J 2019

Fibroblast growth factor receptor influences primary cilium length through an interaction with intestinal cell kinase

BOSÁKOVÁ, Michaela, Alexandru NITĂ, Tomáš GREGOR, Miroslav VAŘECHA, Iva GUDERNOVÁ et. al.

Basic information

Original name

Fibroblast growth factor receptor influences primary cilium length through an interaction with intestinal cell kinase

Authors

BOSÁKOVÁ, Michaela (203 Czech Republic, belonging to the institution), Alexandru NITĂ (642 Romania, belonging to the institution), Tomáš GREGOR (203 Czech Republic, belonging to the institution), Miroslav VAŘECHA (203 Czech Republic, belonging to the institution), Iva GUDERNOVÁ (203 Czech Republic, belonging to the institution), Bohumil FAFÍLEK (203 Czech Republic, belonging to the institution), Tomáš BÁRTA (203 Czech Republic, belonging to the institution), Neha BASHEER (356 India, belonging to the institution), Sara POOVAKULATHU ABRAHAM (356 India, belonging to the institution), Lukáš BÁLEK (203 Czech Republic, belonging to the institution), Markéta TOMANOVÁ (203 Czech Republic, belonging to the institution), Jana FIALOVÁ KUČEROVÁ (203 Czech Republic, belonging to the institution), Juraj BOSÁK (703 Slovakia, belonging to the institution), David POTĚŠIL (203 Czech Republic, belonging to the institution), Jennifer ZIEBA (840 United States of America), Jieun SONG (410 Republic of Korea), Peter KONIK (203 Czech Republic), Sohyun PARK (840 United States of America), Ivan DURAN (840 United States of America), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution), David ŠMAJS (203 Czech Republic, belonging to the institution), Gert JANSEN (528 Netherlands), Zheng FU (840 United States of America), Hyuk Wan KO (410 Republic of Korea), Aleš HAMPL (203 Czech Republic, belonging to the institution), Lukáš TRANTÍREK (203 Czech Republic, belonging to the institution), Deborah KRAKOW (840 United States of America) and Pavel KREJČÍ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Proceedings of the National Academy of Sciences of the United States of America, WASHINGTON, NATL ACAD SCIENCES, 2019, 0027-8424

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10602 Biology , Evolutionary biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 9.412

RIV identification code

RIV/00216224:14110/19:00107404

Organization unit

Faculty of Medicine

DOI

UT WoS

000460242100061

Keywords in English

fibroblast growth factor; FGFR; intestinal cell kinase; ICK; cilia length

Tags

Tags

International impact, Reviewed
Změněno: 9/10/2024 14:06, Ing. Martina Blahová

Abstract

V originále

Vertebrate primary cilium is a Hedgehog signaling center but the extent of its involvement in other signaling systems is less well understood. This report delineates a mechanism by which fibroblast growth factor (FGF) controls primary cilia. Employing proteomic approaches to characterize proteins associated with the FGF-receptor, FGFR3, we identified the serine/threonine kinase intestinal cell kinase (ICK) as an FGFR interactor. ICK is involved in ciliogenesis and participates in control of ciliary length. FGF signaling partially abolished ICK's kinase activity, through FGFR-mediated ICK phosphorylation at conserved residue Tyr15, which interfered with optimal ATP binding. Activation of the FGF signaling pathway affected both primary cilia length and function in a manner consistent with cilia effects caused by inhibition of ICK activity. Moreover, knockdown and knockout of ICK rescued the FGF-mediated effect on cilia. We provide conclusive evidence that FGF signaling controls cilia via interaction with ICK.

Links

GA17-09525S, research and development project
Name: Neobvyklé signální dráhy lidských receptorových tyrozinových kináz
Investor: Czech Science Foundation
GJ16-24004Y, research and development project
Name: Indukce buněčné plasticity prostřednictvím modulace mikroRNA molekul: Nový přístup pro přeprogramování buněk
Investor: Czech Science Foundation
LH15231, research and development project
Name: Nové mechanismy vzniku fatálních kostních ciliopatií u člověka
Investor: Ministry of Education, Youth and Sports of the CR
LM2015043, research and development project
Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)
Investor: Ministry of Education, Youth and Sports of the CR
LQ1601, research and development project
Name: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
MUNI/A/1087/2018, interní kód MU
Name: Molekulární a buněčná biologie pro biomedicínské vědy
Investor: Masaryk University, Category A
MUNI/E/0563/2018, interní kód MU
Name: Fibroblast Growth Factor Signaling Controls Primary Cilia Length via Intestinal Cell Kinase (Acronym: FGF controls cilia via ICK)
Investor: Masaryk University, Promoting quality excellence
NV15-33232A, research and development project
Name: Identifikace nových možností léčby achondroplásie prostřednictvím analýzy interakce FGFR3 a adaptérového proteinu Frs2
NV15-34405A, research and development project
Name: Identifikace nových možností léčby chronické myeloidní leukémie pomocí systematické analýzy interaktomu proteinu BCR-ABL
ROZV/24/LF/2018, interní kód MU
Name: LF - Příspěvek na IP 2108
Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects
90043, large research infrastructures
Name: CIISB