Detailed Information on Publication Record
2019
TNF alpha and microRNA-15b expression changes in experimental model of subarachnoid haemorrhage
LIPKOVÁ, Jolana, Zbyněk ŠPLÍCHAL, Michal JURAJDA, Tereza MADARÁSZOVÁ, Anna VAŠKŮ et. al.Basic information
Original name
TNF alpha and microRNA-15b expression changes in experimental model of subarachnoid haemorrhage
Authors
LIPKOVÁ, Jolana (203 Czech Republic, belonging to the institution), Zbyněk ŠPLÍCHAL (203 Czech Republic, belonging to the institution), Michal JURAJDA (203 Czech Republic, guarantor, belonging to the institution), Tereza MADARÁSZOVÁ (203 Czech Republic, belonging to the institution), Anna VAŠKŮ (203 Czech Republic, belonging to the institution), Martin SMRČKA (203 Czech Republic, belonging to the institution) and Kamil ĎURIŠ (203 Czech Republic, belonging to the institution)
Edition
Česká a slovenská neurologie a neurochirurgie, Praha, Česká lékařská společnost J.E. Purkyně, 2019, 1210-7859
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30103 Neurosciences
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 0.377
RIV identification code
RIV/00216224:14110/19:00107364
Organization unit
Faculty of Medicine
UT WoS
000458015800007
Keywords in English
subarachnoid haemorrhage; early brain injury; inflammation; apoptosis; microRNA; perforation model
Tags
International impact, Reviewed
Změněno: 17/4/2019 14:22, Soňa Böhmová
Abstract
V originále
Aim: The aim of the study was to investigate expression changes of pro-inflammatory and proapoptotic cytokine tumor necrosis factor alpha (TNF alpha) and microRNAs (miRNAs) involved in its regulation in early pathophysiological changes after subarachnoid haemorrhage (SAH). Materials and methods: MiRNAs (miR-125b, miR-146a, miR-346, miR-155, miR-15b) and mRNA (TNF alpha) expression were determined by quantitative real-time polymerase chain reaction in brain tissue samples. A total of 88 animals were divided to Sham (control surgery without induction of SAH), Mild SAH, Severe SAH groups in following time-points: 2, 4, 6 and 8 h (n = 7 per group); including 4 animals used as an absolute control. Results: We have found a statistically significant difference in TNF alpha expression between Sham and Severe SAH groups at all the time-points (p < 0.05), between Sham and Mild SAH groups 4 h after induction of SAH (p < 0.05) and between Mild and Severe SAH groups at 2 and 6 h time-points (p < 0.05). Furthermore, a significant difference in miR-15b expression between Sham and Severe SAH groups was observed 8 h after SAH (p < 0.05). All the other microRNAs have not been significantly changed. Conclusions: SAH was associated with an early increase in TNF alpha and miR-15b expression especially in Severe SAH group. Despite complex cross-regulation between cytokines and miRNA, any information about the activation of inflammation/apoptotic mechanisms within a few hours after SAH may improve our knowledge of SAH pathophysiology. Furthermore, it can lead to therapeutic improvement using a combination of both pro-apoptotic markers TNF alpha and miR-15b.
Links
| GP14-23773P, research and development project |
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