The effect of shock freezing on physiological properties and
consequent growth of Antarctic filamentous (Stigeoclonium sp.)
and coccal alga (Diplosphaera chodatii) on agar plates

J 2019

The effect of shock freezing on physiological properties and consequent growth of Antarctic filamentous (Stigeoclonium sp.) and coccal alga (Diplosphaera chodatii) on agar plates

OREKHOVA, Alla, Miloš BARTÁK, Aleyna OZKAR and Josef ELSTER

Basic information

Original name

The effect of shock freezing on physiological properties and consequent growth of Antarctic filamentous (Stigeoclonium sp.) and coccal alga (Diplosphaera chodatii) on agar plates

Name in Czech

Vliv šokového zmražení na fyziologické vlastnosti a následný růst antarktické vláknité (Stigeoclonium sp.) a kokální řasy (Diplosphaera chodatii)v agarové kultuře

Authors

OREKHOVA, Alla (643 Russian Federation, belonging to the institution), Miloš BARTÁK (203 Czech Republic, guarantor, belonging to the institution), Aleyna OZKAR (792 Turkey) and Josef ELSTER (203 Czech Republic)

Edition

Czech Polar Reports, Masarykova univerzita, 2019, 1805-0689

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Full Text

RIV identification code

RIV/00216224:14310/19:00109872

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.5817/CPR2019-1-4

Keywords (in Czech)

Antarktida; stres chladem; kryoresistence; řasové kultury; biotechnologie

Keywords in English

Antarctica; cold stress; cryoresistance; algal cultures; biotechnologies

Abstract

ORIG CZ

V originále

In this study, we investigated the effects of shock freezing on physiological properties and consequent growth of in the Antarctic alga Stigeoclonium sp. and comparative coccal alga Diplosphaera chodatii on agar plates. Culture of algae grown in liquid medium were used to study subzero temperatures on the species resistance to shock freezing. Then, microalgae were frozen in liquid nitrogen and inoculated on BBM agar after thawing. Physiological status of algae was evaluated by chlorophyll fluorescence parameters during 28 days. The results showed that interspecific differences existed in their tolerance to shock freezing, as well as their consequent growth rate on agars. Direct effects of freezing in liquid nitrogen was demonstrated in chlorophyll fluorescence parameters recorded immediately after thawing the samples (in liquid medium). In spite of the fact that majority of cells was destroyed by shock freezing, the potential of photochemical processes in PS II (FV/FM) remained constant in D. chodatii. It may indicate high resistance of the species to freezing/thawing cycles and a capability of the surviving cells, core chlorophylls in PS II respectively, to perform photosynthetic processes related to PS II. Contrastingly, Stigeoclonium sp. showed a shock freezing-dependent decrease in FV/FM. When shock-frozen, thawed and inoculated on agar plates, the culture of D. chodatii, and Stigeoclonium sp. showed cultivation time-dependent increase in chlorophyll fluorescence parameters (FV/FM, FS).

In Czech

V této studii jsme zkoumali účinky šokového zmrazení na fyziologické vlastnosti a následný růst v antarktické řase Stigeoclonium sp. a srovnávací kokální řase Diplosphaera chodatii na agarových kulturách.

Links

EF16_013/0001708, research and development project

Name: ECOPOLARIS - Změny ve struktuře a funkci součástí terestrických polárních ekosystémů (CzechPolar2)

LM2015078, research and development project

Name: Česká polární výzkumná infrastruktura (Acronym: CzechPolar2)

Investor: Ministry of Education, Youth and Sports of the CR

Files attached

Orekhova_web.pdf

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Citovat

OREKHOVA, Alla, Miloš BARTÁK, Aleyna OZKAR and Josef ELSTER. The effect of shock freezing on physiological properties and consequent growth of Antarctic filamentous (Stigeoclonium sp.) and coccal alga (Diplosphaera chodatii) on agar plates. Czech Polar Reports. Masarykova univerzita, 2019, vol. 9, No 1, p. 37-48. ISSN 1805-0689. Available from: https://dx.doi.org/10.5817/CPR2019-1-4.

@article{1538259,
   author = {Orekhova, Alla and Barták, Miloš and Ozkar, Aleyna and Elster, Josef},
   article_number = {1},
   doi = {http://dx.doi.org/10.5817/CPR2019-1-4},
   keywords = {Antarctica; cold stress; cryoresistance; algal cultures; biotechnologies},
   language = {eng},
   issn = {1805-0689},
   journal = {Czech Polar Reports},
   title = {The effect of shock freezing on physiological properties and consequent growth of Antarctic filamentous (Stigeoclonium sp.) and coccal alga (Diplosphaera chodatii) on agar plates},
   url = {http://www.sci.muni.cz/CPR/17cislo/Orekhova_web.pdf},
   volume = {9},
   year = {2019}
}

TY  - JOUR
ID  - 1538259
AU  - Orekhova, Alla - Barták, Miloš - Ozkar, Aleyna - Elster, Josef
PY  - 2019
TI  - The effect of shock freezing on physiological properties and consequent growth of Antarctic filamentous (Stigeoclonium sp.) and coccal alga (Diplosphaera chodatii) on agar plates
JF  - Czech Polar Reports
VL  - 9
IS  - 1
SP  - 37-48
EP  - 37-48
PB  - Masarykova univerzita
SN  - 18050689
KW  - Antarctica
KW  - cold stress
KW  - cryoresistance
KW  - algal cultures
KW  - biotechnologies
UR  - http://www.sci.muni.cz/CPR/17cislo/Orekhova_web.pdf
L2  - http://www.sci.muni.cz/CPR/17cislo/Orekhova_web.pdf
N2  - In this study, we investigated the effects of shock freezing on physiological properties and consequent growth of in the Antarctic alga Stigeoclonium sp. and comparative coccal alga Diplosphaera chodatii on agar plates. Culture of algae grown in liquid medium were used to study subzero temperatures on the species resistance to shock freezing. Then, microalgae were frozen in liquid nitrogen and inoculated on BBM agar after thawing. Physiological status of algae was evaluated by chlorophyll fluorescence parameters during 28 days. The results showed that interspecific differences existed in their tolerance to shock freezing, as well as their consequent growth rate on agars. Direct effects of freezing in liquid nitrogen was demonstrated in chlorophyll fluorescence parameters recorded immediately after thawing the samples (in liquid medium). In spite of the fact that majority of cells was destroyed by shock freezing, the potential of photochemical processes in PS II (FV/FM) remained constant in D. chodatii. It may indicate high resistance of the species to freezing/thawing cycles and a capability of the surviving cells, core chlorophylls in PS II respectively, to perform photosynthetic processes related to PS II. Contrastingly, Stigeoclonium sp. showed a shock freezing-dependent decrease in FV/FM. When shock-frozen, thawed and inoculated on agar plates, the culture of D. chodatii, and Stigeoclonium sp. showed cultivation time-dependent increase in chlorophyll fluorescence parameters (FV/FM, FS).
ER  -

OREKHOVA, Alla, Miloš BARTÁK, Aleyna OZKAR and Josef ELSTER. The effect of shock freezing on physiological properties and consequent growth of Antarctic filamentous (Stigeoclonium sp.) and coccal alga (Diplosphaera chodatii) on agar plates. \textit{Czech Polar Reports}. Masarykova univerzita, 2019, vol.~9, No~1, p.~37-48. ISSN~1805-0689. Available from: https://dx.doi.org/10.5817/CPR2019-1-4.

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