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@article{1531300,
author = {Záveský, Luděk and Jandáková, Eva and Weinberger, Vít and Minář, Luboš and Hanzíková, Veronika and Dušková, Daniela and Záveská Drábková, Lenka and Svobodová, Iveta and Hořínek, Aleš},
article_location = {THOUSAND OAKS},
article_number = {4},
doi = {http://dx.doi.org/10.1177/1933719118776808},
keywords = {ovarian cancer; ascites; microRNA; diagnostic markers; prognostic markers},
language = {eng},
issn = {1933-7191},
journal = {REPRODUCTIVE SCIENCES},
title = {Ascites-Derived Extracellular microRNAs as Potential Biomarkers for Ovarian Cancer},
url = {http://dx.doi.org/10.1177/1933719118776808},
volume = {26},
year = {2019}
}
TY - JOUR
ID - 1531300
AU - Záveský, Luděk - Jandáková, Eva - Weinberger, Vít - Minář, Luboš - Hanzíková, Veronika - Dušková, Daniela - Záveská Drábková, Lenka - Svobodová, Iveta - Hořínek, Aleš
PY - 2019
TI - Ascites-Derived Extracellular microRNAs as Potential Biomarkers for Ovarian Cancer
JF - REPRODUCTIVE SCIENCES
VL - 26
IS - 4
SP - 510-522
EP - 510-522
PB - SAGE PUBLICATIONS INC
SN - 19337191
KW - ovarian cancer
KW - ascites
KW - microRNA
KW - diagnostic markers
KW - prognostic markers
UR - http://dx.doi.org/10.1177/1933719118776808
L2 - http://dx.doi.org/10.1177/1933719118776808
N2 - Ovarian cancer as the most fatal gynecological malignancy is often manifested by excessive fluid accumulation known as ascites or effusion. Ascites-derived microRNAs (miRNAs) may be closely associated with ovarian cancer progression. However, our knowledge of their roles, altered expression, and clinical outcomes remained limited. In this study, large-scale expression profiling of 754 human miRNAs was performed using real-time quantitative polymerase chain reaction and 384-well TaqMan array human miRNA A and B cards to identify differentially expressed miRNAs between extracellular fraction of the ascitic fluid associated with high-grade serous ovarian carcinomas and control plasma. Of the 754 miRNAs, 153 were significantly differentially expressed relative to the controls. Expression of 7 individual miRNAs (miR-200a, miR-200b, miR-200c, miR-141, miR-429, miR-1290, and miR-30a-5p) was further validated in extended sample sets, including serous, endometrioid, and mucinous subtypes. All miR-200 family members and miR-1290 were conspicuously overexpressed, while miR-30a-5p was only weakly overexpressed. The ability of miRNAs expression to discriminate the pathological samples from the controls was strong. Receiver operating characteristic curve analyses found area under the curve (AUC) values of 1.000 for miR-200a, miR-200c, miR-141, miR-429, and miR-1290 and of AUC 0.996 and 0.885 for miR-200b and miR-30a-5p, respectively. Preliminary survival analyses indicated low expression level of miR-200b as significantly related to longer overall survival (hazard ratio [HR]: 0.25, mean survival 44 months), while high expression level was related to poor overall survival (HR: 4.04, mean survival 24 months). Our findings suggested that ascites-derived miRNAs should be further explored and evaluated as potential diagnostic and prognostic biomarkers for ovarian cancer.
ER -
ZÁVESKÝ, Luděk, Eva JANDÁKOVÁ, Vít WEINBERGER, Luboš MINÁŘ, Veronika HANZÍKOVÁ, Daniela DUŠKOVÁ, Lenka ZÁVESKÁ DRÁBKOVÁ, Iveta SVOBODOVÁ and Aleš HOŘÍNEK. Ascites-Derived Extracellular microRNAs as Potential Biomarkers for Ovarian Cancer. \textit{REPRODUCTIVE SCIENCES}. THOUSAND OAKS: SAGE PUBLICATIONS INC, vol.~26, No~4, p.~510-522. ISSN~1933-7191. doi:10.1177/1933719118776808. 2019.
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