| HARNOŠ, Jakub, M.C.A. CANIZAL, Miroslav JURÁSEK, Jitender KUMAR, Cornelia HOLLER, Alexandra SCHAMBONY, Kateřina HANÁKOVÁ, Ondřej BERNATÍK, Zbyněk ZDRÁHAL, Kristína GÖMÖRYOVÁ, Tomáš GYBEĽ, Tomasz Witold RADASZKIEWICZ, Marek KRAVEC, Lukáš TRANTÍREK, Jan RYNEŠ, Zankruti DAVE, Ana Iris FERNANDEZ-LLAMAZARES, Robert VÁCHA, Konstantinos TRIPSIANES, Carsten HOFFMANN and Vítězslav BRYJA. Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1. Nature Communications. London: Nature Publishing Group, 2019, vol. 10, APR 18 2019, p. 1-18. ISSN 2041-1723. Available from: https://dx.doi.org/10.1038/s41467-019-09651-7. |
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@article{1529116,
author = {Harnoš, Jakub and Canizal, M.C.A. and Jurásek, Miroslav and Kumar, Jitender and Holler, Cornelia and Schambony, Alexandra and Hanáková, Kateřina and Bernatík, Ondřej and Zdráhal, Zbyněk and Gömöryová, Kristína and Gybeľ, Tomáš and Radaszkiewicz, Tomasz Witold and Kravec, Marek and Trantírek, Lukáš and Ryneš, Jan and Dave, Zankruti and FernandezandLlamazares, Ana Iris and Vácha, Robert and Tripsianes, Konstantinos and Hoffmann, Carsten and Bryja, Vítězslav},
article_location = {London},
article_number = {APR 18 2019},
doi = {http://dx.doi.org/10.1038/s41467-019-09651-7},
keywords = {PARTICLE MESH EWALD; BETA-CATENIN; WNT/BETA-CATENIN; DIX DOMAIN; MOLECULAR-DYNAMICS; STRUCTURAL BASIS; PDZ DOMAIN; DEP DOMAIN; G-PROTEIN; WNT},
language = {eng},
issn = {2041-1723},
journal = {Nature Communications},
title = {Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1},
url = {https://www.nature.com/articles/s41467-019-09651-7},
volume = {10},
year = {2019}
}
TY - JOUR
ID - 1529116
AU - Harnoš, Jakub - Canizal, M.C.A. - Jurásek, Miroslav - Kumar, Jitender - Holler, Cornelia - Schambony, Alexandra - Hanáková, Kateřina - Bernatík, Ondřej - Zdráhal, Zbyněk - Gömöryová, Kristína - Gybeľ, Tomáš - Radaszkiewicz, Tomasz Witold - Kravec, Marek - Trantírek, Lukáš - Ryneš, Jan - Dave, Zankruti - Fernandez-Llamazares, Ana Iris - Vácha, Robert - Tripsianes, Konstantinos - Hoffmann, Carsten - Bryja, Vítězslav
PY - 2019
TI - Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1
JF - Nature Communications
VL - 10
IS - APR 18 2019
SP - 1-18
EP - 1-18
PB - Nature Publishing Group
SN - 20411723
KW - PARTICLE MESH EWALD
KW - BETA-CATENIN
KW - WNT/BETA-CATENIN
KW - DIX DOMAIN
KW - MOLECULAR-DYNAMICS
KW - STRUCTURAL BASIS
KW - PDZ DOMAIN
KW - DEP DOMAIN
KW - G-PROTEIN
KW - WNT
UR - https://www.nature.com/articles/s41467-019-09651-7
L2 - https://www.nature.com/articles/s41467-019-09651-7
N2 - Dishevelled (DVL) is the key component of the Wnt signaling pathway. Currently, DVL conformational dynamics under native conditions is unknown. To overcome this limitation, we develop the Fluorescein Arsenical Hairpin Binder- (FlAsH-) based FRET in vivo approach to study DVL conformation in living cells. Using this single-cell FRET approach, we demonstrate that (i) Wnt ligands induce open DVL conformation, (ii) DVL variants that are predominantly open, show more even subcellular localization and more efficient membrane recruitment by Frizzled (FZD) and (iii) Casein kinase 1 epsilon (CK1 epsilon) has a key regulatory function in DVL conformational dynamics. In silico modeling and in vitro biophysical methods explain how CK1 epsilon-specific phosphorylation events control DVL conformations via modulation of the PDZ domain and its interaction with DVL C-terminus. In summary, our study describes an experimental tool for DVL conformational sampling in living cells and elucidates the essential regulatory role of CK1 epsilon in DVL conformational dynamics.
ER -
HARNOŠ, Jakub, M.C.A. CANIZAL, Miroslav JURÁSEK, Jitender KUMAR, Cornelia HOLLER, Alexandra SCHAMBONY, Kateřina HANÁKOVÁ, Ondřej BERNATÍK, Zbyněk ZDRÁHAL, Kristína GÖMÖRYOVÁ, Tomáš GYBEĽ, Tomasz Witold RADASZKIEWICZ, Marek KRAVEC, Lukáš TRANTÍREK, Jan RYNEŠ, Zankruti DAVE, Ana Iris FERNANDEZ-LLAMAZARES, Robert VÁCHA, Konstantinos TRIPSIANES, Carsten HOFFMANN and Vítězslav BRYJA. Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1. \textit{Nature Communications}. London: Nature Publishing Group, 2019, vol.~10, APR 18 2019, p.~1-18. ISSN~2041-1723. Available from: https://dx.doi.org/10.1038/s41467-019-09651-7.
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