Genes responsible for proliferation, differentiation, and
junction adhesion are significantly up-regulated in human
ovarian granulosa cells during a long-term primary in vitro
culture

J 2019

Genes responsible for proliferation, differentiation, and junction adhesion are significantly up-regulated in human ovarian granulosa cells during a long-term primary in vitro culture

KRANC, Wieslawa, Maciej BRAZERT, Joanna BUDNA, Piotr CELICHOWSKI, Artur BRYJA et. al.

Basic information

Original name

Genes responsible for proliferation, differentiation, and junction adhesion are significantly up-regulated in human ovarian granulosa cells during a long-term primary in vitro culture

Authors

KRANC, Wieslawa (616 Poland), Maciej BRAZERT (616 Poland), Joanna BUDNA (616 Poland), Piotr CELICHOWSKI (616 Poland), Artur BRYJA (616 Poland), Mariusz J. NAWROCKI (616 Poland), Katarzyna OZEGOWSKA (616 Poland), Maurycy JANKOWSKI (616 Poland), Blazej CHERMULA (616 Poland), Marta DYSZKIEWICZ-KONWINSKA (616 Poland), Michal JEŠETA (203 Czech Republic, belonging to the institution), Leszek PAWELCZYK (616 Poland), Andrzej BREBOROWICZ (616 Poland), Dominik RACHON (616 Poland), Malgorzata BRUSKA (616 Poland), Michal NOWICKI (616 Poland), Maciej ZABEL (616 Poland) and Bartosz KEMPISTY (616 Poland, guarantor)

Edition

Histochemistry and Cell Biology, Heidelberg, Springer, 2019, 0948-6143

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30214 Obstetrics and gynaecology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.418

RIV identification code

RIV/00216224:14110/19:00109659

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s00418-018-1750-1

UT WoS

000459058400004

Keywords in English

Granulosa cells; Proliferation; Differentiation; Stem cells; Microarrays

Abstract

V originále

The human ovarian granulosa cells (GCs) surround the oocyte and form the proper architecture of the ovarian follicle. The ability of GCs to proliferate and differentiate in the conditions of in vitro culture has been proven. However, there is still a large field for extensive investigation of molecular basics, as well as marker genes, responsible for these processes. This study aimed to find the new marker genes, encoding proteins that regulate human GCs in vitro capability for proliferation and differentiation during long-term primary culture. The human follicular GCs were collected from hyper-stimulated ovarian follicles during IVF procedures and transferred to a long-term in vitro culture. The culture lasted for 30 days, with RNA samples isolated at days 1, 7, 15, 30. Transcriptomic analysis was then performed with the use of Affymetrix microarray. Obtained results were then subjected to bioinformatical evaluation and sorting. After subjecting the datasets to KEGG analysis, three differentially expressed ontology groups cell differentiation (GO:0030154), cell proliferation (GO:0008283) and cell-cell junction organization (GO:0045216) were chosen for further investigation. All three of those ontology groups are involved in human GCs' in vitro lifespan, proliferation potential, and survival capability. Changes in expression of genes of interest belonging to the chosen GOs were validated with the use of RT-qPCR. In this manuscript, we suggest that VCL, PARVA, FZD2, NCS1, and COL5A1 may be recognized as new markers of GC in vitro differentiation, while KAT2B may be a new marker of their proliferation. Additionally, SKI, GLI2, FERMT2, and CDH2 could also be involved in GC in vitro proliferation and differentiation processes. We demonstrated that, in long-term in vitro culture, GCs exhibit markers that suggest their ability to differentiate into different cells types. Therefore, the higher expression profile of these genes may also be associated with the induction of cellular differentiation processes that take place beyond the long-term primary in vitro culture.

Citovat

KRANC, Wieslawa, Maciej BRAZERT, Joanna BUDNA, Piotr CELICHOWSKI, Artur BRYJA, Mariusz J. NAWROCKI, Katarzyna OZEGOWSKA, Maurycy JANKOWSKI, Blazej CHERMULA, Marta DYSZKIEWICZ-KONWINSKA, Michal JEŠETA, Leszek PAWELCZYK, Andrzej BREBOROWICZ, Dominik RACHON, Malgorzata BRUSKA, Michal NOWICKI, Maciej ZABEL and Bartosz KEMPISTY. Genes responsible for proliferation, differentiation, and junction adhesion are significantly up-regulated in human ovarian granulosa cells during a long-term primary in vitro culture. Histochemistry and Cell Biology. Heidelberg: Springer, 2019, vol. 151, No 2, p. 125-143. ISSN 0948-6143. Available from: https://dx.doi.org/10.1007/s00418-018-1750-1.

