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@article{1531658,
author = {Obrucova, H. and Kotásková, Iva and Tihelkova, R. and Holá, Veronika and Růžička, Filip and Freiberger, Tomáš},
article_location = {Washington},
article_number = {4},
doi = {http://dx.doi.org/10.1128/JCM.01861-18},
keywords = {Candida; biofilms; capillary electrophoresis; fungi; panfungal PCR; polyfungal sample; ureteral stents; urinary catheters},
language = {eng},
issn = {0095-1137},
journal = {Journal of Clinical Microbiology},
title = {Fluorescent Capillary Electrophoresis Is Superior to Culture in Detecting Candida Species from Samples of Urinary Catheters and Ureteral Stents with Mono- or Polyfungal Biofilm Growth},
url = {https://jcm.asm.org/content/57/4/e01861-18},
volume = {57},
year = {2019}
}
TY - JOUR
ID - 1531658
AU - Obrucova, H. - Kotásková, Iva - Tihelkova, R. - Holá, Veronika - Růžička, Filip - Freiberger, Tomáš
PY - 2019
TI - Fluorescent Capillary Electrophoresis Is Superior to Culture in Detecting Candida Species from Samples of Urinary Catheters and Ureteral Stents with Mono- or Polyfungal Biofilm Growth
JF - Journal of Clinical Microbiology
VL - 57
IS - 4
SP - nestrankovano
EP - nestrankovano
PB - American Society for Microbiology
SN - 00951137
KW - Candida
KW - biofilms
KW - capillary electrophoresis
KW - fungi
KW - panfungal PCR
KW - polyfungal sample
KW - ureteral stents
KW - urinary catheters
UR - https://jcm.asm.org/content/57/4/e01861-18
L2 - https://jcm.asm.org/content/57/4/e01861-18
N2 - Molecular techniques in fungal detection and identification represent an efficient complementary diagnostic tool which is increasingly used to overcome limitations of routinely used culture techniques. The aim of this study was to characterize Candida sp. representation in samples from urine, urinary catheter, and ureteral stent biofilm using ITS2 ribosomal DNA (rDNA) amplification followed by fluorescent capillary electrophoresis (f-ITS2-PCR-CE) and to compare the results with those obtained by culture. A total of 419 samples were analyzed, and 106 (25.2%) were found positive, out of which 17 (16%) were polyfungal. The positivity rate did not differ between samples from catheters and stents (23.6% versus 20.9%) or between catheter and stent corresponding urine samples (40.2% versus 30.2%). Ten different Candida species were detected, with Candida parapsilosis (31.4%), Candida albicans (26.5%), and Candida tropicalis (12.4%) predominating. f-ITS2-PCR-CE was evaluated as substantially less time-consuming and 8.3 times more sensitive than the routinely applied culture technique with 1 mu l of urine/sonicated fluid inoculated, detecting 67 (19.9%) versus 8 (2.4%) positive samples out of 337 initially analyzed samples. The culture sensitivity considerably improved to 1.7 times lower than that of f-ITS2-PCR-CE after the inoculation volume was increased to 100 mu l in the additional 82 samples. Moreover, the molecular technique, unlike routine cultivation, enabled precise pathogen composition determination in polymicrobial samples. In conclusion, the f-ITS2-PCR-CE method was shown to be a quick and efficient tool for culture-independent detection and identification of fungi in urinary tract-related samples, demonstrating a higher sensitivity than culture.
ER -
OBRUCOVA, H., Iva KOTÁSKOVÁ, R. TIHELKOVA, Veronika HOLÁ, Filip RŮŽIČKA and Tomáš FREIBERGER. Fluorescent Capillary Electrophoresis Is Superior to Culture in Detecting Candida Species from Samples of Urinary Catheters and Ureteral Stents with Mono- or Polyfungal Biofilm Growth. \textit{Journal of Clinical Microbiology}. Washington: American Society for Microbiology, 2019, vol.~57, No~4, p.~nestrankovano, 10 pp. ISSN~0095-1137. Available from: https://dx.doi.org/10.1128/JCM.01861-18.
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