BALANCE BETWEEN EPITHELIAL AND STROMAL MARKER EXPRESSION AND
DISTRIBUTION IN PRIMARY CULTURE MODEL OF PORCINE ENDOMETRIUM
DURING REAL-TIME CELL PROLIFERATION

J 2018

BALANCE BETWEEN EPITHELIAL AND STROMAL MARKER EXPRESSION AND DISTRIBUTION IN PRIMARY CULTURE MODEL OF PORCINE ENDOMETRIUM DURING REAL-TIME CELL PROLIFERATION

WOJTANOWICZ-MARKICZ, K., M.J. NAWROCKI, M. BRAZERT, K. OZEGOWSKA, M. JANKOWSKI et. al.

Basic information

Original name

BALANCE BETWEEN EPITHELIAL AND STROMAL MARKER EXPRESSION AND DISTRIBUTION IN PRIMARY CULTURE MODEL OF PORCINE ENDOMETRIUM DURING REAL-TIME CELL PROLIFERATION

Authors

WOJTANOWICZ-MARKICZ, K. (616 Poland), M.J. NAWROCKI (616 Poland), M. BRAZERT (616 Poland), K. OZEGOWSKA (616 Poland), M. JANKOWSKI (616 Poland), M. ROJEWSKA (616 Poland), I. KOCHEROVA (616 Poland), M. KULUS (616 Poland), Michal JEŠETA (203 Czech Republic, belonging to the institution), A. BRYJA (616 Poland), L. POROWSKI (616 Poland), D. BUKOWSKA (616 Poland), M.T. SKOWRONSKI, A. BREBOROWICZ (616 Poland), M. BRUSKA (616 Poland), M. ZABEL (616 Poland), M. NOWICKI (616 Poland), B. KEMPLSTY (616 Poland, guarantor) and P. ANTOSIK (616 Poland)

Edition

Journal of biological regulators and homeostatic agents, Silva Marina, Biolife SAS, 2018, 0393-974X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30202 Endocrinology and metabolism

Country of publisher

Italy

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.558

RIV identification code

RIV/00216224:14110/18:00106865

Organization unit

Faculty of Medicine

UT WoS

000449211700003

Keywords in English

pig; uterus cells; cytokeratin; vimentin; real-time proliferation

Abstract

V originále

The similarity between humans and pigs, when it comes to tissue morphology, makes Sus scrofa not only a good research model, but also a potential source of cells for tissue engineering. Cell samples obtained from the pig donor, could be influenced in vitro, in order to become a source of tissue material for xenotransplantation, reconstructive and regenerative medicine. Significant amounts of data point to especially major similarities in pig and human reproductive systems. Because of that, particular scientific focus is centered on research concerning porcine COCs, theca and granulosa cells in primary cultures. One of the aspects of the reproductive process, that is still largely undiscovered, is the interaction between preimplantation blastocyst and maternal uterine tissues. In this study, we used molecular analysis techniques, such as RT-qPCR and immunocytochemistry, to analyze the expression and distribution of cytokeratin 18 and panCytokeratins 8, 18 and 19 and vimentin in porcine luminal endometrial epithelial cells, coupled with analysis of their behavior in RTCA. The results have confirmed the presence of epithelial, as well as stromal cell markers in the cells, varying in levels at different stages of culture. They have also given insight into the modes of proliferation and differentiation of studied cells in in vitro culture, as well as providing additional proof for the possible mesenchymal transdifferentiation of epithelial cells.

Citovat

WOJTANOWICZ-MARKICZ, K., M.J. NAWROCKI, M. BRAZERT, K. OZEGOWSKA, M. JANKOWSKI, M. ROJEWSKA, I. KOCHEROVA, M. KULUS, Michal JEŠETA, A. BRYJA, L. POROWSKI, D. BUKOWSKA, M.T. SKOWRONSKI, A. BREBOROWICZ, M. BRUSKA, M. ZABEL, M. NOWICKI, B. KEMPLSTY and P. ANTOSIK. BALANCE BETWEEN EPITHELIAL AND STROMAL MARKER EXPRESSION AND DISTRIBUTION IN PRIMARY CULTURE MODEL OF PORCINE ENDOMETRIUM DURING REAL-TIME CELL PROLIFERATION. Journal of biological regulators and homeostatic agents. Silva Marina: Biolife SAS, 2018, vol. 32, No 5, p. 1067-1077. ISSN 0393-974X.

