STARÝ, Tomáš, Pavla ŠATKOVÁ, Jana PITERKOVA, Barbora MIESLEROVA, Lenka LUHOVÁ, Jaromír MIKULIK, Tomáš KAŠPAROVSKÝ, Marek PETŘIVALSKÝ and Jan LOCHMAN. The elicitin -cryptogein's activity in tomato is mediated by jasmonic acid and ethylene signalling pathways independently of elicitin-sterol interactions. Planta. New York: Springer, 2019, vol. 249, No 3, p. 739-749. ISSN 0032-0935. doi:10.1007/s00425-018-3036-1.
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Basic information
Original name The elicitin -cryptogein's activity in tomato is mediated by jasmonic acid and ethylene signalling pathways independently of elicitin-sterol interactions
Authors STARÝ, Tomáš (203 Czech Republic, belonging to the institution), Pavla ŠATKOVÁ (203 Czech Republic), Jana PITERKOVA (203 Czech Republic), Barbora MIESLEROVA (203 Czech Republic), Lenka LUHOVÁ (203 Czech Republic), Jaromír MIKULIK (203 Czech Republic), Tomáš KAŠPAROVSKÝ (203 Czech Republic, belonging to the institution), Marek PETŘIVALSKÝ (203 Czech Republic) and Jan LOCHMAN (203 Czech Republic, belonging to the institution).
Edition Planta, New York, Springer, 2019, 0032-0935.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10608 Biochemistry and molecular biology
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW Full Text
Impact factor Impact factor: 3.390
RIV identification code RIV/00216224:14310/19:00107459
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1007/s00425-018-3036-1
UT WoS 000459488700009
Keywords in English Defence-related genes; Elicitins; Pseudoidium neolycopersici; Resistance; Signalling; Sterol binding
Tags rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Marie Šípková, DiS., učo 437722. Changed: 18/3/2020 16:34.
Abstract
Main conclusionThe level of resistance induced in different tomato genotypes after -CRY treatment correlated with the upregulation of defence genes, but not sterol binding and involved ethylene and jasmonic acid signalling.Elicitins, a family of small proteins secreted by Phytophthora and Pythium spp., are the most well-known microbe-associated molecular patterns of oomycetes, a lineage of fungus-like organisms that include many economically significant crop pathogens. The responses of tomato plants to elicitin INF1 produced by Phytophthora infestans have been studied extensively. Here, we present studies on the responses of three tomato genotypes to -cryptogein (-CRY), a potent elicitin secreted by Phytophthora cryptogea that induces hypersensitive response (HR) cell death in tobacco plants and confers greater resistance to oomycete infection than acidic elicitins like INF1. We also studied -CRY mutants impaired in sterol binding (Val84Phe) and interaction with the binding site on tobacco plasma membrane (Leu41Phe), because sterol binding was suggested to be important in INF1-induced resistance. Treatment with -CRY or the Val84Phe mutant induced resistance to powdery mildew caused by the pathogen Pseudoidium neolycopersici, but not the HR cell death observed in tobacco and potato plants. The level of resistance induced in different tomato genotypes correlated with the upregulation of defence genes including defensins, -1,3-glucanases, heveins, chitinases, osmotins, and PR1 proteins. Treatment with the Leu41Phe mutant did not induce this upregulation, suggesting similar elicitin recognition in tomato and tobacco. However, here -CRY activated ethylene and jasmonic acid signalling, but not salicylic acid signalling, demonstrating that elicitins activate different downstream signalling processes in different plant species. This could potentially be exploited to enhance the resistance of Phytophthora-susceptible crops.
Links
GAP501/12/0590, research and development projectName: Charakterizace procesů zapojených v indukci resistence rostlin na patogeny s využitím elicitinů se změněnou schopností vyvolávat obranné reakce
Investor: Czech Science Foundation, Standard Projects
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