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@article{1535338,
author = {Hemzal, Dušan and Kang, Yu Ri and Dvořák, Jan and Kabzinski, Tomasz and Kubíček, Karel and Young Dong, Kim and Humlíček, Josef},
article_location = {Thousand Oaks},
article_number = {3},
doi = {http://dx.doi.org/10.1177/0003702819826280},
keywords = {Spectroscopic ellipsometry; surface plasmon resonance; SPR; total internal reflection ellipsometry; TIRE; RNA polymerase II; C-terminal domain; CTD; Rtt103 protein},
language = {eng},
issn = {0003-7028},
journal = {APPLIED SPECTROSCOPY},
title = {Treatment of Surface Plasmon Resonance (SPR) Background in Total Internal Reflection Ellipsometry: Characterization of RNA Polymerase II Film Formation},
url = {https://journals.sagepub.com/doi/abs/10.1177/0003702819826280?rfr_dat=cr_pub%3Dpubmed&url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&journalCode=aspc},
volume = {73},
year = {2019}
}
TY - JOUR
ID - 1535338
AU - Hemzal, Dušan - Kang, Yu Ri - Dvořák, Jan - Kabzinski, Tomasz - Kubíček, Karel - Young Dong, Kim - Humlíček, Josef
PY - 2019
TI - Treatment of Surface Plasmon Resonance (SPR) Background in Total Internal Reflection Ellipsometry: Characterization of RNA Polymerase II Film Formation
JF - APPLIED SPECTROSCOPY
VL - 73
IS - 3
SP - 261-270
EP - 261-270
PB - SAGE PUBLICATIONS INC
SN - 00037028
KW - Spectroscopic ellipsometry
KW - surface plasmon resonance
KW - SPR
KW - total internal reflection ellipsometry
KW - TIRE
KW - RNA polymerase II
KW - C-terminal domain
KW - CTD
KW - Rtt103 protein
UR - https://journals.sagepub.com/doi/abs/10.1177/0003702819826280?rfr_dat=cr_pub%3Dpubmed&url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&journalCode=aspc
L2 - https://journals.sagepub.com/doi/abs/10.1177/0003702819826280?rfr_dat=cr_pub%3Dpubmed&url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&journalCode=aspc
N2 - To deal with the general problem of biomolecule specific binding analysis, we have applied the technique of difference spectra to the surface plasmon resonance (SPR)-enhanced total internal reflection ellipsometry measurement. We suggest a three-step treatment of the SPR background that can easily be integrated with the usual measurement routine. First, making use of the difference spectrum in ellipsometric angle Delta, single peak footprints of the topmost layer are obtained that facilitate its sensitive detection during film growth. Subsequently, circumventing the need for explicit knowledge of the substrate properties, the difference spectra peaks can be used for the end-point analysis of a binding. Finally, tracking the binding effectivity of the analyte we determine the injection speed and analyte concentration windows needed for successful monitoring of the film growth. We demonstrate our approach on a comprehensive two-stage binding experiment involving two biologically relevant molecules: the C-terminal domain (CTD) of RNA polymerase II and CTD-interacting domain of one of its transcription factors, the Rtt103 protein.
ER -
HEMZAL, Dušan, Yu Ri KANG, Jan DVOŘÁK, Tomasz KABZINSKI, Karel KUBÍČEK, Kim YOUNG DONG and Josef HUMLÍČEK. Treatment of Surface Plasmon Resonance (SPR) Background in Total Internal Reflection Ellipsometry: Characterization of RNA Polymerase II Film Formation. \textit{APPLIED SPECTROSCOPY}. Thousand Oaks: SAGE PUBLICATIONS INC, 2019, vol.~73, No~3, p.~261-270. ISSN~0003-7028. Available from: https://dx.doi.org/10.1177/0003702819826280.
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