J 2019

Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1

HARNOŠ, Jakub, M.C.A. CANIZAL, Miroslav JURÁSEK, Jitender KUMAR, Cornelia HOLLER et. al.

Basic information

Original name

Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1

Authors

HARNOŠ, Jakub (203 Czech Republic, belonging to the institution), M.C.A. CANIZAL (276 Germany), Miroslav JURÁSEK (203 Czech Republic, belonging to the institution), Jitender KUMAR (356 India, belonging to the institution), Cornelia HOLLER (276 Germany), Alexandra SCHAMBONY (276 Germany), Kateřina HANÁKOVÁ (203 Czech Republic, belonging to the institution), Ondřej BERNATÍK (203 Czech Republic, belonging to the institution), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution), Kristína GÖMÖRYOVÁ (703 Slovakia, belonging to the institution), Tomáš GYBEĽ (703 Slovakia, belonging to the institution), Tomasz Witold RADASZKIEWICZ (616 Poland, belonging to the institution), Marek KRAVEC (703 Slovakia, belonging to the institution), Lukáš TRANTÍREK (203 Czech Republic, belonging to the institution), Jan RYNEŠ (203 Czech Republic, belonging to the institution), Zankruti DAVE (356 India, belonging to the institution), Ana Iris FERNANDEZ-LLAMAZARES (528 Netherlands), Robert VÁCHA (203 Czech Republic, belonging to the institution), Konstantinos TRIPSIANES (300 Greece, belonging to the institution), Carsten HOFFMANN (276 Germany) and Vítězslav BRYJA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Nature Communications, London, Nature Publishing Group, 2019, 2041-1723

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 12.121

RIV identification code

RIV/00216224:14310/19:00107391

Organization unit

Faculty of Science

DOI

UT WoS

000464979000002

Keywords in English

PARTICLE MESH EWALD; BETA-CATENIN; WNT/BETA-CATENIN; DIX DOMAIN; MOLECULAR-DYNAMICS; STRUCTURAL BASIS; PDZ DOMAIN; DEP DOMAIN; G-PROTEIN; WNT

Tags

Tags

International impact, Reviewed
Změněno: 14/10/2024 13:05, Ing. Jana Kuchtová

Abstract

V originále

Dishevelled (DVL) is the key component of the Wnt signaling pathway. Currently, DVL conformational dynamics under native conditions is unknown. To overcome this limitation, we develop the Fluorescein Arsenical Hairpin Binder- (FlAsH-) based FRET in vivo approach to study DVL conformation in living cells. Using this single-cell FRET approach, we demonstrate that (i) Wnt ligands induce open DVL conformation, (ii) DVL variants that are predominantly open, show more even subcellular localization and more efficient membrane recruitment by Frizzled (FZD) and (iii) Casein kinase 1 epsilon (CK1 epsilon) has a key regulatory function in DVL conformational dynamics. In silico modeling and in vitro biophysical methods explain how CK1 epsilon-specific phosphorylation events control DVL conformations via modulation of the PDZ domain and its interaction with DVL C-terminus. In summary, our study describes an experimental tool for DVL conformational sampling in living cells and elucidates the essential regulatory role of CK1 epsilon in DVL conformational dynamics.

Links

EF16_025/0007381, research and development project
Name: Preklinická progrese nových organických sloučenin s cílenou biologickou aktivitou
GA15-21789S, research and development project
Name: Dishevelled - identifikace základních koordinát
Investor: Czech Science Foundation
GA18-17658S, research and development project
Name: Odhalení tajemství signální dráhy WNT analýzou struktury proteinu Dishevelled
Investor: Czech Science Foundation
GX19-26041X, research and development project
Name: Strukturní biologie nové generace: Od izolovaných molekul k buňkám, od buněk ke tkáním.
Investor: Czech Science Foundation
LM2015085, research and development project
Name: CERIT Scientific Cloud (Acronym: CERIT-SC)
Investor: Ministry of Education, Youth and Sports of the CR, CERIT Scientific Cloud
LQ1601, research and development project
Name: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
MUNI/E/0533/2018, interní kód MU
Name: Studium konformační dynamiky proteinu Dishevelled 3 pomocí FRET sensorů: objev klíčové role Kasein kinázy 1 (CK1). (Acronym: Analýza konformace proteinu Dishevelled 3)
Investor: Masaryk University, Promoting quality excellence
MUNI/G/0739/2017, interní kód MU
Name: Pushing the limits in automated NMR structure determination using a single 4D NOESY spectrum and machine learning methods
Investor: Masaryk University, INTERDISCIPLINARY - Interdisciplinary research projects
MUNI/G/1100/2016, interní kód MU
Name: Computational chemistry for Wnt signaling pathway
Investor: Masaryk University, INTERDISCIPLINARY - Interdisciplinary research projects
NV15-34405A, research and development project
Name: Identifikace nových možností léčby chronické myeloidní leukémie pomocí systematické analýzy interaktomu proteinu BCR-ABL
608180, interní kód MU
Name: WNT-mediated signal relay in stem cells and oncogenesis - from basic biology to applications (Acronym: WntsApp)
Investor: European Union, WNT-mediated signal relay in stem cells and oncogenesis - from basic biology to applications, People
90043, large research infrastructures
Name: CIISB