@article{1531296,
   author = {Kranc, Wieslawa and Brazert, Maciej and Budna, Joanna and Celichowski, Piotr and Bryja, Artur and Nawrocki, Mariusz J. and Ozegowska, Katarzyna and Jankowski, Maurycy and Chermula, Blazej and DyszkiewiczandKonwinska, Marta and Ješeta, Michal and Pawelczyk, Leszek and Breborowicz, Andrzej and Rachon, Dominik and Bruska, Malgorzata and Nowicki, Michal and Zabel, Maciej and Kempisty, Bartosz},
   article_location = {Heidelberg},
   article_number = {2},
   doi = {http://dx.doi.org/10.1007/s00418-018-1750-1},
   keywords = {Granulosa cells; Proliferation; Differentiation; Stem cells; Microarrays},
   language = {eng},
   issn = {0948-6143},
   journal = {Histochemistry and Cell Biology},
   title = {Genes responsible for proliferation, differentiation, and junction adhesion are significantly up-regulated in human ovarian granulosa cells during a long-term primary in vitro culture},
   url = {http://dx.doi.org/10.1007/s00418-018-1750-1},
   volume = {151},
   year = {2019}
}

TY  - JOUR
ID  - 1531296
AU  - Kranc, Wieslawa - Brazert, Maciej - Budna, Joanna - Celichowski, Piotr - Bryja, Artur - Nawrocki, Mariusz J. - Ozegowska, Katarzyna - Jankowski, Maurycy - Chermula, Blazej - Dyszkiewicz-Konwinska, Marta - Ješeta, Michal - Pawelczyk, Leszek - Breborowicz, Andrzej - Rachon, Dominik - Bruska, Malgorzata - Nowicki, Michal - Zabel, Maciej - Kempisty, Bartosz
PY  - 2019
TI  - Genes responsible for proliferation, differentiation, and junction adhesion are significantly up-regulated in human ovarian granulosa cells during a long-term primary in vitro culture
JF  - Histochemistry and Cell Biology
VL  - 151
IS  - 2
SP  - 125-143
EP  - 125-143
PB  - Springer
SN  - 09486143
KW  - Granulosa cells
KW  - Proliferation
KW  - Differentiation
KW  - Stem cells
KW  - Microarrays
UR  - http://dx.doi.org/10.1007/s00418-018-1750-1
L2  - http://dx.doi.org/10.1007/s00418-018-1750-1
N2  - The human ovarian granulosa cells (GCs) surround the oocyte and form the proper architecture of the ovarian follicle. The ability of GCs to proliferate and differentiate in the conditions of in vitro culture has been proven. However, there is still a large field for extensive investigation of molecular basics, as well as marker genes, responsible for these processes. This study aimed to find the new marker genes, encoding proteins that regulate human GCs in vitro capability for proliferation and differentiation during long-term primary culture. The human follicular GCs were collected from hyper-stimulated ovarian follicles during IVF procedures and transferred to a long-term in vitro culture. The culture lasted for 30 days, with RNA samples isolated at days 1, 7, 15, 30. Transcriptomic analysis was then performed with the use of Affymetrix microarray. Obtained results were then subjected to bioinformatical evaluation and sorting. After subjecting the datasets to KEGG analysis, three differentially expressed ontology groups cell differentiation (GO:0030154), cell proliferation (GO:0008283) and cell-cell junction organization (GO:0045216) were chosen for further investigation. All three of those ontology groups are involved in human GCs' in vitro lifespan, proliferation potential, and survival capability. Changes in expression of genes of interest belonging to the chosen GOs were validated with the use of RT-qPCR. In this manuscript, we suggest that VCL, PARVA, FZD2, NCS1, and COL5A1 may be recognized as new markers of GC in vitro differentiation, while KAT2B may be a new marker of their proliferation. Additionally, SKI, GLI2, FERMT2, and CDH2 could also be involved in GC in vitro proliferation and differentiation processes. We demonstrated that, in long-term in vitro culture, GCs exhibit markers that suggest their ability to differentiate into different cells types. Therefore, the higher expression profile of these genes may also be associated with the induction of cellular differentiation processes that take place beyond the long-term primary in vitro culture.
ER  -

KRANC, Wieslawa, Maciej BRAZERT, Joanna BUDNA, Piotr CELICHOWSKI, Artur BRYJA, Mariusz J. NAWROCKI, Katarzyna OZEGOWSKA, Maurycy JANKOWSKI, Blazej CHERMULA, Marta DYSZKIEWICZ-KONWINSKA, Michal JEŠETA, Leszek PAWELCZYK, Andrzej BREBOROWICZ, Dominik RACHON, Malgorzata BRUSKA, Michal NOWICKI, Maciej ZABEL and Bartosz KEMPISTY. Genes responsible for proliferation, differentiation, and junction adhesion are significantly up-regulated in human ovarian granulosa cells during a long-term primary in vitro culture. \textit{Histochemistry and Cell Biology}. Heidelberg: Springer, 2019, vol.~151, No~2, p.~125-143. ISSN~0948-6143. Available from: https://dx.doi.org/10.1007/s00418-018-1750-1.

Detailed Information on Publication Record