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@article{1522296, author = {Lipková, Jolana and Šplíchal, Zbyněk and Jurajda, Michal and Madarászová, Tereza and Vašků, Anna and Smrčka, Martin and Ďuriš, Kamil}, article_location = {Praha}, article_number = {1}, doi = {http://dx.doi.org/10.14735/amcsnn201953}, keywords = {subarachnoid haemorrhage; early brain injury; inflammation; apoptosis; microRNA; perforation model}, language = {eng}, issn = {1210-7859}, journal = {Česká a slovenská neurologie a neurochirurgie}, title = {TNF alpha and microRNA-15b expression changes in experimental model of subarachnoid haemorrhage}, url = {http://dx.doi.org/10.14735/amcsnn201953}, volume = {82}, year = {2019} }
TY - JOUR ID - 1522296 AU - Lipková, Jolana - Šplíchal, Zbyněk - Jurajda, Michal - Madarászová, Tereza - Vašků, Anna - Smrčka, Martin - Ďuriš, Kamil PY - 2019 TI - TNF alpha and microRNA-15b expression changes in experimental model of subarachnoid haemorrhage JF - Česká a slovenská neurologie a neurochirurgie VL - 82 IS - 1 SP - 53-59 EP - 53-59 PB - Česká lékařská společnost J.E. Purkyně SN - 12107859 KW - subarachnoid haemorrhage KW - early brain injury KW - inflammation KW - apoptosis KW - microRNA KW - perforation model UR - http://dx.doi.org/10.14735/amcsnn201953 L2 - http://dx.doi.org/10.14735/amcsnn201953 N2 - Aim: The aim of the study was to investigate expression changes of pro-inflammatory and proapoptotic cytokine tumor necrosis factor alpha (TNF alpha) and microRNAs (miRNAs) involved in its regulation in early pathophysiological changes after subarachnoid haemorrhage (SAH). Materials and methods: MiRNAs (miR-125b, miR-146a, miR-346, miR-155, miR-15b) and mRNA (TNF alpha) expression were determined by quantitative real-time polymerase chain reaction in brain tissue samples. A total of 88 animals were divided to Sham (control surgery without induction of SAH), Mild SAH, Severe SAH groups in following time-points: 2, 4, 6 and 8 h (n = 7 per group); including 4 animals used as an absolute control. Results: We have found a statistically significant difference in TNF alpha expression between Sham and Severe SAH groups at all the time-points (p < 0.05), between Sham and Mild SAH groups 4 h after induction of SAH (p < 0.05) and between Mild and Severe SAH groups at 2 and 6 h time-points (p < 0.05). Furthermore, a significant difference in miR-15b expression between Sham and Severe SAH groups was observed 8 h after SAH (p < 0.05). All the other microRNAs have not been significantly changed. Conclusions: SAH was associated with an early increase in TNF alpha and miR-15b expression especially in Severe SAH group. Despite complex cross-regulation between cytokines and miRNA, any information about the activation of inflammation/apoptotic mechanisms within a few hours after SAH may improve our knowledge of SAH pathophysiology. Furthermore, it can lead to therapeutic improvement using a combination of both pro-apoptotic markers TNF alpha and miR-15b. ER -
LIPKOVÁ, Jolana, Zbyněk ŠPLÍCHAL, Michal JURAJDA, Tereza MADARÁSZOVÁ, Anna VAŠKŮ, Martin SMRČKA and Kamil ĎURIŠ. TNF alpha and microRNA-15b expression changes in experimental model of subarachnoid haemorrhage. \textit{Česká a slovenská neurologie a neurochirurgie}. Praha: Česká lékařská společnost J.E. Purkyně, 2019, vol.~82, No~1, p.~53-59. ISSN~1210-7859. Available from: https://dx.doi.org/10.14735/amcsnn201953.
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