Detailed Information on Publication Record
2019
The Accumulation and Effects of Liposomal Doxorubicin in Tissues Treated by Radiofrequency Ablation and Irreversible Electroporation in Liver: In Vivo Experimental Study on Porcine Models
ANDRAŠINA, Tomáš, Josef JAROŠ, Tomáš JŮZA, Tomáš ROHAN, Dalibor ČERVINKA et. al.Basic information
Original name
The Accumulation and Effects of Liposomal Doxorubicin in Tissues Treated by Radiofrequency Ablation and Irreversible Electroporation in Liver: In Vivo Experimental Study on Porcine Models
Authors
ANDRAŠINA, Tomáš (703 Slovakia, belonging to the institution), Josef JAROŠ (203 Czech Republic, belonging to the institution), Tomáš JŮZA (203 Czech Republic, guarantor, belonging to the institution), Tomáš ROHAN (203 Czech Republic, belonging to the institution), Dalibor ČERVINKA (203 Czech Republic), Michal CRHA (203 Czech Republic), Vlastimil VÁLEK (203 Czech Republic, belonging to the institution) and Nahum S. GOLDBERG (376 Israel)
Edition
Cardiovascular and Interventional Radiology, NEW YORK, SPRINGER, 2019, 0174-1551
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30224 Radiology, nuclear medicine and medical imaging
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 2.034
RIV identification code
RIV/00216224:14110/19:00108469
Organization unit
Faculty of Medicine
UT WoS
000462586800014
Keywords in English
Liposomal doxorubicin; Radiofrequency ablation; Irreversible electroporation
Tags
International impact, Reviewed
Změněno: 31/1/2020 16:22, Mgr. Tereza Miškechová
Abstract
V originále
PurposeTo compare the accumulation and effect of liposomal doxorubicin in liver tissue treated by radiofrequency ablation (RFA) and irreversible electroporation (IRE) in in vivo porcine models.Materials and MethodsSixteen RFA and 16 IRE procedures were performed in healthy liver of two groups of three pigs. Multi-tined RFA parameters included: 100W, target temperature 105 degrees C for 7min. 100 IRE pulses were delivered using two monopolar electrodes at 2250V, 1Hz, for 100 mu sec. For each group, two pigs received 50mg liposomal doxorubicin (0.5mg/kg) as a drip infusion during ablation procedure, with one pig serving as control. Samples were harvested from the central and peripheral zones of the ablation at 24 and 72h. Immunohistochemical analysis to evaluate the degree of cellular stress, DNA damage, and degree of apoptosis was performed. These and the ablation sizes were compared. Doxorubicin concentrations were also analyzed using fluorescence photometry of homogenized tissue.ResultsRFA treatment zones created with concomitant administration of doxorubicin at 24h were significantly larger than controls (2.50.3cm vs. 2.2 +/- 0.2cm; p=0.04). By contrast, IRE treatment zones were negatively influenced by chemotherapy (2.2 +/- 0.4cm vs. 2.6 +/- 0.4cm; p=0.05). At 24h, doxorubicin concentrations in peripheral and central zones of RFA were significantly increased in comparison with untreated parenchyma (0.431 +/- 0.078 mu g/g and 0.314 +/- 0.055 mu g/g vs. 0.18 +/- 0.012 mu g/g; p<0.05). Doxorubicin concentrations in IRE zones were not significantly different from untreated liver (0.191 +/- 0.049 mu g/g and 0.210 +/- 0.049 mu g/g vs. 0.18 +/- 0.012 mu g/g).Conclusionsp id=Par4Whereas there is an increased accumulation of periprocedural doxorubicin and an associated increase in ablation zone following RFA, a contrary effect is noted with IRE. These discrepant findings suggest that different mechanisms and synergies will need to be considered in order to select optimal adjuvants for different classes of ablation devices.
Links
| MUNI/A/1565/2018, interní kód MU |
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| NV15-32484A, research and development project |
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| ROZV/24/LF/2018, interní kód MU |
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