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@article{1528796,
author = {Buchta, David and Füzik, Tibor and Hrebík, Dominik and Levdansky, Yevgen and Sukeník, Lukáš and Mukhamedova, Liya and Moravcová, Jana and Vácha, Robert and Plevka, Pavel},
article_location = {London},
article_number = {MAR},
doi = {http://dx.doi.org/10.1038/s41467-019-09132-x},
keywords = {MOLECULAR-DYNAMICS; CONFORMATIONAL-CHANGES; FORCE-FIELD; CELL ENTRY; POLIOVIRUS; VIRUS; RNA; MODEL; TRANSITIONS; RECOGNITION},
language = {eng},
issn = {2041-1723},
journal = {Nature Communications},
title = {Enterovirus particles expel capsid pentamers to enable genome release},
url = {https://www.nature.com/articles/s41467-019-09132-x.pdf},
volume = {10},
year = {2019}
}
TY - JOUR
ID - 1528796
AU - Buchta, David - Füzik, Tibor - Hrebík, Dominik - Levdansky, Yevgen - Sukeník, Lukáš - Mukhamedova, Liya - Moravcová, Jana - Vácha, Robert - Plevka, Pavel
PY - 2019
TI - Enterovirus particles expel capsid pentamers to enable genome release
JF - Nature Communications
VL - 10
IS - MAR
SP - 1-9
EP - 1-9
PB - Nature Publishing Group
SN - 20411723
KW - MOLECULAR-DYNAMICS
KW - CONFORMATIONAL-CHANGES
KW - FORCE-FIELD
KW - CELL ENTRY
KW - POLIOVIRUS
KW - VIRUS
KW - RNA
KW - MODEL
KW - TRANSITIONS
KW - RECOGNITION
UR - https://www.nature.com/articles/s41467-019-09132-x.pdf
L2 - https://www.nature.com/articles/s41467-019-09132-x.pdf
N2 - Viruses from the genus Enterovirus are important human pathogens. Receptor binding or exposure to acidic pH in endosomes converts enterovirus particles to an activated state that is required for genome release. However, the mechanism of enterovirus uncoating is not well understood. Here, we use cryo-electron microscopy to visualize virions of human echovirus 18 in the process of genome release. We discover that the exit of the RNA from the particle of echovirus 18 results in a loss of one, two, or three adjacent capsid-protein pentamers. The opening in the capsid, which is more than 120 A in diameter, enables the release of the genome without the need to unwind its putative double-stranded RNA segments. We also detect capsids lacking pentamers during genome release from echovirus 30. Thus, our findings uncover a mechanism of enterovirus genome release that could become target for antiviral drugs.
ER -
BUCHTA, David, Tibor FÜZIK, Dominik HREBÍK, Yevgen LEVDANSKY, Lukáš SUKENÍK, Liya MUKHAMEDOVA, Jana MORAVCOVÁ, Robert VÁCHA and Pavel PLEVKA. Enterovirus particles expel capsid pentamers to enable genome release. \textit{Nature Communications}. London: Nature Publishing Group, 2019, vol.~10, MAR, p.~1-9. ISSN~2041-1723. Available from: https://dx.doi.org/10.1038/s41467-019-09132-x.
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