@article{1526417,
   author = {WojtanowiczandMarkicz, K. and Nawrocki, M.J. and Brazert, M. and Ozegowska, K. and Jankowski, M. and Rojewska, M. and Kocherova, I. and Kulus, M. and Ješeta, Michal and Bryja, A. and Porowski, L. and Bukowska, D. and Skowronski, M.T. and Breborowicz, A. and Bruska, M. and Zabel, M. and Nowicki, M. and Kemplsty, B. and Antosik, P.},
   article_location = {Silva Marina},
   article_number = {5},
   keywords = {pig; uterus cells; cytokeratin; vimentin; real-time proliferation},
   language = {eng},
   issn = {0393-974X},
   journal = {Journal of biological regulators and homeostatic agents},
   title = {BALANCE BETWEEN EPITHELIAL AND STROMAL MARKER EXPRESSION AND DISTRIBUTION IN PRIMARY CULTURE MODEL OF PORCINE ENDOMETRIUM DURING REAL-TIME CELL PROLIFERATION},
   volume = {32},
   year = {2018}
}

TY  - JOUR
ID  - 1526417
AU  - Wojtanowicz-Markicz, K. - Nawrocki, M.J. - Brazert, M. - Ozegowska, K. - Jankowski, M. - Rojewska, M. - Kocherova, I. - Kulus, M. - Ješeta, Michal - Bryja, A. - Porowski, L. - Bukowska, D. - Skowronski, M.T. - Breborowicz, A. - Bruska, M. - Zabel, M. - Nowicki, M. - Kemplsty, B. - Antosik, P.
PY  - 2018
TI  - BALANCE BETWEEN EPITHELIAL AND STROMAL MARKER EXPRESSION AND DISTRIBUTION IN PRIMARY CULTURE MODEL OF PORCINE ENDOMETRIUM DURING REAL-TIME CELL PROLIFERATION
JF  - Journal of biological regulators and homeostatic agents
VL  - 32
IS  - 5
SP  - 1067-1077
EP  - 1067-1077
PB  - Biolife SAS
SN  - 0393974X
KW  - pig
KW  - uterus cells
KW  - cytokeratin
KW  - vimentin
KW  - real-time proliferation
N2  - The similarity between humans and pigs, when it comes to tissue morphology, makes Sus scrofa not only a good research model, but also a potential source of cells for tissue engineering. Cell samples obtained from the pig donor, could be influenced in vitro, in order to become a source of tissue material for xenotransplantation, reconstructive and regenerative medicine. Significant amounts of data point to especially major similarities in pig and human reproductive systems. Because of that, particular scientific focus is centered on research concerning porcine COCs, theca and granulosa cells in primary cultures. One of the aspects of the reproductive process, that is still largely undiscovered, is the interaction between preimplantation blastocyst and maternal uterine tissues. In this study, we used molecular analysis techniques, such as RT-qPCR and immunocytochemistry, to analyze the expression and distribution of cytokeratin 18 and panCytokeratins 8, 18 and 19 and vimentin in porcine luminal endometrial epithelial cells, coupled with analysis of their behavior in RTCA. The results have confirmed the presence of epithelial, as well as stromal cell markers in the cells, varying in levels at different stages of culture. They have also given insight into the modes of proliferation and differentiation of studied cells in in vitro culture, as well as providing additional proof for the possible mesenchymal transdifferentiation of epithelial cells.
ER  -

WOJTANOWICZ-MARKICZ, K., M.J. NAWROCKI, M. BRAZERT, K. OZEGOWSKA, M. JANKOWSKI, M. ROJEWSKA, I. KOCHEROVA, M. KULUS, Michal JEŠETA, A. BRYJA, L. POROWSKI, D. BUKOWSKA, M.T. SKOWRONSKI, A. BREBOROWICZ, M. BRUSKA, M. ZABEL, M. NOWICKI, B. KEMPLSTY and P. ANTOSIK. BALANCE BETWEEN EPITHELIAL AND STROMAL MARKER EXPRESSION AND DISTRIBUTION IN PRIMARY CULTURE MODEL OF PORCINE ENDOMETRIUM DURING REAL-TIME CELL PROLIFERATION. \textit{Journal of biological regulators and homeostatic agents}. Silva Marina: Biolife SAS, 2018, vol.~32, No~5, p.~1067-1077. ISSN~0393-974X.

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