J 2019

Fluorescent Capillary Electrophoresis Is Superior to Culture in Detecting Candida Species from Samples of Urinary Catheters and Ureteral Stents with Mono- or Polyfungal Biofilm Growth

OBRUCOVA, H., Iva KOTÁSKOVÁ, R. TIHELKOVA, Veronika HOLÁ, Filip RŮŽIČKA et. al.

Basic information

Original name

Fluorescent Capillary Electrophoresis Is Superior to Culture in Detecting Candida Species from Samples of Urinary Catheters and Ureteral Stents with Mono- or Polyfungal Biofilm Growth

Authors

OBRUCOVA, H. (203 Czech Republic), Iva KOTÁSKOVÁ (203 Czech Republic, belonging to the institution), R. TIHELKOVA (203 Czech Republic), Veronika HOLÁ (203 Czech Republic, belonging to the institution), Filip RŮŽIČKA (203 Czech Republic, belonging to the institution) and Tomáš FREIBERGER (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of Clinical Microbiology, Washington, American Society for Microbiology, 2019, 0095-1137

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 5.897

RIV identification code

RIV/00216224:14740/19:00108471

Organization unit

Central European Institute of Technology

DOI

UT WoS

000462714100028

Keywords in English

Candida; biofilms; capillary electrophoresis; fungi; panfungal PCR; polyfungal sample; ureteral stents; urinary catheters

Tags

Tags

International impact, Reviewed
Změněno: 12/5/2020 13:28, Mgr. Pavla Foltynová, Ph.D.

Abstract

V originále

Molecular techniques in fungal detection and identification represent an efficient complementary diagnostic tool which is increasingly used to overcome limitations of routinely used culture techniques. The aim of this study was to characterize Candida sp. representation in samples from urine, urinary catheter, and ureteral stent biofilm using ITS2 ribosomal DNA (rDNA) amplification followed by fluorescent capillary electrophoresis (f-ITS2-PCR-CE) and to compare the results with those obtained by culture. A total of 419 samples were analyzed, and 106 (25.2%) were found positive, out of which 17 (16%) were polyfungal. The positivity rate did not differ between samples from catheters and stents (23.6% versus 20.9%) or between catheter and stent corresponding urine samples (40.2% versus 30.2%). Ten different Candida species were detected, with Candida parapsilosis (31.4%), Candida albicans (26.5%), and Candida tropicalis (12.4%) predominating. f-ITS2-PCR-CE was evaluated as substantially less time-consuming and 8.3 times more sensitive than the routinely applied culture technique with 1 mu l of urine/sonicated fluid inoculated, detecting 67 (19.9%) versus 8 (2.4%) positive samples out of 337 initially analyzed samples. The culture sensitivity considerably improved to 1.7 times lower than that of f-ITS2-PCR-CE after the inoculation volume was increased to 100 mu l in the additional 82 samples. Moreover, the molecular technique, unlike routine cultivation, enabled precise pathogen composition determination in polymicrobial samples. In conclusion, the f-ITS2-PCR-CE method was shown to be a quick and efficient tool for culture-independent detection and identification of fungi in urinary tract-related samples, demonstrating a higher sensitivity than culture.

Links

MUNI/A/0925/2017, interní kód MU
Name: Poruchy tvorby protilátek a komplementového systému
Investor: Masaryk University, Category A
MUNI/A/1189/2018, interní kód MU
Name: Původci nozokomiálních nákaz a možnosti jejich diagnostiky a terapie (Acronym: PNNMDT)
Investor: Masaryk University, Category A
NV16-31593A, research and development project
Name: Polymikrobiální biofilm jako komplexní mikrobiom v rámci léčby nozokomiálních infekcí