b.
ER -
LIPKOVÁ, Jolana, Zbyněk ŠPLÍCHAL, Michal JURAJDA, Tereza MADARÁSZOVÁ, Anna VAŠKŮ, Martin SMRČKA and Kamil ĎURIŠ. TNF alpha and microRNA-15b expression changes in experimental model of subarachnoid haemorrhage. \textit{Česká a slovenská neurologie a neurochirurgie}. Praha: Česká lékařská společnost J.E. Purkyně, 2019, vol.~82, No~1, p.~53-59. ISSN~1210-7859. Available from: https://dx.doi.org/10.14735/amcsnn201953.
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