Unusual puzzle – retention properties of nucleobases and nucleotides as elements for retention model of ultrashort oligonucleotides

TOMICZKOVÁ, Petra, Miroslava BITTOVÁ and Jan HAVLIŠ. Unusual puzzle – retention properties of nucleobases and nucleotides as elements for retention model of ultrashort oligonucleotides. In Česka chromatografická škola - HPLC 2019. 2019.

Basic information

Original name

Unusual puzzle – retention properties of nucleobases and nucleotides as elements for retention model of ultrashort oligonucleotides

Authors

TOMICZKOVÁ, Petra, Miroslava BITTOVÁ and Jan HAVLIŠ

Edition

Česka chromatografická škola - HPLC 2019, 2019

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Other information

Language

English

Type of outcome

Konferenční abstrakt

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Organization unit

Faculty of Science

Keywords in English

nucleobases, nucleotide monophosphates, ultrashort oligonucleotides, hydrophilic interaction liquid chromatography (HILIC), quantitative structure-retention relationship (QSRR)

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Abstract

V originále

To construct functional model of sequence-dependent separation of ultrashort oligonucleotides in HILIC, we have to consider linear contribution of partial structures (nucleobase, ribose, monophosphate) to overall retention. Within the problem, we consider the nucleobase and phosphate to have the highest impact. To optimally analyse the retention behaviour of ultrashort oligonucleotides in regard to their structure, we have acquired retention factors of individual nucleobases (U, T, A, G a C) and respective nucleotide monophosphates (NMP) under defined conditions, i.e. constant content of organic component (80% ACN), variable pH (1 – 6) and TEAP concentration (20 and 100 mM).